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Clarification of LIM protein family-mediated system that regulates innate immune responses.

Research Project

Project/Area Number 18590480
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Immunology
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

TANAKA Takashi  The Institute of Physical and Chemical Research, Host Defense Laboratory, Researcher (00415225)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,020,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsLIM proteins / innate immunity / ubiouitin E3 Haase / NF-κB / 樹状細胞
Research Abstract

PDLIM2 is a nuclear LIM protein that binds to and inhibits the activity of STAT4 transcription factor. In this study, we have demonstrated that PDLIM2 also negatively regulates the activity of NF-kB transcription factor, acting as a nuclear ubiquitin E3 ligase toward the p65 subunit of NF-KB (Tanaka, et. al., Nat. Immuno1. 8, 584, 2007). PDLIM2 binds to p65 and promotes p65 polyubiquitination through its LIM domain. In addition, PDLIM2 targets p65 to discrete intranuclear compartments called PML nuclear bodies. PML nuclear bodies are nuclear proteolytic centers where proteasomal components are concentrated. Polyubiquitinated p65 is ultimately degraded by proteasome in this compartment. A PDLIM2 mutant lacking the PDZ domain fails to target p65 to nuclear bodies, suggesting that PDLIM2 mediates intranuclear trafficking of p65 through its PDZ domain. PDLIM2 deficiency results in larger amounts of nuclear p65, defective p65 ubiquitination and augmented production of proinflammatory cytokines in response to TLR ligands, such as LPS and CpG-DNA. These findings delineate a novel pathway by which PDLIM2 terminates NF-kB activation through intranuclear sequestration and subsequent degradation. Moreover, we have shown that PDLIM2 can also inhibit both IRF3 and IRF7-mediated transactivation in luciferase assay with IFNIβ or IFNα□promoter-driven reporter construct, respectively. Among LIM protein family, Ril (PDLIM4), elfin (PDLIM1) and ALP (PDLIM3) are the most closely related with PDLIM2Moreover, we have found that these LIM proteins could inhibit NF-KB-mediated transactivation in luciferase assay with reporter containing NF-kB binding site. These findings suggest that LIM proteins are the novel family that negatively regulates signal transductions in immune system.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (27 results)

All 2008 2007 2006 Other

All Journal Article (13 results) (of which Peer Reviewed: 7 results) Presentation (10 results) Remarks (1 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] PDLIM2-mediated termination of transcription factor NF-κB activation by intranuclear sequestration and degradation of the p65 subunit.2007

    • Author(s)
      T. Tanaka
    • Journal Title

      Nature Immunol. 8

      Pages: 584-591

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The C/EBPβ Isoform 24-kDa LAP is responsible for NF-IL-6-mediated gene induction in activated macrophsges, but is not essential for intracellular bacteria killing.2007

    • Author(s)
      S. Uematsu
    • Journal Title

      J. Immunol. 179

      Pages: 5378-5386

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Regulation of Signal Transducer and Activator of Transcription Signaling by the Tyrosine Phosphatase PTP-BL.2007

    • Author(s)
      M. Nakahira
    • Journal Title

      Immunity 26

      Pages: 163-176

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] PDLIM2-mediated termination of transcription factor NF-kB activation by intranuclear sequestration and degradation fo the p65 subunit.2007

    • Author(s)
      T. Tanaka
    • Journal Title

      Nature Immunol. 8(6)

      Pages: 584-591

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Regulation of Signal Transducer and Activator of Transcription Signaling by the Tyrosine Phosphatase2007

    • Author(s)
      M. Nakahira
    • Journal Title

      Immunity. 26

      Pages: 163-176

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] PDLIM2-mediated termination of transcription factor NF-κB activation by intranuclear sequestration and degradation of the p65 subunit.2007

    • Author(s)
      Takashi Tanaka
    • Journal Title

      Nature Immunology 8

      Pages: 584-591

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The C/EBPβ isoform 34kDa LAP is responsible for NF-IL6-mediated gene induction in activated macrophages,but is not essential for intracellular bacteria killing.2007

    • Author(s)
      Satoshi Uematsu
    • Journal Title

      Journal of Immunology 179

      Pages: 5378-5386

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of signal transducer and activator of transcription signaling by the tyrosine phosphatase PTP-BL.2007

    • Author(s)
      Masakiyo Nakahira
    • Journal Title

      Immunity (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] IκB kinase-α is critical for interferon-α production induced by Toll-like receptors 7 and 9.2006

    • Author(s)
      K. Hoshino
    • Journal Title

      Nature 440

      Pages: 949-953

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Gene expression profiles of mouse dendritic cell subsets.2006

