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Strategy of paramyxovirusto attenuate cytopathicity

Research Project

Project/Area Number 18590447
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionMie University

Principal Investigator

TSURUDOME Masato  Mie University, Graduate School of Medicne, Associate Professor (50159042)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsparamyxovirus / SV5 / cell-cell fusion / cytopathicity / multinucleated giant cell / HN protein / F protein / receptor
Research Abstract

Paramyxoviruses including simian virus 5(SV5)mediate membrane fusion through an interaction between viral envelope glycoproteins HN and F, in which the HN protein promotes fusion-inducing function of the F protein. I report here that the stalk region of SV5 HN protein is involved in the interaction with the F protein the same as those of other paramyxoviruses. Furthermore, I report for the first time that four discrete domains of the F proteins participate in the interaction with the FIN protein. As published previously, on the other hand, the T1 strain of SV5 displays reduced ability to induce cell-cell fusion and thus brings minimal cytopathicity to the virus-infected cells as compared to the prototype WR strain. To investigate the molecular mechanism of the attenuated cytopathicity of T1 virus, highly fusogenic variant was obtained from T1 virus by plaque cloning. This variant possessed three mutations L182R, K45 IT, and V536M in the FIN protein, with no mutation in the F protein. Notably, L182R mutation proved to raise the fusion-promoting function of the HN protein and that mutation of leucine at position 182 to other amino acids other than Ala also facilitates the fusion-promoting function of the HN protein. However, either of the recombinant WR viruses whose HN and/or F proteins had been replaced with the Ti counterparts were fusogenic, indicating that other T1 virus proteins such as M protein might contribute to the fusion-inhibiting function of the T1 FIN protein. Since T1 virus could perform multiple-step replication, the fusion between envelope and cell membrane seems to take place normally. We thus hypothesize that T1 FIN protein may suppresses cell-cell fusion by transducing a signal to the intracellular molecules such as actin or vinculin that the L182R mutation or combination with WR M protein may somehow cancel this suppression of signal transduction.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (20 results)

All 2007 2006

All Journal Article (16 results) (of which Peer Reviewed: 5 results) Presentation (4 results)

  • [Journal Article] Failure of multinucleated giant cell formation in k562 cells infected with newcastle disease virus and human parainfluenza type 2 virus.2007

    • Author(s)
      Izumi Yamakawa
    • Journal Title

      Microbiology and Immunology 51

      Pages: 601-608

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Existence of leptin receptor protein in chicken tissues: isolation of a monoclonal antibody against chicken leptin receptor.2007

    • Author(s)
      Takeshi Ohkubo
    • Journal Title

      General and Comparative Endocrinology 151

      Pages: 269-273

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The conserved carboxyl terminus of human parainfluenza virus type 2 V protein plays an important role in virus growth.2007

    • Author(s)
      Machiko Nishio
    • Journal Title

      Virology 362

      Pages: 85-98

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The conserved carboxyl terminus of human parainfluenza virus type 2 V protein plays an important role in virus growth2007

    • Author(s)
      Nishio, M., Tsurudome, M., Ishihara, H., Ito, M., Ito, Y
    • Journal Title

      Virology 362(1)

      Pages: 85-98

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Existence of leptin receptor protein in chicken tissues : Isolation of a monoclonal antibody against chicken leptin receptor2007

    • Author(s)
      Ohkubo, T., Nishio, M., Tsurudome, M., Ito, M., Ito, Y
    • Journal Title

      General and Comparative Endocrinology 151(3)

      Pages: 269-273

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Failure of multinucleated giant cell formation in k562 cells infected with Newcastle disease virus and human parainfluenza type 2 virus2007

    • Author(s)
      Yamakawa, I., Tsurudome, M., Kawano, M., Nishio, M., Komada, H., Ito, M., Uji, Y., Ito, Y
    • Journal Title

      Microbiology and Immunology 51(6)

      Pages: 601-608

    • NAID

      10020280000

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Failure of multinucleated giant cell form ation in K562 cells infected with Newcastle disease virus and human parainfluenza type 2 virus.2007

    • Author(s)
      Izumi Yamakawa
    • Journal Title

      Microbiology and Immunology 51

      Pages: 601-608

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Existence of leptin receptor protein in chicken tissues: isolation of a monoclonal antibody against chicken leptin receptor2007

