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Analysis of the effects of the disruption of the mitochondrial antioxidant protein gene on development of malaria parasite

Research Project

Project/Area Number 18590412
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Parasitology (including Sanitary zoology)
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

KAWAZU Shin-Ichiro  Obihiro University of Agriculture and Veterinary Medicine, National Research Center for Protozoan Diseases, Professor (60312295)

Co-Investigator(Kenkyū-buntansha) KANO Shigeyuki  International Medical Center of Japan, Research Institute, Department Director (60233912)
TORII Motomi  Ehime University, Medical School, Professor (20164072)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsInfectious diseases / Malaria / Mitochondria / Redox / Transgenic parasite
Research Abstract

In this project, we investigated the physiologic role of mitochondrial 2-Cys peroxiredoxin (TPx-2) in malaria parasite by disrupting the gene in the human malaria parasite Plasmodium falciparum. The gene-disrupted parasite (Pf TPx-2 KO) developed normally in human erythrocytes and multiplied at a rate similar to that of the parent strain (FCR-3). The in vitro growth of Pf TPx-2 KO was inhibited by tBOOH and alternative oxidases inhibitors (Salicylhydroxamic acid and n-propyl gallate) at concentrations similar to those inhibit the growth of FCR-3 strain. To investigate the importance of TPx-2 in the parasite life cycle in vivo, we tried to disrupt the homologous-gene in the rodent malaria parasite Plasmodium berghei. We constructed the gene disruption plasmid using pMD204, which was provided by Malaria Research and Reference Reagent Resource (MR4). We also prepared anti-recombinant PbTPx-2 rabbit serum for analyzing phenotype of the disruptant. The KO experiment is currently under way in our laboratory. Further studies clarifying the roles of TPx-2 will provide new insight into the biology of malaria parasites and facilitate development of strategies to interrupt their life cycle.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (31 results)

All 2008 2007 2006 Other

All Journal Article (11 results) (of which Peer Reviewed: 6 results) Presentation (18 results) Remarks (2 results)

  • [Journal Article] Peroxiredoxins in malaria parasites: Parasitologic aspects.2008

    • Author(s)
      Kawazu, S. , et. al.
    • Journal Title

      Parasitol. Int. 57

      Pages: 1-7

    • NAID

      10020150795

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Evaluation of blood preservation methods in the performance of the who in vitro micro-test for Plasmodium falciparum in the field.2008

    • Author(s)
      Iwagami, M. , et. al.
    • Journal Title

      Trop. Med. Health. (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Disruption of the Plasmodium berghei 2-Cys peroxiredoxin TPx-1 gene hinders the sporozoite developmetn in the vector mosquito.2008

    • Author(s)
      Yano, K. , et. al.
    • Journal Title

      Mol. Biochem. Parasitol. (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Peroxiredoxins in malaria parasites: Parasitologic aspects.2008

    • Author(s)
      Kawazu, S., et. al.
    • Journal Title

      Parasitology International 57(1)

      Pages: 1-7

    • NAID

      10020150795

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Evaluation of blood preservation methods in the. performance of the who in vitro micro-test for Plasmodium falciparum in the field2008

    • Author(s)
      Iwagami, M., et. al.
    • Journal Title

      Tropical Medicine and Health (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Disruption of the Plasmodium berghei 2-Cys peroxiredoxin TPx-1 gene hinders the sporozoite development in the vector mosquito.2008

    • Author(s)
      Yano, K., et. al.
    • Journal Title

      Molecular & Biochemical Parasitology (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Peroxiredoxins in malaria parasites: Parasitologic aspects.2008

    • Author(s)
      Kawazu, S., et. al.
    • Journal Title

      Parasitol. Int. 57

      Pages: 1-7

    • NAID

      10020150795

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of blood preservation methods in the performance of the who in vitro micro-test for Plasmodium falciparum in the field.2008

    • Author(s)
      Iwagami, M., et. al.
    • Journal Title

      Trop. Med. Health. (掲載決定)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Disruption of the Plasmodium berghei 2-Cys peroxiredoxin TPx-1 gene hinders the sporozoite development in the vector mosquito.2008

    • Author(s)
      Yano, K., et. al.
    • Journal Title

      Mol. Biochem. Parasitol. (掲載決定)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analyses of cytochrome b mutation in Plasmodium falciparum isolates in Thai-Myanmar border.2007

    • Author(s)
      Naoshima-Ishibashi, Y., et al.
    • Journal Title

      Travel Med. Infect. Dis. 5

      Pages: 132-134

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Mitochondria and apicoplast of Plasmodium falciparum : Behaviour on subcellular fractionation and the implication.2007

    • Author(s)
      Kobayashi, T., et al.
    • Journal Title

      Mitochondrion 7

      Pages: 125-132

    • Related Report
      2006 Annual Research Report
  • [Presentation] Disruption of 2-Cys peroxiredoxin TPx-1 gene in Plasmodium berghei hinders the sporozoite development.2007

    • Author(s)
      Kawazu, S. , et. al.
    • Organizer
      American Society of Tropical Medicine and Hygiene 56th Annual Meeting
    • Place of Presentation
      Philadelphia Marriott Downtown, Pennsylvania, USA
    • Year and Date
      2007-11-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Disruption of 2-Cys peroxiredoxin TPx-1 gene in Plasmodium berghei hinders the sporozoite development.2007

    • Author(s)
      Kawazu, S., et. al.
    • Organizer
      American Society of Tropical Medicine and Hygiene 56th Annual Meeting
    • Place of Presentation
      Philadelphia, Pennsylvania, USA
    • Year and Date
      2007-11-05
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 2-Cys型ペルオキシレドキシン(TPx-1)ノックアウトがマラリア原虫の哺乳類体内での発育に及ぼす影響の解析2007

    • Author(s)
      河津信一郎, 他
    • Organizer
      第6回分子寄生虫・マラリア研究フォーラム
    • Place of Presentation
      松山市男女共同参画推進センター「コムズ」
    • Year and Date
      2007-10-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Analyse of effects of gene TPx-1 disruption on the development of liver stage malaria parasite in the mammalian host.2007

    • Author(s)
      Kawazu, S., et. al.
    • Organizer
      The 6th Forum on Molecular Parasitology and Malaria Researches
    • Place of Presentation
      Matsuyama, Japan
    • Year and Date
      2007-10-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 抗酸化タンパク2-Cys型ペルオキシレドキシン(Prx)ノックアウトがマラリア原虫のオーシスト発育に及ぼす影響の解析2007

    • Author(s)
      河津信一郎, 他
    • Organizer
      第143回日本獣医学会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2007-04-04
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Analyse of effects of Anti-oxidant gene TPx-1 disruption on the development of oocyst stage malaria parasites in the mosquito.2007

    • Author(s)
      Kawazu, S., et. al.
    • Organizer
      The 143th Annual Meeting of the Japanese Society of Veterinary Science
    • Place of Presentation
      Tsukuba, Japan
    • Year and Date
      2007-04-04
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 細胞質およびミトコンドリアに局在するペルオキシレドキシンの遺伝子をノックアウトした2種類の熱帯熱マラリア原虫株の作製2007

    • Author(s)
      河津信一郎, 他
    • Organizer
      第76回日本寄生虫学会
    • Place of Presentation
      大阪大学コンベンションセンター
    • Year and Date
      2007-03-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Targeted disruption of cytosolic 1-Cys peroxiredoxin gene and mitochondrial thioredoxin peroxidase-2 gene in Plasmodium falciparum.2007

    • Author(s)
      Kawazu, S., et. al.
    • Organizer
      The 76th Annual Meeting of the Japanese Society of Parasitology
    • Place of Presentation
      Osaka, Japan
    • Year and Date
      2007-03-29
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 2-Cys peroxiredoxin is involved in gametocyte development in plasmodium berghei.2006

    • Author(s)
      Kawazu, S. , et. al.
    • Organizer
      The 12th Korea-Japan Parasitologist's Seminar (Forum Cheju-12)
    • Place of Presentation
      Tokyo University, Tokyo, Japan
    • Year and Date
      2006-11-06
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 2-Cys peroxiredoxin is involved in gametocyte development in Plasmodium berghei.2006

    • Author(s)
      Kawazu, S., et. al.
    • Organizer
      The 12th Korea-Japan Parasitologist's Seminar (Forum Cheju-12)
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2006-11-06
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] マラリア原虫細胞質でチオレドキシンと相互作用するタンパク質の同定2006

    • Author(s)
      河津信一郎, 他
    • Organizer
      第5回分子寄生虫・マラリア研究フォーラム
    • Place of Presentation
      東京大学医学部
    • Year and Date
      2006-10-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Identification of proteins targeted by cytoplasmic thioredoxin in Plasmodium falciparum.2006

    • Author(s)
      Kawazu, S., et. al.
    • Organizer
      The 5th Forum on Molecular Parasitology and Malaria Researches
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2006-10-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 2-Cys peroxiredoxin TPx-l is involved in gametocyte development in Plasmodium berghei.2006

    • Author(s)
      Kawazu, S. , et. al.
    • Organizer
      The 15 th Japanese-German Symposium on Protozoan Diseases
    • Place of Presentation
      Obihiro University, Hokkaido, Japan
    • Year and Date
      2006-09-18
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 2-Cys peroxiredoxin TPx-1 is involved in gametocyte development in Plasmodium berghei.2006

    • Author(s)
      Kawazu, S., et. al.
    • Organizer
      The 15th Japanese-German Symposium on Protozoan Diseases
    • Place of Presentation
      Obihiro, Japan
    • Year and Date
      2006-09-18
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Peroxiredoxin is involved in gametocyte development in Plasmodium berghei.2006

    • Author(s)
      Kawazu, S. , et. al.
    • Organizer
      The 11th International Congress of Parasitology (ICOPA XI)
    • Place of Presentation
      SECC Glasgow, Scotland, UK
    • Year and Date
      2006-08-11
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Peroxiredoxin is involved in gametocyte development in Plasmodium berghei.2006

    • Author(s)
      Kawazu, S., et. al.
    • Organizer
      The 11th International Congress of Parasitology (ICOPA XI)
    • Place of Presentation
      Glasgow, Scotland, UK
    • Year and Date
      2006-08-11
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 2-Cys型ペルオキシレドキシン(TPx-1)ノックアウトがマラリア原虫のオーシスト発育に及ぼす影響の解析2006

    • Author(s)
      矢野和彦, 他
    • Organizer
      第75回日本寄生虫学会
    • Place of Presentation
      弘前文化センター
    • Year and Date
      2006-05-19
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analyse of effects of TPx-1 disruption on the development of oocyst stage malaria parasites in the mosquito.2006

    • Author(s)
      Yano, K., et. al.
    • Organizer
      The 75th Annual Meeting of the Japanese Society of Parasitology
    • Place of Presentation
      Hirosaki, Japan
    • Year and Date
      2006-05-19
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.obihiro.ac.jp/~tryp/index.html

    • Related Report
      2007 Final Research Report Summary
  • [Remarks]

    • URL

      http://www.obihiro.ac.jp/~tryp/index.html

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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