Analysis of induction and activation mechanism of protective CD8 T cells against pulmonary tuberculosis and application for the development of anti-tuberculosis vaccine
| Project/Area Number |
18590431
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
MATSUZAKI Goro University of the Ryukyus, Center of Molecular Biosciences, Professor (30229455)
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| Co-Investigator(Kenkyū-buntansha) |
ARAKAWA Takeshi University of the Ryukyus, Center of Molecular Biosciences, Associate Professot (50305190)
UMEMURA Masayuki University of the Ryukyus, Center of Molecular Biosciences, Assistant Professor (90359985)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,140,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥540,000)
Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Mycobacterium / immunity / CD8+T cell / antigenic peptide / host defence to bacteria / CD8T細胞 |
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| Research Abstract |
Although importance of CD8+T cells in protection against pulmonary tuberculosis has been reported using CD8-depleted or CD8+T cell-deficient mice, it is not clear how migration and activation of the mycobacterial Ag-specific T cells are regulated in the Mycobacterium tuberculosis (Mtb)-infected lung. To address the issue, we generated pentamer of MHC H-2D^b and mycobacterial Ag Mtb32a epitope complex (D^<b->Mtb32a 5mer). Using the reagent we identified mycobacterial Mtb32a-specific CD8+T cells from day 21 in the lung and from day 28 in the mediastinal lymph node after Mtb lung infection. In contrast, Mycpbacterium bovis BCG, a vaccine strain, also express Mtb32a epitope identical to that of Mtb, and BCG subcutaneous infection induced D^b-Mtb32a 5mer-stained CD8+T cells from day 7 which is earlier than that observer after Mtb lung infection. When BCG vaccinated mice were infected with Mtb in the lung 30 days after the vaccination, induction of Mtb32a-specific CD8+T cells in the lung accelerated and clearly detected from day 7 after the infection. Furthermore, the increase of the Mtb32a-specificT cells parallel to increase of interferon-gamma production in the lung in response to Mtb32a peptide. The results suggest that induction of Mtb-specific CD8+T cells delays in the lung of Mtb-infected lung, and BCG vaccination accelerate the induction with interferon-gamma production.
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Report
(3 results)
Research Products
(11 results)
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[Journal Article] Mucosal immunization with recombinant heparin-binding haemagglutinin adhesin suppresses extrapulmonary dissemination of Mycobacterium bovis bacillus Calmette-Guerin (BCG) in infected mice.2008
Author(s)
Kohama, K., Umemura, M., Okamoto, Y, Yahagi, A., Goga, H., Harakuni, T., Matsuzaki, G, Arakawa, T.
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Journal Title
Vaccine 26
Pages: 924-932
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Suppression of the bacterial antigen-specific T cell response and the dendritic cell migration to the lymph nodes by osteopontin.2007
Author(s)
Begum, M. D., Umemura, M., Kon, S., Yahagi, A., Hamada, S., Oshiro, K., Gotoh, K., Nishizono, A., Uede, T., Matsuzaki, G.
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Journal Title
Microbiol. Immunol. 51(1)
Pages: 135-147
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] IL-17-mediated regulation of innate and acquired immune response against pulmonary Mycobacterium bovis bacille Calmette-Guerin infection.2007
Author(s)
Umemura, M., Yahagi, A., Hamada, S., Begum, M. D., Watanabe, H., Kawakami, K., Suda, T. Sudo, K., Nakae, S., Iwakura, Y., Matsuzaki, G.
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Journal Title
J. Immunol. 178 (6)
Pages: 3786-3796
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Importance of murine Vδ1^+ γδ T cells expressing interferon-γ and interleukin-17A in innate protection against Listeria monocytogenes infection.
Author(s)
Hamada, S., Umemuram M., Shiono, T., Hara, H., Kishihara, K., Tanaka, K., Mayuzumi, H., Ohta, T., Goro Matsuzaki.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
Related Report
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