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A research for immune regulation using ES cells

Research Project

Project/Area Number 18590478
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Immunology
Research InstitutionSt.Marianna University School of Medicine

Principal Investigator

SEINO Ken-ichiro  St.Marianna University School of Medicine, Institute of Medical Science, Associate Professor (20312845)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,020,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsNKT cells / dendritic cells / cell differentiation / 細胞分代
Research Abstract

NKT cells are an immune regulatory cells bridging the innate and acquired immune systems. It has been expected that one can manipulate NKT cells well to better regulate immune system for a management of intractable diseases. In this study, we have investigated whether it is possible to generate dendritic cells or NKT cells from immature cells such as ES cells.
We generated DCs in vitro by using OP9 cells and GM-CSF. ES cell-derived DCs expressed CD11c or CD80/86 and capable to stimulate NKT cells when pulsed with a-GalCer. In the presence of IL-10, the NKT cells and DCs lost the ability of producing inflammatory cytokines but obtained that of IL-10, one of immune regulatory cytokines.
In this study, we found that mutant of iPLA2 induced increase of the frequency of NKT cells in vivo. iPLA2 inhibitor, BEL, induced the increase of NKT cells in FTOC. It was very difficult to induce the differentiation of NKT cells in vitro from hematopoietic stem cells or fetal liver cells, but it was expected the inhibition of iPLA2 could improve the ratio of NKT cell production from the progenitor cells.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (13 results)

All 2007 2006

All Journal Article (11 results) (of which Peer Reviewed: 4 results) Presentation (2 results)

  • [Journal Article] Differential role of TSLP in the induction of airway hyperreactivity and Th2 immune response in antigen-induced asthma with respect to NKT cell function2007

    • Author(s)
      Nagata Y, et. al.
    • Journal Title

      Int Arch Allerg and Immunol 144

      Pages: 305-314

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Mechanism of NKT cell-mediated transplant tolerance2007

    • Author(s)
      Jiang X, et. al.
    • Journal Title

      Am J Transplant 7

      Pages: 1482-1490

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Critical Role for CXC Chemokine Ligand 16(SR-PSOX) in Th1 Response Mediated by NKT Cells2007

    • Author(s)
      Shimaoka T, et. al.
    • Journal Title

      J Immunol 179

      Pages: 8172-8179

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Differential role of TSLP in the induction of airway hyperreactivity and Th2 immune resopnse in antigen-induced asthma with respect to NKT cell function2007

    • Author(s)
      Nagata Y
    • Journal Title

      Int Arch Allerg and Immunol 144

      Pages: 305-314

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Mechanism of NKT cell-mediated transplant tolerance.2007

    • Author(s)
      Jiang X, Kojo s, Harada M, Ohkohchi N, Taniguchi M, Seino K
    • Journal Title

      Am J Transplant 7

      Pages: 1482-1490

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Critical Role for CXC Chemokine Ligand 16(SR-PSOX) in Th1 Response Mediated by NKT Cells.2007

    • Author(s)
      Shimaoka T, Seino K, Kume N, Minami M, Nishime C, Suematsu M, Kita T, Taniguchi M, Matsushima K, Yonehara S.
    • Journal Title

      J Immunol 179

      Pages: 8172-9

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Critical Role for CXC Chemokine Ligand 16(SR-PSOX)in Th1 Response Mediated by NKT Cells2007

    • Author(s)
      Shimaoka T, et. al.
    • Journal Title

      J Immunol 179

      Pages: 8172-8179

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulatory dendritic cells act as regulators of acute lethal systemic inflammatory response.2006

    • Author(s)
      Fujita, S., et. al.
    • Journal Title

      Blood 107

      Pages: 3656-3664

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The importance of CD25^+ CD4^+ regulatory T cells in mouse hepaticallograft tolerance.2006

    • Author(s)
      Xiaofeng Jiang, et. al.
    • Journal Title

      Liver Transplantation 12

      Pages: 1112-1118

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Natural killer T cell-mediated antitumor immune responses and their clinical applications.2006

    • Author(s)
      Seino K, et. al.
    • Journal Title

      Cancer Sci. 92

      Pages: 807-812

    • NAID

      10018767026

    • Related Report
      2006 Annual Research Report
  • [Journal Article] IL-21-induced B ε cell apoptosis mediated by natural killer T cells suppresses IgE responses.2006

    • Author(s)
      Michishige Harada, et. al.
    • Journal Title

      Journal of Experimental medicine VOL203

      Pages: 2929-2937

    • Related Report
      2006 Annual Research Report
  • [Presentation] International of NKT cells and DCs in nasal vaccination2007

    • Author(s)
      清野 研一郎
    • Organizer
      日本免疫学会総会
    • Place of Presentation
      東京
    • Year and Date
      2007-11-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] International of NKT cells and DCs in nasal vaccination2007

    • Organizer
      International Congress of Immunology
    • Place of Presentation
      Tokyo Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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