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IMMUNE RESPONSE TO GLYCOLIPIDS SEPARETED FROM MYCOBACTERIUM TUBERCULOSIS

Research Project

Project/Area Number 18590436
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionKitasato University

Principal Investigator

KUMAZAWA Yoshio  Kitasato University, SCHOOL OF SCIENCE, PROFESSOR (30072375)

Co-Investigator(Kenkyū-buntansha) TAKIMOTO Hiroaki  KITASATO UNIV., SCHOOL OF SCIENCE, LECTURER (00253534)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsGuinea pig / mycobacterial glycolipid / Cytokines / IL-12 / IFN-γ / monoclonal antibodies / CD1b
Research Abstract

Chronic inflammation is the hallmark of pathogenesis of tuberculosis and due to the immune responses to not only protein antigens but also various glycolipids produced from Mycobacterium tuberculosis. The mechanisms by which mycobacterial lipid antigens present to T cells via CD1 family, especially CD1b molecule, have been yet unclear. Since guinea pigs carry CD1b molecule, we compared immune responses to protein and glycolipid antigens in guinea pigs. Recombinant guinea pig (gp) IL-12 p70 and IFN-γ were produced by using Sf9 cells transfected with baculo virus AcRP23-LacZ carrying gpIFN-γ, or gpIL-12p40 and gpIL-12p35 genes. We obtained biologically active rgpIFN-γ and rgplL-12 molecules. Using purified these proteins, mice were immunized for preparing monoclonal antibodies (mAb). We got several mAb clones to rgpIFN-γ and rgplL-12 which were able to neutralize the cytokine activy. We established a sandwich ELISA system for the detection and quantitation of IL-12 and IFN-γ.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (10 results)

All 2008 2007 2006 2000 Other

All Journal Article (9 results) (of which Peer Reviewed: 6 results) Presentation (1 results)

  • [Journal Article] Effects of isoflavones and soybeans fermented with Bacillus subtilis on lipopolysaccharide-induced production of tumor necrosis factor α and fibrinolysis in vivo.2007

    • Author(s)
      Hasunuma R, Kawaguchi K, Kikuchi S, Sugiyama T, Kumazawa Y
    • Journal Title

      Immunopharm Immunotox 29

      Pages: 323-333

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunomodulating effects of flavonoids on acute and chronic inflammatory responses caused by tumor necrosis factor α2006

    • Author(s)
      Kumazawa Y.
    • Journal Title

      Cur.Pharm.Design 12

      Pages: 4271-4279

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Involvement of mannose receptor in glycopeptidolipid-mediated inhibition of phagosome-lysosome fusion2006

    • Author(s)
      Shimada K.
    • Journal Title

      Microbiol.Immunol 50

      Pages: 243-251

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Interferon-γ independent formation of pulmonary granuloma in mice by injections with trehalose dimycolate (cord factor),lipoarabinomannan and phosphatidylinositol mannosides isolated from Mycobacterium tuberculosis2006

    • Author(s)
      Takimoto H.
    • Journal Title

      Clin.Exp.Immunol 44

      Pages: 134-141

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Immunomodulating effects of flavonoids on acute and chronic inflammatory responses caused by tumor necrosis factor α2006

    • Author(s)
      Kumazawa, Y
    • Journal Title

      Cur. Pharm. Design 12(32)

      Pages: 4271-4279

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Involvement of mannose receptor in glycopeptidolipid-mediated inhibition of phagosome-lysosome fusion2006

    • Author(s)
      Shimada, K
    • Journal Title

      Microbiol. Immunol 50(3)

      Pages: 243-251

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Interferon-γ independent formation of pulmonary granuloma in mice by injections with trehalose dimycolate (cord factor), lipoarabinomannan and phosphatidylinositol mannosides isolated from Mycobacterium tuberculosis2000

    • Author(s)
      Takimoto, H
    • Journal Title

      Clin. Exp. Immunol 44(1)

      Pages: 134-141

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Quercetin but not luteolin suppresses the induction of lethal shock upon infection of mice with Salmonella typhimurium.

    • Author(s)
      Sugiyama T, Kawaguchi K, Dobashi H, Miyake R, Kaneko M, Kumazawa Y.
    • Journal Title

      FEMS Immunol Microbiol (掲載確定)(in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Suppressive effects of flavonoids quercetin and luteolin on lipid raft accumulation following signal transduction via receptors.

    • Author(s)
      Kaneko M, Takimoto H, Seki Y, Kawaguchi K, Kumazawa Y
    • Journal Title

      Immunopharm Immunotox (掲載確定)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 組換えモルモットサイトカインと単クローン抗体の作製2008

    • Author(s)
      宮崎 弘基、滝本 博明、熊沢 義雄
    • Organizer
      第81回日本細菌学会総会
    • Place of Presentation
      京都
    • Year and Date
      2008-03-24
    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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