Fumctional analysis of AEPP in skeletal muscle

• Project/Area Number: 18590375
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Oita University
• Principal Investigator: MORIYAMA Masatsugu Oita University, Department of medicine, Professor (90239707)
• Co-Investigator(Kenkyū-buntansha): MATSUURA Keiko Oita University, Department of medicine, Associate Professor (00291542) ; HIJIYA Naoki Oita University, Department, ofmedicine, Assistant Professor (80305036) ; UCHIDA Tomohisa Oita University, Department of medicine, Assistant Professor (70381035)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: ARPP / CARP / muscle injury / Skeletal muscle / MARP / Nuclear translocation / Sarcomere / I band / ノックアウトマウス / 脂肪酸 / FABP / 糖代謝

Research Abstract

Ankyrin-repeat protein with a PEST motif and a proline-rich region(Arpp), also designated as Ankrd2, is a member of the muscle ankyrin repeat proteins (MARPs), which have been proposed to be involved in muscle stress response pathways. Arpp/Ankrd2 is localized mainly in the I-band of striated muscle. However, it has recently been reported that Arpp/Ankrd2 can interact with nuclear proteins, such as premyelocytic leukemia protein (PML), p53 and YB- 1 in vitro. In this study, to determine whether nuclear accumulation of Arpp/Ankrd2 actually occurs, we performed an immunohistochemical investigation of gastrocnemius muscles that had been injured by injection of cardiotoxin or contact with dry ice. We found that Arpp/Ankrd2 accumulated in the nuclei of myofibers located adjacent to severely damaged myofibers after muscle injury. Double-labeled immunohistochemistry revealed that Arpp/Ankrd2 accumulated in the nuclei of sarcomere-damaged myofibers. Furthermore, we found that Arpp/Ankrd2 tended to be localized in euchromatin where genes are transcriptionally activated. Based on these findings, we suggest that Arpp/Ankrd2 may translocate from the I-band to the nucleus in response to muscle damage and may participate in the regulation of gene expression.

