Research Project
Grant-in-Aid for Scientific Research (C)
The Cholera toxin (CT)is composed of two subunits, a toxigenic A subunit (CT-A)which activates the adenylyl cyclase system and a pentamaric B subunit (CT-B)which is responsible for CT binding to the cell membrane GM1 gangliosides. The CT is one of the most effective and widely studied mucosal adjuvants. Although the ADP-ribosylating CT-A has been implicated in augmenting immune responses, the receptor-binding CT-B has a greater immunogenicity and may be a repository of adjuvant activity without potential toxicity In general knowledge, the only CT-B receptor is the cell membrane GM1 ganglioside, which expresses in all mammalian cells. The GM1 may be required for the ability of CT-B molecules to alter immunoresponse. However, novel unknown CT-B receptor, which is significant for immune modulation, may account for this signal transduction. In order to elucidate mechanisms of immune modulation by CT-B in vitro, we purified cell surface CT-B binding receptor p32 (32 kDa protein)on SDS-PAGE gel by pull-down method with biotinyl CT-B and avidin-Sepharose. By in-gel digestion of p32 and MALDI-MS fingerprint analysis, p32 was determined as a well-known protein on mammalian cells. The role of CT-B receptor p32 for immune modulation by CT-B will continue to be examined.
All 2008 2007 2006
All Journal Article (11 results) (of which Peer Reviewed: 4 results) Presentation (1 results)
Cancer Research Joumal (in press)
Cancer Research Journal (in press)
医生電顕技術誌 21(1)
Pages: 1-6
J Biol Chem. 281(16)
Pages: 11250-11259
Infect Immun 74(12)
Pages: 6571-80
J Hosp Infect 63(3)
Pages: 298-305
J Biol Chem 281(16)
Pages: 11250-9
Infect.Immun. 74(12)
Pages: 6571-6580
J.Hosp.Infect. 63(3)
J.Biol.Chem. 281(16)
