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Functional analysis of proteins belongs to SIBLING family in physiological and pathological calcification

Research Project

Project/Area Number 18590371
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionOsaka University

Principal Investigator

NOMURA Shintaro  Osaka University, Graduate School of Frontier Bioscience, Associate Professor (80159087)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,950,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsostemontin / mechanical stress / estrogen / transgenic mice / cell attachment / knockout mice / SIBLING / luciferase / 石灰化 / 骨、軟骨組織 / 腎臓結石 / RGD / シュウ酸カルシウム
Research Abstract

1. Identification of promoter region of Osteopontin in response to environmental change.
Up-regulation of osteopontin was observed in transgenic mice carrying GFP cDNA ligated with distal promoter (-5505 bp to mRNA start site) (GFP5505 OPN) when mechanical stress was loaded to bone. Similar effect was observed in the ovaryextomized transgenic mice. Further investigation revealed that mechanical stress response element and estrogen respose element lies between -3156 and -5505 in the start site of transcription.
2. Analysis of primary cultured cells derived from transgenic mice.
Bone cells containing osteoblasts and osteocytes of the transgenic mice were cultured. Expression level of GFP was elevated when mechanical stress was loaded to cultured cells. Identification of mechanical stress-response element was performed by luciferase assay using various region of promoter region joined to luciferase cDNA. Finally, we identified the mechanical stress-response element between-3983 and -315G to the mRNA start site of osteopontin gene.
3. Effects of truncated osteopontin on development of bone tissue.
Transgenic mice carrying either calcium binding site or cell attachment site of osteopontin controlled -5505 promoter were generated. Transgenic mice were introduced to knockout mice of osteopontin gene. Generated mice showed abnormal structure in bone. The result indicate that calcium binding site and cell attachment site of osteopontin functioning in vivo.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (17 results)

All 2008 2007 2006 Other

All Journal Article (17 results) (of which Peer Reviewed: 7 results)

  • [Journal Article] Successful formation of calcium oxalate crystal deposition in mouse kidney by intraabdominal glyoxylate injection.2008

    • Author(s)
      Okada, et. al.
    • Journal Title

      J. Bone and Mineral Res. (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] IL-6 blockade inhibits the induction of myelin antigen specific Th17 cells and Th1 cells in experimental autoimmune encephalomyelitis.2008

    • Author(s)
      Serada, et. al.
    • Journal Title

      Proc. Natl. Acad. Sci. USA. (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Supressor of cytokine signaling ameliorates dextran sulfate sodium-induced colitis in mice.2008

    • Author(s)
      Horino, et. al.
    • Journal Title

      Int. J. Immunol. (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] ATF-2 controls transcription of Maspin and GADD45alpha genes independently from p53 to suppress mammary tumors.2008

    • Author(s)
      Maekawa, et. al.
    • Journal Title

      Oncogene (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reduced levels of ATF-2 predispose mice to mammary tumors.2007

    • Author(s)
      Maekawa, et. al.
    • Journal Title

      Mol Cell Biol. 27

      Pages: 1730-1734

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Successful formation of calcium oxalate crystal deposition in mouse kidney by intraabdominal glyoxylate injection.2007

    • Author(s)
      Okada, et. al.
    • Journal Title

      Urol Res. 35

      Pages: 89-99

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] ATF-2 controls transcription of Maspin and GADD45alpha genes independently from p53 to suppress mammary tumors.2007

    • Author(s)
      Maekawa, et. al.
    • Journal Title

      Oncogene 27

      Pages: 1045-1054

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Saito-Ohara F, Inazawa J. Kohno T. Yokota J. Ishii S. Reduced levels of ATF-2 predispose mice to mammary tumors2007

    • Author(s)
      Maekawa, T., Shinagawa, T., Sano, Y., Sakuma, T., Nomura, S., Nagasaki, K., Miki, Y
    • Journal Title

      Mol Cell Biol 27

      Pages: 1730-44

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Successful formation of calcium oxalate crystal deposition in mouse kidney by intraabdominal glyoxylate injection2007

    • Author(s)
      Okada, A., Nomura, S., Higashibata, Y., Hirose, M., Gao, B., Yoshimura, M., Itoh, Y., Yasui, T., Tozawa, K., Kohri, K
    • Journal Title

      Urol Res 35

      Pages: 89-99

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] ATF-2 controls transcription of Maspin and GADD45alpha genes independently from p53 to suppress mammary tumors2007

    • Author(s)
      Maekawa, T., Sano, Y., Shinagawa, T., Rahman, Z., Sakuma, T., Nomura, S., Licht, JD., Ishii, S
    • Journal Title

      Oncogene 27

      Pages: 1045-1054

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] SOCS-2 interferes with myotube formation and potentiates osteoblast differentiation through upregulation of JunB in C2C12 cells.2006

    • Author(s)
      Ouyang X, Nomura S. et al.
    • Journal Title

      J Cell Physiol. 207(2)

      Pages: 428-436

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Osteoblast differentiation is impaired in SOCS-1-deficient mice.2006

    • Author(s)
      Abe T, Nomura S. et al.
    • Journal Title

      J Bone Miner Metab. 24(4)

      Pages: 283-290

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Morphological conversion of calcium oxalate crystals into stones is regulated by osteopontin in mouse kidney

    • Author(s)
      Okada, A., Nomura, S., Saeki, Y., Higashibata, Y., Hamamoto, S., Hirose, M., Itoh, Y., Yasui, T., Tozawa, K., Kohri, K
    • Journal Title

      J. Bone and Mineral Res. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] IL-6 blockade inhibits the induction of myelin antigen specific Th17 cells and TM cells in experimental autoimmune encephalomyelitis

    • Author(s)
      Serada, S., Fujimoto, M., Mihara, M., Koike, N., Ohsugi, Y., Nomura, S., Yoshida, H., Nishikawa, T., Terabe, F., Ohgawara, T., Takahashi, T., Ripley, B., Kimura, A., Naka, T
    • Journal Title

      Proc. Natl. Acad. Sci. USA. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Supressor of cytokine signaling ameliorates dextran sulfate sodium-induced colitis in mice

    • Author(s)
      Horino, J., Fujimoto, M., Terabe, F., Serada, S., Takahashi, T., Soma, Y., Tanaka, K., Chinen, T., Yoshiimura, A., Nomura, S., Kawase, I., Hayashi, N., Kishimoto, T., Naka, T
    • Journal Title

      Int. J. Immunol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Successful formation of calcium oxalate stone in mouse kidney by intraabdominal glyoxylate injection.

    • Author(s)
      Okada A, Nomura S. et al.
    • Journal Title

      Urol. Res. (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Reduced levels of ATF-2mice to Mammary tumors

    • Author(s)
      Maekawa T, Nomura S. et al.
    • Journal Title

      Mol. Cell Biol. (印刷中)

    • Related Report
      2006 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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