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Identification of cholera toxin B subunit binding protein and its bioactive analysis

Research Project

Project/Area Number 18590430
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionNagasaki University

Principal Investigator

WADA Akihiro  Nagasaki University, Institute of Tropical Medicine, Lecturer (70253698)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordscholera toxin / CT-B / receptor / immune modulation / GM1
Research Abstract

The Cholera toxin (CT)is composed of two subunits, a toxigenic A subunit (CT-A)which activates the adenylyl cyclase system and a pentamaric B subunit (CT-B)which is responsible for CT binding to the cell membrane GM1 gangliosides. The CT is one of the most effective and widely studied mucosal adjuvants. Although the ADP-ribosylating CT-A has been implicated in augmenting immune responses, the receptor-binding CT-B has a greater immunogenicity and may be a repository of adjuvant activity without potential toxicity In general knowledge, the only CT-B receptor is the cell membrane GM1 ganglioside, which expresses in all mammalian cells. The GM1 may be required for the ability of CT-B molecules to alter immunoresponse. However, novel unknown CT-B receptor, which is significant for immune modulation, may account for this signal transduction. In order to elucidate mechanisms of immune modulation by CT-B in vitro, we purified cell surface CT-B binding receptor p32 (32 kDa protein)on SDS-PAGE gel by pull-down method with biotinyl CT-B and avidin-Sepharose. By in-gel digestion of p32 and MALDI-MS fingerprint analysis, p32 was determined as a well-known protein on mammalian cells. The role of CT-B receptor p32 for immune modulation by CT-B will continue to be examined.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (12 results)

All 2008 2007 2006

All Journal Article (11 results) (of which Peer Reviewed: 4 results) Presentation (1 results)

  • [Journal Article] Oncogenesis and the link between inflammation and cancer due to human papillomavirus(HPV)infection,and the development of vaccine control strategies2008

    • Author(s)
      Senba M., Mori N., Wada A.
    • Journal Title

      Cancer Research Joumal (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Oncogenesis and the link between inflammation and cancer due to human papillomavirus(HPV) infection, and the development of vaccine control strategies2008

    • Author(s)
      M., Senba, N., Mori, A., Wada
    • Journal Title

      Cancer Research Journal (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Oncogenesis and the link between inflammation and cancer due to human papillomavirus (HPV) infection, and the development of vaccine control strategies2008

    • Author(s)
      Senba M., Mori N., and Wada A.
    • Journal Title

      Cancer Research Journal (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 燐タングステン酸およびモリブデン酸アンモニウムによるグラム陰性菌のネガティブ染色法の改良2007

    • Author(s)
      一ノ瀬 昭豊、和田 昭裕
    • Journal Title

      医生電顕技術誌 21(1)

      Pages: 1-6

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Helicobacter pylori vacoulating cytotoxin induces activation of the proapoptoic proteins Bax and Bak,leading to cytochrome c release and cell death,independent of vacuolation2006

    • Author(s)
      Yamasaki, E., Wada, A., et. al.
    • Journal Title

      J Biol Chem. 281(16)

      Pages: 11250-11259

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Clustering of Helicobacter pylori VacA in lipid rafts, mediated by its receptor, receptor-like protein tyrosine phosphatase beta, is required for intoxication in AZ-521 Cells2006

    • Author(s)
      M., Nakayama, J., Hisatsune, E., Yamasaki, Y., Nishi, A., Wada, H., Kurazono, J., Sap, K., Yahir, J., Moss, T., Hirayama
    • Journal Title

      Infect Immun 74(12)

      Pages: 6571-80

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Endoscope disinfection using chlorine dioxide in an automated washer-disinfector2006

    • Author(s)
      H., Isomoto, M., Urata, K., Kawazoe, J., Matsuda, Y., Nishi, A., Wada, K., Ohnita, Y., Hirakata, N., Matsuo, K., Inoue, T., Hirayama, S., Kamihira, S., Kohno
    • Journal Title

      J Hosp Infect 63(3)

      Pages: 298-305

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Helicobacter pylori vacuolating cytotoxin induces activation of the proapoptotic proteirs Bax and Bak, leading to cytochrome c release and cell death, independent of vacuolation2006

    • Author(s)
      E., Yamasaki, A., Wada, A., Kumatori, I., Nakagawa, J., Funao, M., Nakayama, J., Hisatsune, M., Kimura, J., Moss, T., Hirayama
    • Journal Title

      J Biol Chem 281(16)

      Pages: 11250-9

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Clustering of Helicobacter pylori VacA in lipid rafts, mediated by its receptor, receptor-like protein tyrosine phosphatase beta, is required for intoxication in AZ-521 cells.2006

    • Author(s)
      Nakayama M et al.
    • Journal Title

      Infect.Immun. 74(12)

      Pages: 6571-6580

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Endoscope disinfection using chlorine dioxide in an automated washer-disinfector2006

    • Author(s)
      Isomoto H et al.
    • Journal Title

      J.Hosp.Infect. 63(3)

      Pages: 298-305

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Helicobacter pylori vacuolating cytotoxin induces activation of the proapoptotic proteins Bax and Bak, leading to cytochrome c release and cell death, independent of vacuolation.2006

    • Author(s)
      Yamasaki E et al.
    • Journal Title

      J.Biol.Chem. 281(16)

      Pages: 11250-11259

    • Related Report
      2006 Annual Research Report
  • [Presentation] コレラ毒素Bサブユニットが示す生物活性の解析2008

    • Author(s)
      和田 昭裕, ら
    • Organizer
      第81回日本細菌学会
    • Place of Presentation
      京都
    • Year and Date
      2008-03-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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