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New recognition of Leishmania major by infected macrophages

Research Project

Project/Area Number 18590401
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Parasitology (including Sanitary zoology)
Research InstitutionNagasaki University

Principal Investigator

HONMA Kiri  Nagasaki University, Graduate school of Biomedical Sciences, Lecturer (70307940)

Co-Investigator(Kenkyū-buntansha) YUI Ktsuyuki  Nagasaki University, Graduate school of Biomedical Sciences, Professor (90274638)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,020,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsIRF-4 / proinflammatory cvtokines / Leishmania major / macronhages / L.major
Research Abstract

(A)When L.major infected to IRF-4 deficient macrophages in vivo, footpad thickness increased at the early stage (2-5 weeks after infection)compared to wild type.
(B)The rate of the L.major infection was same between IRF-4 deficient macrophages and wild-type cells. However, the proportion of the infected macrophages remarkably decreased compared to wild type when L.major infected to IRF-4 deficient macrophages for 48 hrs in vitro, suggested that IRF-4 negatively regulated exclusion of L.major from the infected macrophages. TNF-α and NO from IRF-4 deficient, L.major-infected macrophages produced much higher than wild-type. Interestingly, IL-12 from IRF-4 deficient, and L.major infected macrophages produced much lower than wild-type. We expected that signaling to recognize L.major was different from TLR signaling because we have already reported that both IL-12 and TNF-α production through TLR remarkably enhanced in IRF-4 deficient macrophages. Alternation of cytokine production in IRF-4 KO macrophages to infect with L.major regulated at the transcriptional level. Various kind of denatured L.major antigens were made to identify major L.major molecule. Heat denatured or Proteinase K treated L.major antigen did not affect cytokine production pattern of TNF-α or IL-12 in IRF-4 KO macrophages. L.major molecule contained in insoluble fraction and was Proteinase K resistant molecule. It suggested that major antigen was not protein. And it was impossible to use ion exchange chromatography such as Bio-Cad.
(C)To separate L.major antigen, gel filtration method have been performed because it was able to separate each molecules by molecular weight.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (14 results)

All 2007 2006 2005

All Presentation (14 results)

  • [Presentation] Interferon regulatory factor-4 negatively regulates IL-4 production of naive CD4 T cells2007

    • Author(s)
      本間 季里
    • Organizer
      日本免疫学会
    • Place of Presentation
      グランドプリンスホテル新高輪
    • Year and Date
      2007-11-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] NB排虫に重要なTh2サイトカイン産生におけるIRF-4の役割2007

    • Author(s)
      本間 季里
    • Organizer
      日本寄生虫学会
    • Place of Presentation
      大阪大学
    • Year and Date
      2007-03-29
    • Related Report
      2007 Annual Research Report
  • [Presentation] N.brasiliensis排虫に重要なTh2サイトカイン産生におけるIRF-4の役割2007

    • Author(s)
      本間 季里
    • Organizer
      第76回日本寄生虫学会大会
    • Place of Presentation
      大阪
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Interferon regulatory facor-4(IRF-4)negatively regulater IL-4 production of naive CD4 T cells2007

    • Author(s)
      Kiri Honma
    • Organizer
      第37回日本免疫学会学術集会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The role of IRF-4 in Th2 cytokine production to eliminate N. brasiliensis.2007

    • Author(s)
      Kiri, Honma
    • Organizer
      The 76th Annual Meeting of the Japanese Society of Parasitology
    • Place of Presentation
      Osaka
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Interferon regulatory factor-4(IRF-4) negatively modulates IL-4 production from naive CD4 T cells.2007

    • Author(s)
      Kiri, Honma
    • Organizer
      The 37th Annual Meeting of the Japanese Society for Immunology
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Leishmania major感染マクロファージの原虫排除おけるInterferon regulatori factor-4(IRF-4)の役割2006

    • Author(s)
      本間 季里
    • Organizer
      第75回日本寄生虫学会大会
    • Place of Presentation
      弘前
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Leishmania majorおよびNippostrongylus brasiliensis感染防御におけるIRF-4の役割2006

    • Author(s)
      木村 大輔
    • Organizer
      第75回日本寄生虫学会大会
    • Place of Presentation
      弘前
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Interferon regulatory factor-4(IRF-4)negatively modulates IL-4 production from naive CD4 T cells2006

    • Author(s)
      Kiri Honma
    • Organizer
      第36回日本免疫学会術集会
    • Place of Presentation
      大阪
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] IRF-4 negatively regulates cytokine production by macrophages in response to TLR2006

    • Author(s)
      Katsuyuki Yui
    • Organizer
      Keystone symposia
    • Place of Presentation
      Keystone,Colorado
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Interferon regulatory factor-4(IRF-4) negatively modulates IL-4 production from naive CD4 T cells.2006

    • Author(s)
      Kiri, Honma
    • Organizer
      The 36th Annual Meeting of the Japanese Society for Immunology
    • Place of Presentation
      Osaka
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] IRF-4 negatively regulates cytokine production by macrophages in response to TLR.2006

    • Author(s)
      Katsuyuki, Yui
    • Organizer
      The 36th Keystone symposia
    • Place of Presentation
      Keystone, Colorado, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The role of IRF-4 to eliminate L.major from the infected Macrophages.2005

    • Author(s)
      Kiri, Honma
    • Organizer
      The 75th Annual Meeting of the Japanese Society of Parasitology
    • Place of Presentation
      Hirosaki
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The role of IRF-4 to protect Leishmania major and Nippostrongyrus brasiliensis infection.2005

    • Author(s)
      Daisuke, Kimura
    • Organizer
      The 75th Annual Meeting of the Japanese Society of Parasitology
    • Place of Presentation
      Hirosaki
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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