• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Functional analysis of Salmonella type III secretion system translocator protein SipC and application of vaccine development

Research Project

Project/Area Number 18590435
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionKitasato University

Principal Investigator

DANBARA Hirofumi  Kitasato University, School of Pharmacy, Department of Microbiology, Professor (40114558)

Co-Investigator(Kenkyū-buntansha) OKADA Nobuhiko  Kitasato University, School of Pharmacy, Department of Microbiology, Associate professor (80194364)
HANEDA Takeshi  Kitasato University, School of Pharmacy, Department of Microbiology, Assistant Professor (00348591)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,110,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsSalmonella / virulence factors / infectious diseases / vaccine / Tyne III secretion system
Research Abstract

Type III secretion system (T3SS) is a common virulence determinant in Gram-negative bacteria and the genetic information is often clustered in pathogenicity islands or on virulence plasmids. We have analyzed the T3SS encoded by Salmonella pathogenicity island 1 (SPI-1) that is required for epithelial cell invasion of Salmonella enterica serovar Typhimurium (S. Typhimurium). SipC is a 409-amino-acid protein that is secreted by a SPI-1-encoded T3SS in S. Typhimurium. SipC is required for S. Typhimurium to translocate across the host plasma membrane a set of secreted effector proteins that function to counteract immune signaling responses and to induce host actin rearrangement. SipC contains two predicted transmembrane helices (residues 122 to 140 and 173 to 192) that are thought to insert into the host plasma membrane during translocation. To elucidate the importance of membrane insertion for SipC function, SipC proteins containing deletions and point-mutations in the transmembrane. domain were constructed. S. Typhimurium strains expressing the mutant SipC proteins were used to infect epithelial cells. Effector translocation, pore formation, and host-cell-signaling responses were studied. Introduction of deletions and point-mutations (S165P and S165A) into the transmembrane resion of SipC resulted in a nonfunctional protein that was not secreted by the type III machinery. In addition, these SipC mutant derivatives were defective for association with host cell membranes. Furthermore, a mutant Salmonella strain that expresses the SipC derivative defective in host cell membrane insertion failed to cause severe colitis in a mouse infection model. These results strongly suggest that all functions of SipC involve insertion into host membrane.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (29 results)

All 2008 2007 2006

All Journal Article (6 results) (of which Peer Reviewed: 3 results) Presentation (23 results)

  • [Journal Article] DsbA directs efficient expression of outer membrane secretin EscC of the enteropathogenic Escherichia coli type III secretion apparatus2008

    • Author(s)
      Miki T., N Okada, Y-S Kim, A Abe, and H Danbara.
    • Journal Title

      Microb.Pathog 44

      Pages: 151-158

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] DsbA directs efficient expression of outer membrane secretin EscC of the enteropathogenic Escherichia coli type III secretion apparatus2008

    • Author(s)
      Miki, T, Okada, N, Kim, Y-S, Abe, A, Danbara, H
    • Journal Title

      Microb Pathog 44

      Pages: 151-158

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] DsbA directs efficient expression of outer membrane secretin EscC of the enterop athogenic Escherichia coli type III secretion apparatus.2008

    • Author(s)
      Miki T, Okada N., Kim Y-S., Abe A., and Danbara H.
    • Journal Title

      Microb.Pathog. 44

      Pages: 151-158

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of amino acid residues of Salmonella SlyA that are critical for transcriptional regulation2007

    • Author(s)
      Okada, N., Y. Oi, M.Takeda-Shitaka, K. Kanou, H. Umeyama, T. Haneda, T. Miki, S. Hosoya and H. Danbara.
    • Journal Title

      Microbiology 153

      Pages: 548-560

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Identification of amino acid residues of Salmonella S1yA that are critical for transcriptional regulation2007

    • Author(s)
      Okada, N, Oi, Y, Takeda-Shitaka, M, Kanou, K, Umeyama, H, Haneda, T, Miki, T, Hosoya, S, Danbara, H
    • Journal Title

      Microbiology 153

      Pages: 548-560

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Identification of amino acid residues of Salmonella SlyA that are critical for transcriptional regulation.2007