    • Author(s)
      Takeshi Ohkubo
    • Journal Title

      General and Comparative Endocrinology 151

      Pages: 269-273

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A mutant fusion (F) protein of simian virus 5 induces hemagglutinin-neuraminidase-independent syncytium formation despite the internalization of the F protein2006

    • Author(s)
      Tsurudome, M., Ito, M., Nishio, M., Kawano, M., Komada, H., Ito, Y
    • Journal Title

      Virology 347(1)

      Pages: 11-27

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The properties of recombinant Sendai virus having the P gene of Sendai virus pi strain derived from BEM cells persistently infected with Sendai virus2006

    • Author(s)
      Nishio, M., Nagata, A., Yamamoto, A., Tsurudome, M., Ito, M., Kawano, M., Komada, H., Ito, Y
    • Journal Title

      Medical Microbiology and Immunology 195(3)

      Pages: 151-158

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Possible activation of murine T lymphocyte through CD98 is independent of interleukin 2/interleukin 2 receptor system2006

    • Author(s)
      Komada, H., Imai, A., Hattori, E., Ito, M., Tsumura, H., Onoda, T., Kuramochi, M., Tani, M., Yamamoto, K., Yamane, M., Kawano, M., Nishio, M., Yuasa, K., O'Brien, M., Yamamoto, H., Uematsu, J., Tsurudome, M., Ito, Y
    • Journal Title

      Biomedical Research 27(2)

      Pages: 61-67

    • NAID

      130004903708

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Defense mechanism against respiratory infection2006

    • Author(s)
      Tsurudome, M., Ito, Y
    • Journal Title

      Nippon Rinsho 64(10)

      Pages: 1808-1812

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Identification of RNA-binding regions on the P and V proteins of human parainfluenza virus type 2.2006

    • Author(s)
      Machiko Nishio
    • Journal Title

      Medical Microbiology and Immunology 195・1

      Pages: 29-36

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The properties of recombinant Sendai virus having the P gene of Sendai virus pistrain derived from BHK cells persistently infected with Sendai virus.2006

    • Author(s)
      Machiko Nishio
    • Journal Title

      Medical Microbiology and Immunology 195・3

      Pages: 1-3

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Possible activation of murine T lymphocyte through CD98 is independent of interleukin 2/interleukin 2 receptor system.2006

    • Author(s)
      Komada Hiroshi
    • Journal Title

      Biomedical Research 27・2

      Pages: 61-67

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A mutant fusion (F) protein of simian virus 5 induces hemagglutinin-neuraminidase-independent syncytium formation despite the internalization of the F protein.2006

    • Author(s)
      Masato Tsurudome
    • Journal Title

      Virology 347・1

      Pages: 11-27

    • Related Report
      2006 Annual Research Report
  • [Presentation] Mapping of the domains on the paramyxovirus fusion protein that determine hemagglutinin-neuraminidase specificity in mediating syncytium formation.2007

    • Author(s)
      Masato Tsurudome
    • Organizer
      Workshop on Replication and Cell Biology of Negative Strand RNA Viruses
    • Place of Presentation
      エヴァンストン,イリノイ州アメリカ合衆国
    • Year and Date
      2007-09-16
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Mapping of the domains on the paramyxovirus fusion protein that determine hemagglutinin-neuraminidase specificity in mediating syncytium formation2007

    • Author(s)
      Tsurudome, M., Nishio, M., Ohtsuka, J., Kawano, M
    • Organizer
      Workshop on Replication and Cell Biology of Negative Strand RNA Viruses
    • Place of Presentation
      Evanston, Illinois, U.S.A.
    • Year and Date
      2007-09-16
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Mapping of the domains on the paramyxovirus fusion protein that determine hemalutinin-neuraminidase secificity in mediatin snctium formation2007

    • Author(s)
      Masato Tsurudome
    • Organizer
      Workshop on Replication and Cell Biology of Negative Strand RNA Viruses
    • Place of Presentation
      エヴァンストン,イリノイ州,アメリカ合衆国
    • Year and Date
      2007-09-16
    • Related Report
      2007 Annual Research Report
  • [Presentation] Conserved carboxyl terminal of human parainfluenza virus type 2 V protein plays an important role in virus growth2006

    • Author(s)
      Nishio, M., Tsurudome, M., Ito, M., Ito, Y
    • Organizer
      XIII International Conference on Negative Strand Viruses
    • Place of Presentation
      Salamanca, Spain
    • Year and Date
      2006-06-19
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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