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THE SIGNALING SYSTEM COORDINATING OF BACTERIAL BEHAVIOR IN HUMAN PATHOGENIC VIBRIOS

Research Project

Project/Area Number 18590424
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionOkayama University

Principal Investigator

MIYOSHI Shinhchi  Okayama University, GRADUATE SCHOOL OF MEDICINE, DENTISTRY AND PHARMACEUTCAL SCIENCES, PROFESSOR (60182060)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,960,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsPathogenic vibrios / Gene expression / Quorum-sennsing / Hemolysin / Metalloprotease / プロテアーゼ / 毒素遺伝子 / 筋密度依存性調節
Research Abstract

1. The presence of the quorum-sensing regulation system in Vibrio mimicus, a causative agent of food poisoning, was investigated. The culture supernatants of V. mimicus strains were found to possess Al-2 autoinducer like activity, and the strains were found to harbor the genes, which are homologous to luxS, luxO, and luxR of V harveyi. These genes of V hatveyi have been shown to be important components of V harveyi-like quorum-sensing system. The luxO gene homologue known to encode LuxO, the central component of the regulation system, was disrupted, and effects on protease and hemolysin activity were studied. Disruption of IuxO gene resulted in the increased protease activity, but the hemolysin activity did not vary considerably.
2. In Vibrio vulnificus that causes septicemia in humans, the extracellular proteins regulated by the quorum-sensing system were studied. The overall analysis of the proteins from a wild type strain and from a luxO mutant was carried out, and their profiles were compared. Production of more than 10 extracellular proteins was significantly affected by the genetic disruption of the luxO gene, indicating that the LuxO protein may be a global regulator in the quorum-sensing system. However, the mRNA levels of the proteins were not varied in the mutant. Therefore, the LuxO protein may regulate the translation process of the proteins.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (52 results)

All 2008 2007 2006 2005 Other

All Journal Article (22 results) (of which Peer Reviewed: 8 results) Presentation (29 results) Book (1 results)

  • [Journal Article] Differential gene expression and extracellular secretion of the collagenolytic enzymes by the pathogen Vibrio parahaemolyticus2008

    • Author(s)
      Miyoshi, Shin-ichi
    • Journal Title

      FFMS Microbiology Letters 283(in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Variation of extracellular proteases produced by Vibrio vulnificus clinical isolates: genetic diversity of the metalloprotease gene (vvp), and serine protease secretion by vvp-negative strains2008

    • Author(s)
      Wang, Jiyou
    • Journal Title

      Microbial Pathogenesis 45(in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The crucial amino acid residue related to inactivation of Vibrio vulnifirus hemolysin2008

    • Author(s)
      Senoh, Mitsutoshi
    • Journal Title

      Microbial Pathogenesis 44

      Pages: 78-83

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The crucial amino aci residue related to inactivation of Vibrio vulnificus hemolysin2008

    • Author(s)
      Senoh, Mitsutoshi
    • Journal Title

      Microbial Pathogenesis 44

      Pages: 78-83

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The crucial amino acid residue related to inactivation of Vibrio vulnificus hemolysin2008

    • Author(s)
      Senoh, Mitsutoshi
    • Journal Title

      Microbial Pathogenesis 44

      Pages: 78-83

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Haemolysin produced by Vibrio mimicus activates two Cl secretory pathways in cultured intestinal-like Caco-2 cells2007

    • Author(s)
      Takahashi, Akira
    • Journal Title

      Cellular Microbiology 9

      Pages: 583-595

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Growth phase dependent activation of the precursor of Vibrio mimicus hemolysin (Pro-VMH)2007

    • Author(s)
      Sultan, Zafar
    • Journal Title

      Journal of Health Science 53

      Pages: 430-434

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Haemolysin produced by Vibrio mimicus activates two CI secretory pathways in cultured intestinal-like Caco-2 cells2007

    • Author(s)
      Takahashi, Akira
    • Journal Title

      Cellular Microbiology 9

      Pages: 583-595

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Presence of LuxS/AI-2 based quorum-sensing system in Vibrio mimicus: Lux0 controls protease activity2006