    • Author(s)
      Okada, N., Y.Oi, M.Takeda-Shitaka, K.Kanou, H.Umeyama, T.Haneda, T.Miki, S.Hosoya, H.Danbara
    • Journal Title

      Microbiology 153

      Pages: 548-560

    • Related Report
      2006 Annual Research Report
  • [Presentation] Salrvsonellaマクロファージ内増殖に関わるビルレンス因子の同定とその機能解析2008

    • Author(s)
      吉田 雪絵、岡田 信彦、檀原 宏文
    • Organizer
      第81回日本細菌学会総会
    • Place of Presentation
      京都市、京都国際会議場
    • Year and Date
      2008-03-24
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] III型分泌機構を介したSalmonellaプラスミドビルレンスSpvCの分泌2008

    • Author(s)
      清水 啓道、岡田 信彦、檀原 宏文
    • Organizer
      第81回日本細菌学会総会
    • Place of Presentation
      京都市、京都国際会議場
    • Year and Date
      2008-03-24
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Salmonella pathogenicity island2にコードされるSTM1410の機能解析2008

    • Author(s)
      山下 藍、三木 剛志、吉田 雪絵、岡田 信彦、檀原 宏文
    • Organizer
      第81回日本細菌学会総会
    • Place of Presentation
      京都市、京都国際会議場
    • Year and Date
      2008-03-24
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Identification and characterization of Salmonella virulence factors required for bacterial growth in macrophage2008

    • Author(s)
      Yoshida, Y, N, Okada, H, Danbara
    • Organizer
      The 81th annual meeting of the Japanese Society for Microbiology
    • Place of Presentation
      Kyoto
    • Year and Date
      2008-03-24
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Secretion of Salmonella virulence plasmid factor SpvC by the type III secretion system encoded by Salmonella pathogenicity island 12008

    • Author(s)
      Shimizu, M, N, Okada, H, Danbara
    • Organizer
      The 81th annual meeting of the Japanese Society for Microbiology
    • Place of Presentation
      Kyoto
    • Year and Date
      2008-03-24
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Functional analysis of STM1410 encoded by Salmonella pathogenicity island 22008

    • Author(s)
      Yamasita, A, Miki, T, Y, Yoshida, N, Okada, H, Danbara
    • Organizer
      The 81th annual meeting of the Japanese Society for Microbiology
    • Place of Presentation
      Kyoto
    • Year and Date
      2008-03-24
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Salmonellaマクロファージ内増殖に関わるビルレンス因子の同定とその機能解析2008

    • Author(s)
      吉田 雪絵、岡田 信彦、檀原 宏文
    • Organizer
      第81回日本細菌学会総会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2008-03-24
    • Related Report
      2007 Annual Research Report
  • [Presentation] III型分泌機構を介したSalmonellaプラスミドビルレンスSpvCの分泌2008

    • Author(s)
      清水 啓道、岡田 信彦、檀原 宏文
    • Organizer
      第81回日本細菌学会総会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2008-03-24
    • Related Report
      2007 Annual Research Report
  • [Presentation] Salmonella pathogenicity island 2 にコードされるSTMI410の機能解析2008

    • Author(s)
      山下 藍、三木 剛志、吉田 雪絵、岡田 信彦、檀原 宏文
    • Organizer
      第81回日本細菌学会総会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2008-03-24
    • Related Report
      2007 Annual Research Report
  • [Presentation] Regulation of expression of the type III secretion system encoded by Salmonella pathogenicity island 22007

    • Author(s)
      Miki, T, N, Okada, H, Danbara
    • Organizer
      The 89th annual meeting of the Japanese Society for Microbiology in Kanto branch
    • Place of Presentation
      Ikaho, Gunnma
    • Year and Date
      2007-11-16
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] オートファジーによるSalmonella染防御機構の解析2007

    • Author(s)
      福島 直子、岡田 信彦、菊池 雄士、檀原 宏文
    • Organizer
      第20回遺伝子とその周辺研究会
    • Place of Presentation
      大船渡市
    • Year and Date
      2007-08-09
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Salmonellaマクロファージ内増殖に関わるビルレンス因子あ同定とその機能解析2007

