Functional analysis of proteins belongs to SIBLING family in physiological and pathological calcification
| Project/Area Number |
18590371
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Osaka University |
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Principal Investigator |
NOMURA Shintaro Osaka University, Graduate School of Frontier Bioscience, Associate Professor (80159087)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,950,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | ostemontin / mechanical stress / estrogen / transgenic mice / cell attachment / knockout mice / SIBLING / luciferase / 石灰化 / 骨、軟骨組織 / 腎臓結石 / RGD / シュウ酸カルシウム |
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| Research Abstract |
1. Identification of promoter region of Osteopontin in response to environmental change.
Up-regulation of osteopontin was observed in transgenic mice carrying GFP cDNA ligated with distal promoter (-5505 bp to mRNA start site) (GFP5505 OPN) when mechanical stress was loaded to bone. Similar effect was observed in the ovaryextomized transgenic mice. Further investigation revealed that mechanical stress response element and estrogen respose element lies between -3156 and -5505 in the start site of transcription.
2. Analysis of primary cultured cells derived from transgenic mice.
Bone cells containing osteoblasts and osteocytes of the transgenic mice were cultured. Expression level of GFP was elevated when mechanical stress was loaded to cultured cells. Identification of mechanical stress-response element was performed by luciferase assay using various region of promoter region joined to luciferase cDNA. Finally, we identified the mechanical stress-response element between-3983 and -315G to the mRNA start site of osteopontin gene.
3. Effects of truncated osteopontin on development of bone tissue.
Transgenic mice carrying either calcium binding site or cell attachment site of osteopontin controlled -5505 promoter were generated. Transgenic mice were introduced to knockout mice of osteopontin gene. Generated mice showed abnormal structure in bone. The result indicate that calcium binding site and cell attachment site of osteopontin functioning in vivo.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Saito-Ohara F, Inazawa J. Kohno T. Yokota J. Ishii S. Reduced levels of ATF-2 predispose mice to mammary tumors2007
Author(s)
Maekawa, T., Shinagawa, T., Sano, Y., Sakuma, T., Nomura, S., Nagasaki, K., Miki, Y
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Journal Title
Mol Cell Biol 27
Pages: 1730-44
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Successful formation of calcium oxalate crystal deposition in mouse kidney by intraabdominal glyoxylate injection2007
Author(s)
Okada, A., Nomura, S., Higashibata, Y., Hirose, M., Gao, B., Yoshimura, M., Itoh, Y., Yasui, T., Tozawa, K., Kohri, K
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] ATF-2 controls transcription of Maspin and GADD45alpha genes independently from p53 to suppress mammary tumors2007
Author(s)
Maekawa, T., Sano, Y., Shinagawa, T., Rahman, Z., Sakuma, T., Nomura, S., Licht, JD., Ishii, S
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Journal Title
Oncogene 27
Pages: 1045-1054
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Morphological conversion of calcium oxalate crystals into stones is regulated by osteopontin in mouse kidney
Author(s)
Okada, A., Nomura, S., Saeki, Y., Higashibata, Y., Hamamoto, S., Hirose, M., Itoh, Y., Yasui, T., Tozawa, K., Kohri, K
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Journal Title
J. Bone and Mineral Res. (in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] IL-6 blockade inhibits the induction of myelin antigen specific Th17 cells and TM cells in experimental autoimmune encephalomyelitis
Author(s)
Serada, S., Fujimoto, M., Mihara, M., Koike, N., Ohsugi, Y., Nomura, S., Yoshida, H., Nishikawa, T., Terabe, F., Ohgawara, T., Takahashi, T., Ripley, B., Kimura, A., Naka, T
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Journal Title
Proc. Natl. Acad. Sci. USA. (in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Supressor of cytokine signaling ameliorates dextran sulfate sodium-induced colitis in mice
Author(s)
Horino, J., Fujimoto, M., Terabe, F., Serada, S., Takahashi, T., Soma, Y., Tanaka, K., Chinen, T., Yoshiimura, A., Nomura, S., Kawase, I., Hayashi, N., Kishimoto, T., Naka, T
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Journal Title
Int. J. Immunol. (in press)
Description
「研究成果報告書概要(欧文)」より
Related Report
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