| Project/Area Number |
18590387
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Aichi Medical University |
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Principal Investigator |
IWASHITA Toshihide Aichi Medical University, Medicine, Associate professor (00283432)
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| Co-Investigator(Kenkyū-buntansha) |
KUROKAWA Kei Aichi medical University, Medicine, Research Associate (90399030)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Neural crest / Stem cells / ヒルシュスプルング病 |
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| Research Abstract |
We have postulated that Hox proteins were candidate for the intrinsic factors that make difference between vagal neural crest and trunk neural crest. To understand the molecular mechanism of the neural crest differentiation by Hox protein, Hox genes were over-expressed in the migrating neural crest. Especially, we focused on the function of HoxB8 that was predominantly expressed by vagal neural crest. HoxB8 and GFP were over-expressed in the migrating chicken vagal neural crest derived from posterior hindbrain using ovo-electroporation technique. The neural crest expressing HoxB8 and GFP protein was also stained by anti-HNK1 antibody that specifically recognized neural crest. Interestingly, Ret, a receptor of glial cell-line derived neurotrophic factor and played an important role on migration of vagal neural crest to gut, was much suppressed in the neural crest expressing HoxB8.
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