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Development of probiotics which regulate bacterial quorum sensing

Research Project

Project/Area Number 18590437
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionKyorin University

Principal Investigator

KAMIYA Shigeru  Kyorin University, Faculty of Medicine, Professor (10177587)

Co-Investigator(Kenkyū-buntansha) OSAKI Takako  Kyorin University, Faculty of Medicine, Instructor (90255406)
HANAWA Tomoko  Kyorin University, Faculty of Medicine, Instructor (80255405)
KURATA Satoshi  Kyorin University, Faculty of Medicine, Instructor (00383670)
田口 晴彦  杏林大学, 医学部, 助教授 (20146541)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,020,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsquorum sensing / probiotics / biofilm / intestinal flora / pathogenesis / クオラムセンシン
Research Abstract

Quorum sensing (QS) is a system for regulation of bacterial gene by autoinducer (AI) which is produced according to bacterial density. Bacterial biofilm is associated with immunological escape, resistance to anti-microbial agents, and its formation is regulated by QS. In order to develop the probiotics which regulate bacterial quorum sensing, the effect of intestinal flora bacteria on biofilm formation by E. coil was examined.
Biofilm assay in microplate was established by using E. coll. Addition of AI-2 increased biofilm formation by E. coli in this assay. Using 16 species and 25 strains of intestinal flora bacteria, it was shown that culture supernatants of Bifidobacterium longm, Clostridium difficile, Enterococcus faecalis, Lactobacillus casei, Peptoniphilus asaccharolyticus and Veillonella parvula inhibited biofilm formation by E. coil. In contrast, culture supernatants of Bcateroides and Fusobacterium necrophorum enhanced biofilm formation. As there was no significant difference in biofilm formation between Helicobacter pylori TK1402 stain and its luxS mutant strain without AI-2 production, it was speculated that biofilm formation was not induced in IL pylori by QS.
Using 576 strains isolated from feces of healthy adults, the effects of culture supernatants of these bacteria on biofilm formation by E. coli was examined. Twelve strains inhibited the biofilm formation with less than 80 % level of control. As biofilm formation by E. coli was not effectively done, the additional experiment using 534 stains was performed. Culture supernatants of 43 and 22 strains inhibited biofilm formation by E. coli by more than 20% and 50%, respectively. Culture supernatants of 42 and 5 strains inhibited biofilm formation by S. aureus by more than 205 and 50%, respectively. Identification of these strains and the effects of these stains on the expression of QS-related genes for adhesin and bacterial toxin need to be clarified.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (37 results)

All 2008 2007 2006

All Journal Article (22 results) (of which Peer Reviewed: 11 results) Presentation (13 results) Book (2 results)

  • [Journal Article] The efficacy of Clostridium butyricum preparation concomitantly with Helicobacter pylori eradication therapy in relation to changer in the intestinal microflora2008

    • Author(s)
      Imase K, Takahashi M, Tanaka A, Tokunaga K, Sugano H, Tanaka M, Ishida H, Kamiya S, Takahashi S
    • Journal Title

      Microbiol Immunol 52(1)

      Pages: 1-6

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Urease-positive bacteria in stomach induce false positive reaction on urea breath test for diagnosis of Helicobacter pylori in fection2008

    • Author(s)
      Osaki T, Mabe K, Hanawa T, Kamiya S
    • Journal Title

      J Med Microbiol (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 腸内フローラ培養上清のBiofilm形成に及ぼす効果2008

    • Author(s)
      宮崎 善史、花輪 智子、大崎 敬子、高橋 秀実、神谷 茂
    • Journal Title

      Bacterial Adherence & Biofilm (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The efficacy of Clostridium butyricum preparation concomitantly with Helicobacter pylori eradication therapy in relation to changes in the intestinal microflora2008

    • Author(s)
      Imase, K., Takahashi, M., Tanaka, A., Tokunaga, K., Sugano, H., Tanaka, M., Ishida, H., Kamiya, S., Takahashi, S
    • Journal Title

      Microbiol Immunol 52 (1)

      Pages: 1-6

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Urease-positive bacteria in stomach induce false positive reaction on urea breath test for diagnosis of Helicobacter pylori infection2008

    • Author(s)
      Osaki, T., Mabe, K., Hanawa, T., Kamiya, S
    • Journal Title

      J Med Microbiol (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effect of culture supernatants of intestinal flora bacteria on biofilm formation (in Japanese)2008

    • Author(s)
      Miyazaki, Y., Hanawa, T., Osaki, T., Takahashi, H., Kamiya, S
    • Journal Title

      Bacterial Adherence & Biofilm (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Urease-positive bacteria in stomach induce false positive reaction on urea breath test for diagnosis of Helicobacter pylori in fection.2008

    • Author(s)
      Osaki T, Mabe K, Hanawa T, Kamiya S
    • Journal Title

      J Med Microbiol (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plasmodium berghei XAT: Contribution of gammadelta T cells to host defense against infection with blood-stage nonlethal malari a parasite2007

    • Author(s)
      Kobayashi F, Niikura M, Waki S, Matsui T, Fujino T, Tsur uhara T, Kamiya S
    • Journal Title