    • Author(s)
      Sultan, Zafar
    • Journal Title

      Microbiology and Immnology 50

      Pages: 407-417

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Growth phase-dependent production of a toxic metalloprotease by Vibrio vulnificus2006

    • Author(s)
      Miyoshi, Shin-ichi
    • Journal Title

      Toxin Reviews 25

      Pages: 19-30

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Vibrio vulnificus infection and metalloprotease2006

    • Author(s)
      Miyoshi, Shin-ichi
    • Journal Title

      Journal of Deramatology 33

      Pages: 589-595

    • NAID

      10020339361

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Proteolytic activation of Vibrio mimicus (Vm) major outer membrane protein haemaggluyinin (HA) with Vm-HA/protease: implication for understanding bacterial adherence2006

    • Author(s)
      Alam, Munirul
    • Journal Title

      Microbiology and Immunology 50

      Pages: 845-850

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Presence of LuxS/A1-2 based quorum-sensing system in Vibrio mimicus : LuxO controls protease activity2006

    • Author(s)
      Sultan, Zafar
    • Journal Title

      Microbiology and Immunology 50

      Pages: 407-417

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Vibrio vulnificus infection and metalloprotease2006

    • Author(s)
      Miyoshi, Shin-ich
    • Journal Title

      Journal of Dermatology 33

      Pages: 589-595

    • NAID

      10020339361

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Proteolytic activation of Vibfio mimicus (Vm) major outer membrane protein haemagglutinin (HA) with Vm-HA/proteae : implication for understanding bacterial adherence2006

    • Author(s)
      Alam, Munirul
    • Journal Title

      Microbiology and Immunology 50

      Pages: 845-850

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Chapter44 Hemolysins of Vibrio cho/erae and other Vibrio apecies. p748-762, in Alouf JE, Popoff MR (eds), The comprehensive sourcebook of bacterial protein toxins2006

    • Author(s)
      Shinoda, Sumio
    • Journal Title

      Elsevier Academic Press, (London)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Presence of LuxS/AI-2 based quorum-sensing system in Vibrio mimicus : LuxO controls protease activity2006

    • Author(s)
      Sultan, Zafar
    • Journal Title

      Microbiology and Immunology 50(5)

      Pages: 407-417

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Vibrio vulnificus infection and metalloprotease2006

    • Author(s)
      Miyoshi, Shin-ichi
    • Journal Title

      Journal of deramatology 33(9)

      Pages: 589-595

    • NAID

      10020339361

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Growth phase-dependent production of a toxic metalloprotease by Vibrio vulnificus2005

    • Author(s)
      Miyoshi, Shin-ichi
    • Journal Title

      Toxin Reviews 25(1)

      Pages: 19-30

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Differential gene expression and extracellular secretion of the collagenolytic enzymes by the pathogen Vibfio parahaemolyticus

    • Author(s)
      Miyoshi, Shin-ichi
    • Journal Title

      FEMS Microbiology Letters (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Variation of extracellular proteases produced by Vibrio vulnificus clincal isolates : genetic diversity of the metalloprotease gene (vvp), and serine protease production by vvp-negative strains

    • Author(s)
      Wang, Jiyou
    • Journal Title

      Microbial Pathogenesis (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Growth phase dependent activation of the precursor of Vibrio mimicus hemolysin (pro-VMH)

    • Author(s)
      Sultan, Zafar
    • Journal Title

      Joumal of Health Science 53

      Pages: 430-434

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Vibrio vulnificus 臨床分離株のプロテアーゼの多様性2007

    • Author(s)
      王, 継有
    • Organizer
      第46回日本薬学会 中国四国支部学術大会
    • Place of Presentation
      高知市
    • Year and Date
      2007-11-10
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Variation of the protease in Vibrio vulnificus clinical strains2007