    • Author(s)
      M. Saito
    • Journal Title

      J. Tokyo Med. Univ. 64

      Pages: 294-304

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] IkB Kinaseα is critical for interferon α production induced by Toll-like receptors7 and 9.2006

    • Author(s)
      K. Hoshino
    • Journal Title

      Nature. 440

      Pages: 949-953

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Gene expression profiles of mouse dendritic cell subsets2006

    • Author(s)
      M. Saito
    • Journal Title

      J. TokyoMed. Univ. 64(3)

      Pages: 294-304

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] IκB kinase-αis critical for interferon-α production induced by Toll-like receptors 7 and 9.2006

    • Author(s)
      Katsuaki Hoshino
    • Journal Title

      Nature 440

      Pages: 949-953

    • Related Report
      2006 Annual Research Report
  • [Presentation] HSP70 terminates NF-κB activation by facilitating degradation of NF-κB p65 subunit.2008

    • Author(s)
      T. Tanaka
    • Organizer
      Keystone Symposia
    • Place of Presentation
      Colorado, USA
    • Year and Date
      2008-02-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] HSP70 terminates NF-kB activation by facilitating degradation of NF-kB p65 subunit.2008

    • Author(s)
      T. Tanaka
    • Organizer
      Keystone Symposia
    • Place of Presentation
      Colorado, USA
    • Year and Date
      2008-02-27
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Heat shock proteins regulate PDLIM2-mediated termination of NF-κB activation.2007

    • Author(s)
      T. Tanaka
    • Organizer
      The 37th Annual Meeting of the Japanese Society for Immunology
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2007-11-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Heat shock proteins regulate PDLIM2-mediated termination of NF-kB activation.2007

    • Author(s)
      T. Tanaka
    • Organizer
      The 37th Annual Meeting of the Japanese Society for Immunology
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2007-11-20
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] PDLIM2依存性NF-κB不活性化機構は熱ショックタンパク質群によって制御される。2007

    • Author(s)
      田中 貴志
    • Organizer
      日本免疫学会
    • Place of Presentation
      東京
    • Year and Date
      2007-11-20
    • Related Report
      2007 Annual Research Report
  • [Presentation] Heat shock proteins regulate PDLIM2-mediated NF-κB activation.2007

    • Author(s)
      T. Tanaka
    • Organizer
      20th Naito conference on Innate immunity in medicine and biology.
    • Place of Presentation
      Kanagawa, Japan
    • Year and Date
      2007-10-11
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Heat shock proteins regulate PDLIM2-mediated NF-kB2007

    • Author(s)
      T. Tanaka
    • Organizer
      20th Naito conference on Innate immunity in medicine and biology
    • Place of Presentation
      Kanagawa, Japan
    • Year and Date
      2007-10-11
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Heat shock proteins regulate PDLIM2-mediated NF-κB activation.2007

    • Author(s)
      田中 貴志
    • Organizer
      20^<th>NAITO conference on Innate immunity in medicine and biology
    • Place of Presentation
      湘南、神奈川
    • Year and Date
      2007-10-11
    • Related Report
      2007 Annual Research Report
  • [Presentation] SLIM, a nuclear ubiquitin E3 ligase, terminates NF-κB activation by itssequestration and degradation.2006

    • Author(s)
      T. Tanaka
    • Organizer
      The 36th Annual Meeting of the Japanese Society for Immunology
    • Place of Presentation
      Osaka, Japan
    • Year and Date
      2006-12-13
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] SLIM, a nuclear ubiquitin E3 ligase, terminates NF-kB activation by its sequestration and degiadation2006

    • Author(s)
      T. Tanaka
    • Organizer
      The 36th Annual Meeting of the Japanese Society for Immunology
    • Place of Presentation
      Osaka, Japan
    • Year and Date
      2006-12-13
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://web.rcai.riken.jp/en/labo/host/

    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] p65媒介シグナル伝達の調節剤およびスクリーニング方法2007

    • Inventor(s)
      田中貴志, 改正恒康, Michael J Grusby
    • Industrial Property Rights Holder
      独立行政法人理化学研究所, President and Fellowsof HarvardCollege
    • Filing Date
      2007-03-15
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] p65媒介シグナル伝達の調節剤およびそのスクリーニング方法2007

    • Inventor(s)
      田中 貴志 改正 恒康Michael J Grusby
    • Industrial Property Rights Holder
      理化学研究所
    • Filing Date
      2007-03-15
    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] p65媒介シグナル伝達の調節剤およびそのスクリーニング方法2006

    • Inventor(s)
      田中貴志
    • Industrial Property Rights Holder
      独立行政法人理化学研究所
    • Filing Date
      2006
    • Related Report
      2006 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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