      Exp Parasitol 117(4)

      Pages: 368-375

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Antifungal drug susceptibility of Cryptococcus neoformans from clinical sources in Nairobi,Kenya2007

    • Author(s)
      Bii CC, Makimura K, Abe S, Taguchi H Mugasia OM Revathi G, Wamae NC, Kamiya S
    • Journal Title

      Mycoses 50(1)

      Pages: 25-30

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Plasmodium berghei XAT : Contribution of gammadelta T cells to host defense against infection with blood-stage nonlethal malaria parasite2007

    • Author(s)
      Kobayashi, F., Niikura, M., Waki, S., Matsui, T., Fujino, T., Tsuruhara, T., Kamiya, S
    • Journal Title

      Exp Parasitol 117 (4)

      Pages: 368-375

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Antifungal drug susceptibility of Cryptococcus neoformans from clinical sources in Nairobi, Kenya2007

    • Author(s)
      Bii, CC., Makimura, K., Abe, S., Taguchi, H., Mugasia, OM., Revathi, G., Wamae, NC., Kamiya, S
    • Journal Title

      Mycoses 50 (1)

      Pages: 25-30

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Plasmodium berghei XAT:Contribution of gammadelta T cells to host defense against infection with blood-stage nonlethal malari a parasite.2007

    • Author(s)
      Kobayashi F, Niikura M, Waki S, Matsui T, Fujino T, Tsuruhara T, Kamiya S
    • Journal Title

      Exp Parasitol. 117(4)

      Pages: 368-375

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antifungal drug susceptibility of Cryptococcus neoformans from clinical sources in Nairobi,2007

    • Author(s)
      Bii OC, Makimura K, Abe S, Taguchi H, Mugasia OM, Revathi G, Wamae NC, Kamiya S
    • Journal Title

      Kenya. Mycoses 50(1)

      Pages: 25-30

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mutation of luxS affects motility and infectivity of Helicobact er pylori in gastric mucosa of Mongolian gerbil model2006

    • Author(s)
      Osaki T, Hanawa T, Manzoku T, Fukuda M, Kawakami H, Suzuki H, Yamaguchi H, Yen X, Taguchi H, Kurata S, Kamiya S
    • Journal Title

      J Med Microbiol 55(11)

      Pages: 1477-1485

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] In vitro antibacterial activity of a novel antimicrobial agent TG44 for treatment of Helicobacten pylori2006

    • Author(s)
      Kamoda 0, Anzai K, Mizoguchi J, Yanagi T, Shioiri M, Nishio T. Kamiva S
    • Journal Title

      Antimicrob Agents Chemother 50(9)

      Pages: 3062-3069

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] プロバイオティクスの臨床応用-腸管感染症-2006

    • Author(s)
      田口 晴彦、高橋 志達、神谷 茂
    • Journal Title

      臨床と微生物 33(2)

      Pages: 153-157

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Mutation of luxS affects motility and infectivity of Helicobacter pylori in gastric mucosa of Mongolian gerbil model2006

    • Author(s)
      Osaki, T., Hanawa, T., Manzoku, T., Fukuda, M., Kawakami, H., Suzuki, H., Yamaguchi, H., Yen, X., Taguchi, H., Kurata, S., Kamiya, S
    • Journal Title

      J Med Microbiol 55 (11)

      Pages: 1477-1485

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] In vitro antibacterial activity of a novel antimicrobial agent TG44 for treatment of Helicobacter pylori2006

    • Author(s)
      Kamoda, O., Anzai, K., Mizoguchi, J., Yanagi, T., Shioiri, M., Nishio, T., Kamiya, S
    • Journal Title

      Antimicrob Agents Chemother 50 (9)

      Pages: 3062-3069

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Clinical use of probiotics- intestinal infectious diseases (in Japanese)2006

    • Author(s)
      Taguchi, H., Takahashi, M., Kamiya, S
    • Journal Title

      Rinsho to Biseibutsu 33 (2)

      Pages: 153-157

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Mutation of luxS affects motility and infectivity of Helicobacter pylori in gastric mucosa of Mongolian gerbil model.2006

    • Author(s)
      Osaki T, Hanawa T, Manzoku T, Fukuda M, Kawakami H, Suzuki H, Yamaguchi H, Yen X, Taguchi H, Kurata S, Kamiya S
    • Journal Title

      J Med Microbiol 55(11)

      Pages: 1477-1485

    • Related Report
      2006 Annual Research Report
  • [Journal Article] In vitro antibacterial activity of a novel antimicrobial agent TG44 for treatment of Helicobacter pylori.2006

    • Author(s)
      Kamoda O, Anzai K, Mizoguchi J, Yanagi T, Shioiri M, Nishio T, Kamiya S
    • Journal Title

      Antimicrob Agents Chemother 50(9)

      Pages: 3062-3069

    • Related Report
      2006 Annual Research Report
  • [Journal Article] プロバイオティクスの臨床応用-腸管感染症-2006

    • Author(s)
      田口晴彦, 高橋志達, 神谷茂
    • Journal Title

      臨床と微生物 33(2)