    • Author(s)
      Wang, Jiyou
    • Organizer
      The 46th Annual Meeting of the Pharmaceutical Society of Japan, Chugoku-Shikoku Branch
    • Place of Presentation
      Kochi
    • Year and Date
      2007-11-10
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Vibrio vulnificus臨床分離株のプロテアーゼの多様性2007

    • Author(s)
      王 継有
    • Organizer
      第46回日本薬学会 中国四国支部学術大会
    • Place of Presentation
      高知市
    • Year and Date
      2007-11-10
    • Related Report
      2007 Annual Research Report
  • [Presentation] Vibrio mimicus溶血毒素の成熟化に関与するプロテアーゼ2007

    • Author(s)
      水野, 環
    • Organizer
      フォーラム2007 衛生薬学・環境トキシコロジー
    • Place of Presentation
      大阪市
    • Year and Date
      2007-11-01
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] A protease mediating maturation of Vibrio mimicus hemolysin2007

    • Author(s)
      Mizuno, Tamaki
    • Organizer
      Forum 2007 Toxicology in Health and Environmental Chemistry
    • Place of Presentation
      Osaka
    • Year and Date
      2007-11-01
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Vibrio mimicusヘモリジンの金属プロテアーゼによる溶血活性の変化2007

    • Author(s)
      水野, 環
    • Organizer
      第54回毒素シンポジウム
    • Place of Presentation
      泉佐野市
    • Year and Date
      2007-09-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Effect of a metalloprotease on the activity of Vibrio mimicus hemolysin2007

    • Author(s)
      Mizuno, Tamaki
    • Organizer
      The 54th Symposium on Toxins
    • Place of Presentation
      IzumiSano
    • Year and Date
      2007-09-05
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Pore formation of Vibrio mimicus hemolysin in lipid bilayers2007

    • Author(s)
      Takahashi, Akira
    • Organizer
      107th Annual Meeting of American Society for Microbiology
    • Place of Presentation
      Toronto, Canada
    • Year and Date
      2007-05-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Pore formation of Vibrio mimicus hemolysin in lipid bilayers2007

    • Author(s)
      Takahashi, Akira
    • Organizer
      The 107th Annual Meeting of American Society for Microbiology
    • Place of Presentation
      Toronto, Canada
    • Year and Date
      2007-05-21
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Control of protease activity by Lux0 in Vibrio/mimicus: evidence of the presence of active V. harveyi-like quorum-sensing network2006

    • Author(s)
      Sultan, Zafar
    • Organizer
      41st Joint Conference on Cholera and Other Bacterial Enteric infections Panel
    • Place of Presentation
      京都市
    • Year and Date
      2006-12-08
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Control of protease activity by LuxO in Vibrio mimicus : evidence of the presence of active V. harveyi-like quorum-sensing network2006

    • Author(s)
      Sultan, Zafar
    • Organizer
      The 41st Joint Conference on Cholera and Other Bacterial Enteric infections Panel
    • Place of Presentation
      Kyouto
    • Year and Date
      2006-12-08
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Vibrio mimicusヘモリジンの限定分解と活性の変化2006

    • Author(s)
      水野, 環
    • Organizer
      第40回腸炎ビブリオシンポジウム
    • Place of Presentation
      新宿区
    • Year and Date
      2006-11-30
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effect of the limited proteolysis on the activity of Vibrio mimicus hemolysin2006

    • Author(s)
      Mizuno, Tamaki
    • Organizer
      The 40th Symposium on Vibrio parahaemolyticus
    • Place of Presentation
      Tokyo
    • Year and Date
      2006-11-30
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The crucial amino acid residues for stability and activity of Vibrio vulnificus hemolysin2006

    • Author(s)
      Miyoshi, Shin-ichi
    • Organizer
      International Symposium of Vibrio vulnificus and its infectious diseases
    • Place of Presentation
      Tainan, Taiwan
    • Year and Date
      2006-10-13
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The crucial amino acid residues for stability and activity of Vibrio vulnificus hemolysin2006