Research Products

• [Journal Article] Arpp/Ankrd2,a member of the Muscle Ankyrin Repeat Proteins(MARPs),translocates from the I-band to the nucleus after muscle injury (2008)
  • Author(s): Tsukamoto Y, Hijiya N, Yano S, Yokoyama S, Nakada C, Uchida T, Matsuura K, Moriyama M.
  • Journal Title: Histochemistry and Cell Biology 129 Pages: 55-64
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Titled Arpp/Ankrd2, a member of the Muscle Ankyrin Repeat Proteins(MARPs), translocates from the Pband to the nucleus after muscle injury (2008)
  • Author(s): Y., Tsukamoto, N., Hijiya, S., Yano, S., Yokoyama, C., Nakada, T., Uchida, K., Matsuura, M., Morivama
  • Journal Title: Histochemistry and Cell Biology vol.129 Pages: 55-64
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Arpp/Ankrd2, a member of the Muscle Ankyrin Repeat Proteins (MARPs), translocates from the I-band to the nucleus after muscle injury. (2008)
  • Author(s): Tsukamoto Y, Hijiya N, Yano S, Yokoyama S, Nakada C, Uchida T, Matsuura K, Moriyama M.
  • Journal Title: Histochemistry and Cell Biology 129 Pages: 55-64
  • Peer Reviewed
• [Journal Article] Upregulated expressipn of cardiac ankyrin-repeated protein in renal podocytes is associated with proteinuria severity in lupus nephritis (2007)
  • Author(s): Matsuura K, Uesugi N, Hijiya N, Uchida T, Moriyama M
  • Journal Title: Human Pathology 38 Pages: 410-419
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] High-resolution analysis of DNA copy number alterations and the gene expression in renal clear cell carcinoma (2007)
  • Author(s): Yoshimoto T, Matsuura K, Karnan S, Tagawa H, Nakada C, Tanigawa M, Tsukamoto Y, Uchida T, Kashima K, Akizuki S, Takeuchi I, Sato F, Mimata H, Seto M, Moriyama M.
  • Journal Title: Journal of Pathology 213 Pages: 392-401
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Title : Upregulated egression of cardiac ankyriirrepeated protein in renal podocytes is associated with proteinuria severity in lupus nephritis (2007)
  • Author(s): K., Matsuura, N., Uesugi, N., Hijiya, T., Uchida, M., Morivama
  • Journal Title: Human Pathology vol.38 Pages: 410-419
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Title : High-resolution analysis of DNA copy number alterations and the gene expression in renal clear cell carcinoma (2007)
  • Author(s): T., Yosbimoto, K., Matsuura, S., Karnan, H., Tagawa, C., Nakada, M., Tanigawa, Y., Tsukamoto, T., Uchida, K., Kashima, S., Akizuki, I., Takeuchi, F., Sato, H., Mimata, M., Seto, M., Morivama
  • Journal Title: Journal of Pathology vol.213 Pages: 392-401
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Upregulated expression of cardiac ankyrin-repeated protein in renal podocytes is associated with proteinuria severity in lupus nephritis. (2007)
  • Author(s): Matsuura K, Uesugi N, Hijiya N, Uchida T and Moriyama M
  • Journal Title: Human Pathology 38 Pages: 410-419
  • Peer Reviewed
• [Journal Article] High-resolution analysis of DNA copy number alterations and the gene expression in renal clear cell carcinoma. (2007)
  • Author(s): Yoshimoto T, Matsuura K, Karnan S, Tagawa H, Nakada C, Tanigawa M, Tsukamoto Y, Uchida T, Kashima K, Akizuki S, Takeuchi I, Sato F, Mimata H, Seto M, Moriyama M.
  • Journal Title: Journal of Pathology 213 Pages: 392-401
  • Peer Reviewed
• [Journal Article] ARPP protein is selectively expressed in renal oncocytoma, but not in real cell carcinomas. (2007)
  • Author(s): Shimori K, Nagashima Y, Kuroda M, Takeda A, Tsukamoto Y, Kawamura T, Hiramatsu T, Matsuura K, Hijiya N, Yano S, Yokoyama S, Ito H, Moriyama M
  • Journal Title: Modern Pathology 20 Pages: 199-207
• [Journal Article] Acase of right isomerism showing long survival without surgery. (2007)
  • Author(s): Matsuura K, Akizuki S, Nakamura N, Ishibashi-Ueda H, Moriyama M
  • Journal Title: Southern Medical Journal 100 Pages: 218-221
• [Journal Article] Upreulated expression fo cardiac ankyrin-repeated protein in renal podocytes is associated with proteinuria severity in Iupus nephritis. (2007)
  • Author(s): Matsuura k, Uesugi N, Hijiya N, Uchida T, Moriyama M
  • Journal Title: Human Pathology 38 Pages: 410-419
• [Journal Article] Immunohistochemical diagnosis of the cagA-gene genotype of Helicodacter pylori with antj-East Asian CagA-specific antibody. (2007)
  • Author(s): Uchida T, Kanada R, Tsukamoto Y, Hijiya N, Matsuura K, Yano S, Yokoyama S, Kishida T, Kodama M, Murakami K, Fujioka T, Moriyama M
  • Journal Title: Cancer Science 98 Pages: 521-528
• [Journal Article] Extracellular signal-regulated protein kinase is activated in cervical intraepithelial neoplasms but inactivated in invasive cervical carcinoma. (2006)
  • Author(s): Matsuura K, Nohno Y, Hijiya N, Uchida T, Tsukamoto Y, Moriyama M
  • Journal Title: Pathology International 56 Pages: 368-374
  • NAID: 10020332771
• [Presentation] Title : High-resolution analysis of DNA copy number alterations and the gene expression in renal clear cell carcinoma (2008)
  • Author(s): Keiko, Matsuura, Taichiro, Yoshimoto, Sivasundaram, Karnan, Hiroyuki, Tagawa, Chisato, Nakada, Masato, Tanigawa, Yoshiyuki, Tsukamoto, Tomohisa, Uchida, IchiroTakeuchi, Fuminori, Sato, Hiromitsu, Mimata, Masao, Seto, Masatsugu, Moriyama
  • Organizer: The 67th Annual Meeting of the Japanese Cancer As sociation
  • Place of Presentation: Nagoya Congress Center
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] High-resolution analysis of DNA copy number alterations and the gene expression in renal clear cell carcinoma (2007)
  • Author(s): Keiko Matsuura, Taichiro Yoshimoto, Sivasundaram Kaman, Hiroyuki Tagawa, Chisato Nakada, Masato Tanigawa, Yoshiyuki Tsukamoto, Tomohisa Uchida, Ichiro Takeuchi, Fuminori Sato, Hiromitsu Mimata, Masao Seto, Masatsugu Moriyama
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜市(パシフィコ横浜)
  • Year and Date: 2007-10-04
  • Description: 「研究成果報告書概要(和文)」より