      Pages: 153-157

    • Related Report
      2006 Annual Research Report
  • [Presentation] Helicobacter pyloriのバイオフィルム形成能に関する基礎的研究2008

    • Author(s)
      神谷 茂、米澤 英雄、蔵田 訓、花輪 智子、大崎 敬子
    • Organizer
      第12回日本小児H.pylori研究会
    • Place of Presentation
      松本市
    • Year and Date
      2008-03-08
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Basic study on biofilm forming ability of Helicobacter pylori strains2008

    • Author(s)
      Kamiya, S., Yonezawa, H., Kurata, S., Hanawa, T., Osaki, T
    • Organizer
      The 12th Annual Meeting of Japanese Pediatric Society for H. pylori Research
    • Place of Presentation
      Matsumoto
    • Year and Date
      2008-03-08
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Characterization of in vitro Biofilm Formation by Helicobacter pylori2008

    • Author(s)
      Yonezawa H, Osaki T, Kurata S, Hanawa T, Kamiya S
    • Organizer
      7th China-Korea-Japan Joint Conference on Helicobacter Infection
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Characterization of in vitro biofilm formation by Helicobacter pylori2008

    • Author(s)
      Yonezawa, H., Osaki, T., Kurata, S., Hanawa, T., Kamiya, S
    • Organizer
      The 7th China-Korea-Japan Joint Conference on Helicobacter Infection
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Characterzation of in vitro Biofilm Formation by Helicobacter pylori2008

    • Author(s)
      Yonezawa H, Osaki T, Kurata S, Hanawa T, Kamiya S
    • Organizer
      7^<th> China-Korea-Japan Joint Conference on Helico batter Infection.
    • Place of Presentation
      Kyoto
    • Related Report
      2007 Annual Research Report
  • [Presentation] Helicobacter pylori TK1402株バイオフィルム形成の解析2007

    • Author(s)
      米澤 英雄、大崎 敬子、蔵田 訓、花輪 智子、神谷 茂
    • Organizer
      第36回杏林医学会総会
    • Place of Presentation
      三鷹市
    • Year and Date
      2007-11-17
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Analysis of biofilm formation by Helicobacter pylori TK1402 strain2007

    • Author(s)
      Yonezawa, H., Osaki, T., Kurata, S., Hanawa, T., Kamiya, S
    • Organizer
      The 36th Annual Meeting of Kyorin Medical Society
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-11-17
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 腸内フローラ培養上清のBiofilm形成に及ぼす効果2007

    • Author(s)
      宮崎 善史、花輪 智子、大崎 敬子、高橋 秀実、神谷 茂
    • Organizer
      Bacterial Adherence&Biofilm第21回学術集会
    • Place of Presentation
      東京都
    • Year and Date
      2007-07-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effect of culture supernatants of intestinal flora on bacterial biofilm formation The 21st Annual Meeting of Bacterial Adherence and Biofilm2007

    • Author(s)
      Miyazaki, Y., Hanawa, T., Osaki, T., Takahashi, H., Kamiya, S
    • Organizer
      The 21st Annual Meeting of Bacterial Adherence and Biofilm
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-07-07
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 腸内フローラ培養上清のBiofilm形成に及ぼす効果2007

    • Author(s)
      宮崎 善史、花輪 智子、大崎 敬子、高橋 秀実、神谷 茂
    • Organizer
      Bacterial Adherence & Biofilm 第21回学術集会
    • Place of Presentation
      東京都
    • Year and Date
      2007-07-07
    • Related Report
      2007 Annual Research Report
  • [Presentation] ハムスターClostridium difficile関連疾患モデルの作成とプロバイオティクスの有効性に関する研究2007

    • Author(s)
      田口 晴彦、蔵田 訓、中西 崇介、高橋 志達、神谷 茂
    • Organizer
      第81回日本感染症学会総会
    • Place of Presentation
      京都市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Establishment of hamster model for Clostridium difficile-associated diseases (CDAD) and studies on the efficiency of probiotics on CDAD2007

    • Author(s)
      Taguchi, H., Kurata, S., Nakanishi, S., Takahashi, M., Kamiya, S
    • Organizer
      The 81st Annual Meeting of Japanese Society for Infectious Diseases
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ハムスターClostridium diffcile関連疾患モデルの作成とプロバイオティクスの有効性に関する研究2007

    • Author(s)
      田口 晴彦、蔵田 訓、中西 崇介、高橋 志達、神谷 茂
    • Organizer
      第81回日本感染症学会総会
    • Place of Presentation
      京都市
    • Related Report
      2007 Annual Research Report
  • [Book] アンチエイジング・ヘルスフード-抗加齢、疾病予防、健康長寿延長への応用2008

    • Author(s)
      神谷 茂
    • Total Pages
      463
    • Publisher
      サイエンスフォーラム社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] アンチエイジング・ヘルスフード-抗加齢・疾病予防・健康長寿延長への応用-2008

    • Author(s)
      神谷 茂
    • Total Pages
      463
    • Publisher
      サイエンスフォーラム社
    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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