    • Author(s)
      吉田 雪絵、羽田 健、岡田 信彦、小寺 義男、大石 正道、前田 忠計、檀原 宏文
    • Organizer
      第20回遺伝子とその周辺研究会
    • Place of Presentation
      大船渡市
    • Year and Date
      2007-08-09
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Chromobacterium violaceumの病原性発現における血型分泌機構の役割2007

    • Author(s)
      井口 美礼、三木 剛志、岡田 信彦、檀原 宏文
    • Organizer
      第20回遺伝子とその周辺研究会
    • Place of Presentation
      大船渡市
    • Year and Date
      2007-08-09
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Autophagy controls by Salmonella infection via innate and acquired immune pathways2007

    • Author(s)
      Fukushima, N, N, Okada, Y, Kikuchi, H, Danbara
    • Organizer
      The 20th Bioscience forum in Kitasato university
    • Place of Presentation
      Ofunato, Iwate
    • Year and Date
      2007-08-09
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Identification and characterization of Salmonella virulence factors required for bacterial growth in Macrophage2007

    • Author(s)
      Yoshida, Y, Haneda, T, N, Okada, Y, Kodera, M, Ohishi, T, Maeda, H, Danbara
    • Organizer
      The 20th Bioscience forum in Kitasato university
    • Place of Presentation
      Ofunato, Iwate
    • Year and Date
      2007-08-09
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Role of the type III secretion systems in pathogenesis of Chromobacterium violaceum2007

    • Author(s)
      Iguchi, M, T, Miki, N, Okada, H, Danbara
    • Organizer
      The 20th Bioscience forum in Kitasato university
    • Place of Presentation
      Ofunato, Iwate
    • Year and Date
      2007-08-09
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The alternative sigma factor RpoN of Listeria monocytogenes contributes to its stationary growth2007

    • Author(s)
      Okada Y, N Okada, S Makino, S Yamamoto, and S Igimi.
    • Organizer
      The 107th General meeting of American Society for Microbiology
    • Place of Presentation
      Canada,Toronto
    • Year and Date
      2007-05-23
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The alterative sigmafactor RpoN of Listeria monocytogenes contributes to its stationary growth2007

    • Author(s)
      Okada, Y, N, Okada, S, Makino, S, Yamamoto, S, Igimi
    • Organizer
      The 107th General meeting of American Society for Microbiology
    • Place of Presentation
      Tronto, Canada
    • Year and Date
      2007-05-23
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Salmonella pathigenicity island2タイプIII分泌装置の機能発現調節2006

    • Author(s)
      三木 剛志、岡田 信彦、檀原 宏文
    • Organizer
      第89回日本細菌学会関東支部総会
    • Place of Presentation
      群馬県伊香保町
    • Year and Date
      2006-11-16
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] サルモネラのアガロース2次元電気泳動マップの作成2006

    • Author(s)
      羽田 健、杉本 茉莉子、岡田 信彦、清水 聡美、行木 乃芙絵、小寺 義男、大石 正道、前田 忠計、檀原 宏文
    • Organizer
      第4回疾患プロテオーム研究会
    • Place of Presentation
      相模原市、北里大学相模原キヤンパス
    • Year and Date
      2006-08-04
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Mapping of Salmonella cell proteins by agarose 2D-electrophoresis2006

    • Author(s)
      Haneda, T, M, Sugimoto, N, Okada, S, Shimizu, N, Nameki, Y, Kodera, M, Ohishi, T, Maeda, H, Danbara
    • Organizer
      The 4th Clinical Proteomics Forum
    • Place of Presentation
      Sagamihara, Kanagawa
    • Year and Date
      2006-08-04
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Salmonella pathigenicity island4の機能解析2006

    • Author(s)
      細野 正人、羽田 健、岡田 信彦、檀原 宏文
    • Organizer
      第19回遺伝子とその周辺研究会
    • Place of Presentation
      相模原市、北里大学相模原キヤンパス
    • Year and Date
      2006-08-02
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Functional analysis of Salmonella pathogenicity 42006

    • Author(s)
      Hosono, M, T, Haneda, N, Okada, H, Danbara
    • Organizer
      The 19th Bioscience Forum in Kitasato university
    • Place of Presentation
      Sagamihara, Kanagawa
    • Year and Date
      2006-08-02
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi