Roles of Monocytes/Macrophage for Acquired Immunity in Helicobacter pylori Infection

• Project/Area Number: 18590425
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Okayama University
• Principal Investigator: YOKOTA Kenji Okayama University, Graduate School of Health Science, Associate Professor (00243460)
• Co-Investigator(Kenkyū-buntansha): AYADA Kiyoshi Okayama University, Graduate school of Medicine, Dentistry Pharmaceutical Scrence, Assistant Professor (00379835) ; OGUMA Keiji Okayama University, Graduate school of Medicine, Dentistry Pharmaceutical science, Professor (00002262)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: Helicobacter pylori / macrophage / DEC205 / MAPK / TLR / HSP60 / 動脈硬化

Research Abstract

We have been studying functions of macrophage in Hefrcobacter pylori inffection. Because we think that acquired immunity may play important roles in the diseases caused by H. pylori infection. To investigate immunity of H.pylori infection, we have shown the H.pylori- HSP60 (heat shock protein 60 kDa) activates macrophage and it is useful antigen.
We have shown some results supporting by this Grant.
1) HSP60 stimulated and activated a macrophage though TLR2 and TLR4. IL8 was produced from the macrophage depending on MAPK and NE-〓B activation.
2) Arteriosclerosis in Apo-E KO mouse was progressed by H. pylori infection. The pathology was associated with immunity to HSP60 and T helper 1.
3) Patients with arteriosclerosis possessed antibody to peptides from H. pylori-HSP60. The peptides might be available for diagnosis of arteriosclerosis.
4) DNA chip study indicated that DEC205 is an important receptor for macrophage activation in patients with gastritis caused by H pylori infection. We are now studying function of DEC205 on macrophage using NOMO-1 cell differenciated by PMA.

