Development of a in vivo screening method for evaluation prostate carcinogenic potential in chemicals

• Project/Area Number: 18590377
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Nagoya City University
• Principal Investigator: SHIRAI Tomoyuki Nagoya City University, Graduate School of Medical Science, Professor (60080066)
• Co-Investigator(Kenkyū-buntansha): ASAMOTO Makoto Nagoya City University, Graduate School of Medical Science, Associate Professor (50212494) ; 鈴木 周五 名古屋市立大学, 大学院医学研究科, 研究員 (60363933)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥4,020,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: Prostate / Carcinogen / Screening method / PhIP / DMAB / Cadmium / マイクロアレイ / oncomoduline

Research Abstract

We obtained gene expressions profiles of rat ventral prostate tissue treated with three kinds of carcinogens, -amino-l-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP), 3, 2'-dimethyl-4-aminobiphenyl (DMAB)and cadmium. There were only three genes that was commonly up regulated in the 3 chemicals. Among the genes, we focused on oncomodulin and performed immunhistochemicl analysis for expression of oncomodulin in ventral prostates treated with the chemicals. Oncomodulin demonstrated sporadic positivity in cytoplasms of the prostate glands. However, there were no differences for expressions of oncomodulin between non-treated control group tissues and the 3 carcinogenic chemical groups, except for DMAB. There results indicated that the oncompdulin expression analysis may not be suitable for a in vivo screening method for prostate carcinogens.

Research Products

• [Journal Article] Optimization of an animal test protocol for toxioogenomics studies(1);Requirement study of a protocol (2007)
  • Author(s): Sumida, K.
  • Journal Title: Toxicol.Sci 32 Pages: 19-32
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Optimization of an animal test protocol for toxioogenomics studies(2);A-cross-Laboratory gene expression analysis (2007)
  • Author(s): Sumida, K.
  • Journal Title: Toxicol.Sci 32 Pages: 33-45
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Protective effects of citrus nobiletin and auraptene in transgenicrats developing adenocarcinoma of the prostate(TRAP)and human prostate carcinomacells (2007)
  • Author(s): Tang, M.
  • Journal Title: Cancer Sci 98 Pages: 471-477
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Optimization of an animal test protocol for toxicogenomics studies (1); Requirement study of a protocol (2007)
  • Author(s): Sumida, K., Saito, K., Oeda, K., Otsuka, M., Tsujimura, K., Miyaura, H., Asamoto, M., Shirai, T
  • Journal Title: Toxicol. Sci. 32 Pages: 19-32
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Optimization ofan animal test protocol for toxicogenomics studies (2); A-cross-Laboratory gene expression analysis (2007)
  • Author(s): Sumida, K., Saito, K., Oeda, K., Otsuka, M., Tsujimura, K., Miyaura, H., Asamoto, M., Shirai, T
  • Journal Title: Toxicol. Sci. 32 Pages: 33-45
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Protective effects of citrus nobiletin and auraptene in transgenic rats developing adenocarcinoma of the prostate (TRAP) and human prostate carcinoma cells (2007)
  • Author(s): Tang, M., Ogawa, K., Asamoto, M., Hokaiwado, N., Azman, S., Suzuki, S., Takahashi, S., Tanaka, T., Ichikawa, K., Shirai, T
  • Journal Title: Cancer Sci. 98 Pages: 471-477
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Optimization of an animal test protocol for toxicogenomics studies(1);Requirement study of a protocol. (2007)
  • Author(s): Sumida, K.
  • Journal Title: Toxicol.Sci. 32 Pages: 19-32
• [Journal Article] Optimization of an animal test protocol for toxicogenomics studies(2);A-cross-Laboratory gene expression analysis. (2007)
  • Author(s): Sumida, K.
  • Journal Title: Toxicol.Sci. 32 Pages: 33-45
• [Journal Article] Protective effects of citrus nobiletin and auraptene in transgenic rats developing adenocarcinoma of the prostate(TRAP) and human prostate carcinoma cells. (2007)
  • Author(s): Tang, M.
  • Journal Title: Cancer Sci. 98 Pages: 471-477
• [Journal Article] Pitfalls with MRI Evaluation of Prostate Cancer Detection (2006)
  • Author(s): Okamura, T.
  • Journal Title: Urol.Int. 77 Pages: 301-306
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Differences in gen expression profiles in the liver between carcinogenic and non-carcinogenic isomers of compounds given to ratina 28-day repeat-dose toxicity study (2006)
  • Author(s): Nakayama, K.
  • Journal Title: Toxicol.Appl.Pharmacol 217 Pages: 299-307
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Prediction of carcinogenic potential by a toxicogenomic approachusingrat hepatoma cells (2006)
  • Author(s): Tsujimura, K.
  • Journal Title: Cancer Sci 97 Pages: 1002-1010
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Pitfalls with MRI Evaluation of Prostate Cancer Detection (2006)
  • Author(s): Okamura, T., Uemoto, Y., Yamashita, K., Suzuki, S., Shirai, T., Hashimoto, Y., Kohri, K
  • Journal Title: Urol. Int 77 Pages: 301-306
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Differences in gene expression profiles in the liver between carcinogenic and non-carcinogenic isomers of compounds given to rat in a 28-day repeat-dose toxicity study (2006)
  • Author(s): Nakayama, K., Kawano, Y., Kawakami, Y., Moriwaki, N., Masaru, S., Otsuka, M., Yakabe, Y., Miyaura, H., Saito, K., Sumida, K., Shirai, T
  • Journal Title: Toxicol. Appl. Pharmacol 217 Pages: 299-307
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Prediction of carcinogenic potential by a toxicogenomic approach using rat hepatoma cells (2006)
  • Author(s): Tsujimura, K., Asamoto, M., Shugo, S., Hokaiwado, N., Ogawa, K., Shirai, T
  • Journal Title: Cancer Sci. 97 Pages: 1002-1010
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Pitfalls with MRI Evaluation of Prostate Cancer Detection. (2006)
  • Author(s): Okumura, T.
  • Journal Title: Urol.Int. 77 Pages: 301-306
• [Journal Article] Differences in gene expression profiles in the liver between carcinogenic and non-carcinogenic isomers of compounds given to rat in a 28-day repeat-dose toxicity study. (2006)
  • Author(s): Nakayama, K.
  • Journal Title: Toxicol. Appi. Pharmacol. 217 Pages: 299-307
• [Journal Article] Prediction of carcinogenic potential by a toxicogenomic approach using rat hepatoma cells. (2006)
  • Author(s): Tsujimura, K.
  • Journal Title: Cancer Sci. 97 Pages: 1002-1010
• [Presentation] cDNAアレイを用いた前立腺発がん物質に特異的な遺伝子の検索 (2008)
  • Author(s): 二口 充
  • Organizer: 第24回日本毒性病理学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-02-07
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] cDNA microarray analysis of genes which specifically alteredin the rat prostate after treatment with prostate carcinogens (2008)
  • Author(s): Futakuchi, M., Sato, S., Sumida, K., Saito, K., Takahashi, S., Asamoto, M., Shirai, T
  • Organizer: The 24th Annual Meeting of the Japanese Society of Toxicologic Pathology
  • Place of Presentation: Nagoya
  • Year and Date: 2008-02-07
  • Description: 「研究成果報告書概要(欧文)」より