Identification of cholera toxin B subunit binding protein and its bioactive analysis
| Project/Area Number |
18590430
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Nagasaki University |
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Principal Investigator |
WADA Akihiro Nagasaki University, Institute of Tropical Medicine, Lecturer (70253698)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
|
| Budget Amount *help |
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | cholera toxin / CT-B / receptor / immune modulation / GM1 |
|---|
| Research Abstract |
The Cholera toxin (CT)is composed of two subunits, a toxigenic A subunit (CT-A)which activates the adenylyl cyclase system and a pentamaric B subunit (CT-B)which is responsible for CT binding to the cell membrane GM1 gangliosides. The CT is one of the most effective and widely studied mucosal adjuvants. Although the ADP-ribosylating CT-A has been implicated in augmenting immune responses, the receptor-binding CT-B has a greater immunogenicity and may be a repository of adjuvant activity without potential toxicity In general knowledge, the only CT-B receptor is the cell membrane GM1 ganglioside, which expresses in all mammalian cells. The GM1 may be required for the ability of CT-B molecules to alter immunoresponse. However, novel unknown CT-B receptor, which is significant for immune modulation, may account for this signal transduction. In order to elucidate mechanisms of immune modulation by CT-B in vitro, we purified cell surface CT-B binding receptor p32 (32 kDa protein)on SDS-PAGE gel by pull-down method with biotinyl CT-B and avidin-Sepharose. By in-gel digestion of p32 and MALDI-MS fingerprint analysis, p32 was determined as a well-known protein on mammalian cells. The role of CT-B receptor p32 for immune modulation by CT-B will continue to be examined.
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] Clustering of Helicobacter pylori VacA in lipid rafts, mediated by its receptor, receptor-like protein tyrosine phosphatase beta, is required for intoxication in AZ-521 Cells2006
Author(s)
M., Nakayama, J., Hisatsune, E., Yamasaki, Y., Nishi, A., Wada, H., Kurazono, J., Sap, K., Yahir, J., Moss, T., Hirayama
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Journal Title
Infect Immun 74(12)
Pages: 6571-80
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Endoscope disinfection using chlorine dioxide in an automated washer-disinfector2006
Author(s)
H., Isomoto, M., Urata, K., Kawazoe, J., Matsuda, Y., Nishi, A., Wada, K., Ohnita, Y., Hirakata, N., Matsuo, K., Inoue, T., Hirayama, S., Kamihira, S., Kohno
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Journal Title
J Hosp Infect 63(3)
Pages: 298-305
Description
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[Journal Article] Helicobacter pylori vacuolating cytotoxin induces activation of the proapoptotic proteirs Bax and Bak, leading to cytochrome c release and cell death, independent of vacuolation2006
Author(s)
E., Yamasaki, A., Wada, A., Kumatori, I., Nakagawa, J., Funao, M., Nakayama, J., Hisatsune, M., Kimura, J., Moss, T., Hirayama
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Journal Title
J Biol Chem 281(16)
Pages: 11250-9
Description
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