Research Products

• [Journal Article] Chronic infections and atherosclerosis (2007)
  • Author(s): Ayada K, Yokota K, Kobayashi K, Shoenfeld Y, Matsuura E, Oguma K.
  • Journal Title: Ann N YAcad Sci. 1108 Pages: 594-602
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Antibodies against heat shock protein 60 derived from Helicobacter pylori: diagnostic implications hl cardiovascular disease. (2007)
  • Author(s): Okada T, Ayada K, Usui S, Yokota K, Cui J, Kawahara Y, Inaba T, Hirohata S, Mizuno M, Yamamoto D, Kusachi S, Matsuura E, Oguma K.
  • Journal Title: J Autoimmun 29 Pages: 106-115
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Conversion of Flavodoxin from holoenayme to apoprotein during growth phase changes in Helicobacter pylori (2007)
  • Author(s): Shimomura H, Hayashi S, Yokota K, Oguma K, Hirai Y.
  • Journal Title: J Bacteriol 189 Pages: 4960-4963
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Helicobacter pylori heat-shock protein 60 induces interleukin-8 via a Toll-like receptor (TLR) 2 and mitogen-activated protein (MAP) kinase pathway in human monocytes (2007)
  • Author(s): Zhao Y, Yokota K, Ayada K, Yamamoto Y, Okada T, Shen L, Oguma K.
  • Journal Title: J Med. Microbiol. 56 Pages: 154-164
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Baseline gastric mucosal atrophy is a risk factor associated with the development of gastric cancer after Helicobacter pylori eradication therapy in patients with peptic ulcer diseases. (2007)
  • Author(s): Take S, Mizuno M, Ishiki K, Nagahara Y, Yoshida T, Yokota K, Oguma K.
  • Journal Title: J Gastroenterol. 42 Pages: 21-27
  • NAID: 10018539759
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Chronic infections and atherosclerosis (2007)
  • Author(s): Ayada, K., Yokota, K., Kobayashi, K., Shoenfeld, Y., Matsuura, E., Oguma, K
  • Journal Title: Ann N Y Acad Sof 1108 Pages: 594-602
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Antibodies against heat shock protein 60 derived from Helicobacter pylori diagnostic implications in cardiovascular disease (2007)
  • Author(s): Okada, T., Ayada, K., Usui, S., Yokota, K., Cui, J., Kawahara, Y., Inaba, T., Hirohata, S., Mizuno, M., Yamamoto, D., Kusachi, S., Matsuura, E., Oguma, K
  • Journal Title: J Autoimmun 29 Pages: 106-115
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Conversion of flavodoxin from holoenzyme to apoprotein during growth phase changes in Helicobacter pylori (2007)
  • Author(s): Shimomura, H., Hayashi, S., Yokota, K., Oguma, K., Hirai, Y
  • Journal Title: J Bacteriol 189 Pages: 4960-4963
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Helicobacter pylori heat-shock protein 60 induces interleokin-8 via a Toll-like receptor (TLR)2 and mitogen-activated protein (MAP) kinase pathway in human monocytes (2007)
  • Author(s): Zhao, Y., Yokota, K., Ayada, K., Yamamoto, Y., Okada, T., Shen, L., Oguma, K
  • Journal Title: J Med Microbiol 56 Pages: 154-164
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Baseline gastric mucosal atrophy is a risk factor associated with the development of gastric Cancer after Helicobacter pylori eradication therapy in patients with peptic ulcer diseases (2007)
  • Author(s): Take, S., Mizuno, M., Ishiki, K., Nagahara, Y., Yoshida, T., Yokota, K., Oguma, K
  • Journal Title: J Gastroenterol 42 Pages: 21-27
  • NAID: 10018539759
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Chronic infections and atherosclerosis (2007)
  • Author(s): Ayada K, Yokota K, Kobayashi K, Shoenfeld Y, Matsuura E, Oguma K.
  • Journal Title: Ann N Y Acad Sci. 1108 Pages: 594-602
  • Peer Reviewed
• [Journal Article] Antibodies against heat shock protein 60 derived from Helicobacter pylori: diagnostic implications in cardiovascular disease. (2007)
  • Author(s): Okada T, Ayada K, Usui S, Yokota K, Cui J, Kawahara Y, Inaba T, Hirohata S, Mizuno M, Yamamoto D, Kusachi S, Matsuura E, Oguma K.
  • Journal Title: J Autoimmun. 29 Pages: 106-115
  • Peer Reviewed
• [Journal Article] Conversion of flavodoxin from holoenzyme to apoprotein during growth phase changes in Helicobacter pylori (2007)
  • Author(s): Shimomura H, Hayashi S, Yokota K, Oguma K, Hirai Y.
  • Journal Title: J Bacteriol. 189 Pages: 4960-4963
  • Peer Reviewed
• [Journal Article] Helicobacter pylori heat-shock protein 60 induces interleukin-8 via a Toll-like receptor (TLR)2 and mitogen-activated protein (MAP) kinase pathway in human monocytes (2007)
  • Author(s): Zhao Y, Yokota K, Ayada K, Yamamoto Y, Okada T, Shen L, Oguma K.
  • Journal Title: J Med Microbiol. 56 Pages: 154-164
  • Peer Reviewed
• [Journal Article] Helicobacter pylori heat-shock protein 60 induces interleukin-8 via a Toll-like receptor (TLR)2 and mitogen-activated protein (MAP) kinase pathway in human monocytes (2007)
  • Author(s): Zhao Y, Yokota K, Ayada K, Yamamoto Y, Okada T, Shen L, Oguma K
  • Journal Title: J Med Microbiol. 56 Pages: 154-164
• [Journal Article] Baseline gastric mucosal atrophy is a risk factor associated with the development of gastric cancer after Helicobacter pylori eradication therapy in patients with peptic ulcer diseases (2007)
  • Author(s): Take S, Mizuno M, Ishiki K, Nagahara Y, Yoshida T, Yokota K, Oguma K
  • Journal Title: J Gastroebterol. 42 Pages: 21-27
  • NAID: 10018539759
• [Presentation] H. pylori-感染におけるマンノーズレセプターファミリーDEC205の役割 (2008)
  • Author(s): 藤本聖人、横田憲治、綾田潔、平井一行、林俊治、平井義一、小熊恵二
  • Organizer: 第81回 日本細菌学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-03-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Role of DEC205 in H. pylori Infection (2008)
  • Author(s): Fujimoto, K., Yokota, K., Ayada, K., Hirai, K., Hyashi, K., Hirai, K., Oguma, K
  • Organizer: 80th Nippon Saikin Gakkai
  • Place of Presentation: KYOTO
  • Year and Date: 2008-03-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] H. pylori-感染におけるマンノースレセプターファミリーDEC205の役割 (2008)
  • Author(s): 藤本聖人、横田憲治、綾田潔、平井一行、林俊治、平井義一、小熊惠二
  • Organizer: 第81回 日本細菌学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-03-25
• [Presentation] Expression of endocytoric receptor DEC205 in macrophagei Helicobacter pylori infection (2007)
  • Author(s): Yokota K, Zhao Y, Fujimoto K, Ayada K, Hirai K, Oguma K
  • Organizer: 107th American Society for Microbiology General Meeting
  • Place of Presentation: Tronto
  • Year and Date: 2007-05-24
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Expression of endocytoric receptor DEC205 in macrophagei Helicobacter pylori infection. (2007)
  • Author(s): Yokota K, Zhao Y, Fujimoto K, Ayada K, Hirai K, Oguma K
  • Organizer: 107th American Society for Microbiology General Meeting
  • Place of Presentation: Tronto
  • Year and Date: 2007-05-24
• [Presentation] Expression of DEC205 stimulated with H. pylori antigens (2006)
  • Author(s): Fujimoto, K., Yokota, K., Ayada, K., Hirai, K., Hyashi, S., Hirai, K., Oguma, K
  • Organizer: 81thNippon Saikin Gakkai
  • Place of Presentation: OSAKA
  • Year and Date: 2006-03-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Expression of endocytoric receptor DEC205 in mdcrophagei Helicobacter pylori infection
  • Author(s): Yokita, K., Zhao, Y., Fiijirnoto, K., Ayada, K., Hirai, K., Ogama, K
  • Organizer: 107th General Meeting Society for Microbiology
  • Place of Presentation: TORONT
  • Description: 「研究成果報告書概要(欧文)」より
• [Patent(Industrial Property Rights)] 動脈硬化の検査法 (2007)
  • Inventor(s): 綾田潔, 横田憲治, 小熊惠二
  • Industrial Property Rights Holder: 綾田潔, 横田憲治, 小熊惠二
  • Industrial Property Number: 2006-330367
  • Acquisition Date: 2007-04-20
• [Patent(Industrial Property Rights)] 動脈硬化の検査法 (2006)
  • Inventor(s): 綾田潔, 横田憲治, 小熊恵二
  • Industrial Property Rights Holder: 国立大学法人岡山大学
  • Industrial Property Number: 2006-330367
  • Filing Date: 2006-12-07
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 動脈硬化の検査方法 (2006)
  • Inventor(s): 綾田 潔, 横田憲治, 小熊恵二
  • Industrial Property Rights Holder: 綾田 潔, 横田憲治, 小熊恵二
  • Industrial Property Number: 2006-330367
  • Filing Date: 2006-12-07