    • Author(s)
      Miyoshi, Shin-ichi
    • Organizer
      The International Symposium of Vibrio vulnificus and its infectious diseases
    • Place of Presentation
      Tainan, Taiwan
    • Year and Date
      2006-10-13
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] vibrio vulnificusのヘム利用系の特異性2006

    • Author(s)
      佐々木, とも子
    • Organizer
      第18回微生物シンポジウム
    • Place of Presentation
      岡山市
    • Year and Date
      2006-09-01
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] vibrio choleraeの選択的単離法の開発2006

    • Author(s)
      近江, 友香
    • Organizer
      第18回微生物シンポジウム
    • Place of Presentation
      岡山市
    • Year and Date
      2006-09-01
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The specificity of the heme uptake system in Vibrio vulnificus2006

    • Author(s)
      Sasaki, Tomoko
    • Organizer
      The 18th Symposium on Microbiology
    • Place of Presentation
      Okayama
    • Year and Date
      2006-09-01
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of the specific method for isolation of Vibrio cholerae2006

    • Author(s)
      Omi, Yuka
    • Organizer
      The 18th Symposium on Microbiology
    • Place of Presentation
      Okayama
    • Year and Date
      2006-09-01
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Proteolytic inactivation of an enterotoxic hemolysin produced by Vibrio mimicus2006

    • Author(s)
      Mizuno, Tamaki
    • Organizer
      Food Micro 2006
    • Place of Presentation
      Bologna, Italy
    • Year and Date
      2006-08-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Amino acid residues relevant for hemolytic activity of Vibrio vulnifius hemolysin2006

    • Author(s)
      Senoh, Mitsutoshi
    • Organizer
      Food Micro 2006
    • Place of Presentation
      Bologna, Italy
    • Year and Date
      2006-08-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Virulence factor(s) produced by Vibrio mimicus2006

    • Author(s)
      Kobayashi, Akiko
    • Organizer
      Food Micro 2006
    • Place of Presentation
      Bologna, Italy
    • Year and Date
      2006-08-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Amino acid residues relevant for hemolytic activity of Vibrio vulnit7us hemolysin2006

    • Author(s)
      Senoh, Mitsutoshi
    • Organizer
      Food Micro 2006
    • Place of Presentation
      Bologna, Italy
    • Year and Date
      2006-08-29
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Vibrio vulnificus hemolysinの活性発現に重要なアミノ酸残基2006

    • Author(s)
      妹尾, 充敏
    • Organizer
      第53回毒素シンポジウム
    • Place of Presentation
      北杜市
    • Year and Date
      2006-07-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Vibrio mimicusの病原因子の探索2006

    • Author(s)
      小林, 晃子
    • Organizer
      第53回毒素シンポジウム
    • Place of Presentation
      北杜市
    • Year and Date
      2006-07-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The amino acid residues important for the activity of Vibrio vulnificus hemolysin2006

    • Author(s)
      Senoh, Mitsutoshi
    • Organizer
      The 53rd Symposium on Toxins
    • Place of Presentation
      Hokuto
    • Year and Date
      2006-07-05
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Studies on novel virulence factors produced by Vibrio mimicus2006

    • Author(s)
      Kobayashi, Akiko
    • Organizer
      The 53rd Symposium on Toxins
    • Place of Presentation
      Hokuto
    • Year and Date
      2006-07-05
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 新規抗菌薬の開発を指向したヘム利用系の解析2006

    • Author(s)
      佐々木, とも子
    • Organizer
      日本防菌防黴学会第33回年次大会
    • Place of Presentation
      品川市
    • Year and Date
      2006-05-01
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Studies on the bacterial heme uptake system for a target of a novel antibacterial agent2006

    • Author(s)
      Sasaki, Tomoko
    • Organizer
      The 33rd annual meeting of the Society for antibacterial and antifungal agents
    • Place of Presentation
      Tokyo
    • Year and Date
      2006-05-01
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] The comprehensive sourcebook of bacterial protein toxins2006

    • Author(s)
      Shinoda, Sumio
    • Publisher
      Elsevier Academic Press
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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