Analysis of the effects of the disruption of the mitochondrial antioxidant protein gene on development of malaria parasite
| Project/Area Number |
18590412
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Obihiro University of Agriculture and Veterinary Medicine |
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Principal Investigator |
KAWAZU Shin-Ichiro Obihiro University of Agriculture and Veterinary Medicine, National Research Center for Protozoan Diseases, Professor (60312295)
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| Co-Investigator(Kenkyū-buntansha) |
KANO Shigeyuki International Medical Center of Japan, Research Institute, Department Director (60233912)
TORII Motomi Ehime University, Medical School, Professor (20164072)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Infectious diseases / Malaria / Mitochondria / Redox / Transgenic parasite |
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| Research Abstract |
In this project, we investigated the physiologic role of mitochondrial 2-Cys peroxiredoxin (TPx-2) in malaria parasite by disrupting the gene in the human malaria parasite Plasmodium falciparum. The gene-disrupted parasite (Pf TPx-2 KO) developed normally in human erythrocytes and multiplied at a rate similar to that of the parent strain (FCR-3). The in vitro growth of Pf TPx-2 KO was inhibited by tBOOH and alternative oxidases inhibitors (Salicylhydroxamic acid and n-propyl gallate) at concentrations similar to those inhibit the growth of FCR-3 strain. To investigate the importance of TPx-2 in the parasite life cycle in vivo, we tried to disrupt the homologous-gene in the rodent malaria parasite Plasmodium berghei. We constructed the gene disruption plasmid using pMD204, which was provided by Malaria Research and Reference Reagent Resource (MR4). We also prepared anti-recombinant PbTPx-2 rabbit serum for analyzing phenotype of the disruptant. The KO experiment is currently under way in our laboratory. Further studies clarifying the roles of TPx-2 will provide new insight into the biology of malaria parasites and facilitate development of strategies to interrupt their life cycle.
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Report
(3 results)
Research Products
(31 results)
