• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of induction and activation mechanism of protective CD8 T cells against pulmonary tuberculosis and application for the development of anti-tuberculosis vaccine

Research Project

Project/Area Number 18590431
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionUniversity of the Ryukyus

Principal Investigator

MATSUZAKI Goro  University of the Ryukyus, Center of Molecular Biosciences, Professor (30229455)

Co-Investigator(Kenkyū-buntansha) ARAKAWA Takeshi  University of the Ryukyus, Center of Molecular Biosciences, Associate Professot (50305190)
UMEMURA Masayuki  University of the Ryukyus, Center of Molecular Biosciences, Assistant Professor (90359985)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,140,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥540,000)
Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsMycobacterium / immunity / CD8+T cell / antigenic peptide / host defence to bacteria / CD8T細胞
Research Abstract

Although importance of CD8+T cells in protection against pulmonary tuberculosis has been reported using CD8-depleted or CD8+T cell-deficient mice, it is not clear how migration and activation of the mycobacterial Ag-specific T cells are regulated in the Mycobacterium tuberculosis (Mtb)-infected lung. To address the issue, we generated pentamer of MHC H-2D^b and mycobacterial Ag Mtb32a epitope complex (D^<b->Mtb32a 5mer). Using the reagent we identified mycobacterial Mtb32a-specific CD8+T cells from day 21 in the lung and from day 28 in the mediastinal lymph node after Mtb lung infection. In contrast, Mycpbacterium bovis BCG, a vaccine strain, also express Mtb32a epitope identical to that of Mtb, and BCG subcutaneous infection induced D^b-Mtb32a 5mer-stained CD8+T cells from day 7 which is earlier than that observer after Mtb lung infection. When BCG vaccinated mice were infected with Mtb in the lung 30 days after the vaccination, induction of Mtb32a-specific CD8+T cells in the lung accelerated and clearly detected from day 7 after the infection. Furthermore, the increase of the Mtb32a-specificT cells parallel to increase of interferon-gamma production in the lung in response to Mtb32a peptide. The results suggest that induction of Mtb-specific CD8+T cells delays in the lung of Mtb-infected lung, and BCG vaccination accelerate the induction with interferon-gamma production.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (11 results)

All 2008 2007 2006 Other

All Journal Article (9 results) (of which Peer Reviewed: 4 results) Presentation (2 results)

  • [Journal Article] Mucosal immunization with recombinant heparin-binding hae magglutinin adhesin suppresses extrapulmonary dissemination of Mycobacterium bovis bacillus Calmette-Guerin (BCG) in infected mice.2008

    • Author(s)
      Kohama, K., et al.
    • Journal Title

      Vaccine 26

      Pages: 924-932

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Mucosal immunization with recombinant heparin-binding haemagglutinin adhesin suppresses extrapulmonary dissemination of Mycobacterium bovis bacillus Calmette-Guerin (BCG) in infected mice.2008

    • Author(s)
      Kohama, K., Umemura, M., Okamoto, Y, Yahagi, A., Goga, H., Harakuni, T., Matsuzaki, G, Arakawa, T.
    • Journal Title

      Vaccine 26

      Pages: 924-932

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Importance of murine Vδ1^+ γδ T cells expressing interferon-γ and interleukin-17A in innate protection against Listeria monocytogenes infection.2008

    • Author(s)
      Hamada, S., et. al.
    • Journal Title

      Immunology (印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mucosal immunization with recombinant heparin-binding haemagglutinin adhesin suppresses extrapulmonary dissemination of Mycobacterium bovis bacillus Calmette-Guerin (BCG) in infected mice.2008

    • Author(s)
      Kohama, K., et. al.
    • Journal Title

      Vaccine 26

      Pages: 924-932

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Suppression of the bacterial antigen-specific T cell response and the dendritic cell migration to the lymph nodes by osteopontin.2007

    • Author(s)
      Begum, M. D., Umemura, M., Kon, S., Yahagi, A., Hamada, S., Oshiro, K., Gotoh, K., Nishizono, A., Uede, T., Matsuzaki, G.
    • Journal Title

      Microbiol. Immunol. 51(1)

      Pages: 135-147

    • NAID

      10020282178

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] IL-17-mediated regulation of innate and acquired immune response against pulmonary Mycobacterium bovis bacille Calmette-Guerin infection.2007

    • Author(s)
      Umemura, M., Yahagi, A., Hamada, S., Begum, M. D., Watanabe, H., Kawakami, K., Suda, T. Sudo, K., Nakae, S., Iwakura, Y., Matsuzaki, G.
    • Journal Title

      J. Immunol. 178 (6)

      Pages: 3786-3796

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] IL-17-mediated regulation of innate and acquired immune response against pulmonary Mycobacterium bovis bacilli Calmette-Guerin2007

    • Author(s)
      Masayuki Umemura
    • Journal Title

      Journal of Immunology 178・6

      Pages: 3786-3796

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Importance of murine Vδ1^+γδ T cells expressing interferon-γ and interleukin-17A in innate protection against Listeria monocytogenes infection.

    • Author(s)
      Hamada, S., et al.
    • Journal Title

      Immunology (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Importance of murine Vδ1^+ γδ T cells expressing interferon-γ and interleukin-17A in innate protection against Listeria monocytogenes infection.

    • Author(s)
      Hamada, S., Umemuram M., Shiono, T., Hara, H., Kishihara, K., Tanaka, K., Mayuzumi, H., Ohta, T., Goro Matsuzaki.
    • Journal Title

      Immunology (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] IL-17-mediated regulation of innate and acquired immune response against pulmonary mycobacterial infection.2007

    • Author(s)
      Umemura M, et al.
    • Organizer
      13th International Congress of Mucosal Immunology.
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] IL-17-mediated regulation of innate and acquired immune response against pulmonary mycobacterial infection.2006

    • Author(s)
      Umemura M, Yahagi, A., Hamada, S., Matsuzaki, G.
    • Organizer
      The American Association of Immunology Annual Meeting Immunology 2006
    • Place of Presentation
      Boston, MA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi