The change of lysophospholipid balance in the lipoprotein is a new biomarker for the coronary artery disease

• Project/Area Number: 18590521
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Gunma University
• Principal Investigator: KUWABARA Atsushi Gunma University, 医学部, 助教 (90323344)
• Co-Investigator(Kenkyū-buntansha): MURAKAMI Masami 群馬大学, 大学院・医学系研究科, 教授 (30241871) ; OKAJIMA Fumikazu 群馬大学, 生体調節研究所, 教授 (30142748)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥660,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: スフィンゴシン1リン酸 / リゾフォスファチジン酸 / リポ蛋白 / 動脈硬化 / スフィンゴシン1-リン酸 / 冠動脈疾患

Research Abstract

冠動脈疾患患者のLDL中に含まれるスフィンゴシン1-リン酸(S1P)とリゾフォスファチジン酸(LPA)の量を測定し、また、LDLによる血管平滑筋細胞の遊走反応を調べ、脂質分子バランスと平滑筋細胞の遊走反応との関係を検討した。基礎的検討において、LPAはLPA1受容体を介して血管平滑筋細胞の遊走活性を促進し、S1PはS1P2受容体を介して血管平滑筋細胞の遊走活性を抑制することを見出した。

Research Products

• [Journal Article] LPA1 receptors mediate stimulation, whereas LPA2 receptors mediate inhibitionof migration of pancreatic cancer cells in response tolysophosphatidic acid and malignant ascites (2009)
  • Author(s): Komachi M, Tomura H, Malchinkhuu E, Tobo M, Mogi C, Yamada T, Kimura T, Kuwabara A, Ohta H, Im DS, Kurose H, TK, Okajima F
  • Journal Title: Carcinogenesis 30(3) Pages: 457-65
  • Peer Reviewed
• [Journal Article] LPA1 receptors mediate stimulation, whereas LPA2 receptors mediate inhibition, of migration of pancreatic cancer cells in response to lysophosphatidic acid and malignant ascites. (2009)
  • Author(s): Komachi M, Tomura H, Malchinkhuu E, Tobo M, Mogi C, Yamada T, Kimura T, Kuwabara A Ohta H, Im DS, Kurose H, Takevoshi I, SatoK. Okaiima F.
  • Journal Title: Carcinogenesis. 30(3 ) Pages: 457-65
  • Peer Reviewed
• [Journal Article] Induction of scavenger receptor class B type I is critical for simvastatin enhancement of high-density lipoprotein-induced anti-inflammatory actions in endothelial cells (2008)
  • Author(s): Kimura T, Mogi C, Tomura H,Kuwabara A, Im DS, Sato K, Kurose H, Murakami M, Okajima F
  • Journal Title: J Immounol Nov15 181(10) Pages: 7332-40
  • Peer Reviewed
• [Journal Article] Induction of scavenger receptor class B tylpe I is critical for simvastat in enhancement of high-density lipoprotein-induced anti-inflammatory actions in endothelial cells. (2008)
  • Author(s): Kimura T, Mogi C, Tomura H, Kuwabara A, Im DS, Sato K, Kurose H, Murakami M, Okajima F.
  • Journal Title: J Immunology 181(10) Pages: 7332-40
  • Peer Reviewed
• [Journal Article] Critical role of ABCA1 transporter in sphingsine 1-phosphate release from astrocytes (2007)
  • Author(s): Sato K, Malchinkhuu E, HoriuchiY, Moji C, Trmura H Tosake M
  • Journal Title: J Neurchem 103(6) Pages: 2610-19
  • Peer Reviewed
• [Journal Article] HDL-like lipoproteins in cerebrospinal fluid affeneural cell activity throughlipoprotein miqratory associated sphingosine 1-phosphate (2007)
  • Author(s): Sato K, Malchinkhuu E, Horiuchi Y, Mogi Tomura H, Tosaka M, Yoshimoto Y, Kuwabara A, Okajima F
  • Journal Title: Biochem Biophys Res Commun 3 ; 359(3) Pages: 649-54
  • Peer Reviewed
• [Journal Article] Role of lipoprotein-associated lysophospholipids in mactivity of coronary artery smooth muscle cells (2007)
  • Author(s): Damirin A, Tomura H, Komachi M, Liu JP, Mogi C, Tobo M, Wang JQ, Kimura T, Kuwabara A, Yamazaki Y, Ohta H, Im DS, Sato K, Okajima F
  • Journal Title: Am J Physiol Heart Cire Physiol 295(5)
  • Peer Reviewed
• [Journal Article] Critical role of ABCA1 transporter in sphilgosine 1-phosphate release from astrocytes (2007)
  • Author(s): Sato K, Malchinkhuu E, Horiuchi Y, Mogi C, Tomura H, Tosaka M, Yoshimoto Y, Kuwabara A, Okajima F
  • Journal Title: J Neurochem (in press)
  • Peer Reviewed
• [Journal Article] HDL-like lipoproteins in cerebrospinal fluid affect neural cell activity through lipoprotein-associated sphingosine 1-phoslphate (2007)
  • Author(s): Sato K, Malchinkhuu E, Horiuchi Y, Mogi C, Tomura H, Tosaka M, Yoshimoto Y, Kuwabawa A, Okajima F
  • Journal Title: Biochem Biophys Res Commun 359(3) Pages: 649-654
  • Peer Reviewed
• [Journal Article] Role of lipoprotein-associated lysqphospbolipids in migratory activity of coronary artery smooth muscle cells (2007)
  • Author(s): Damirin A, Tomura H, Komachi M, Liu JP, Mogi C, Tobo M, Wang JQ, Kimura T, Kuwabara A, Yamazaki Y, Ohta H, Im DS, Sato K, Okajima F
  • Journal Title: Am J Physiol Heart Circ Physiol 292(5) Pages: 2513-2522
  • Peer Reviewed
• [Journal Article] Role of scavenger receptor class B type I and sphingosine 1-phosphate receptors in high density lipoprotein-inducedinhibition of adnesion molecule expression in endothelial (2006)
  • Author(s): Kimura T, Tomura H, Mogi C, Kuwabara A, Damirin A, Ishizuka T, Sekiguchi A, Ishiwara M, Im DS Sato K, Murakami M, Okajima F
  • Journal Title: J Biol Chem Dec8 ; 281(49) Pages: 37457-67
  • Peer Reviewed
• [Journal Article] Sphingosine 1-phosphate receptors mediate stimulatory and inhibitory signalings for expression of adhesion molecules in endothelial cells (2006)
  • Author(s): Kimura T, Tomura H, Mogi C, Kuwabara A, Ishiwara M, Shibasawa K, Sato K, Ohwada S, Im DS, Kurose H, Ishizuka T, Murakami M, Okajima F
  • Journal Title: Cell Signal Jun ; 18(6) Pages: 841-50
  • Peer Reviewed
• [Journal Article] Role of scavenger receptor class B type I and sphingosine 1-phosphate receptors in high density lipoprotein-induced inhibition of molecule expression in endothelial cells. (2006)
  • Author(s): Kimura T, Tomura H, Mogi C, Kuwabara A, Damirin A, Ishizuka T, Sekiguchi A, Ishiwara M, Im DS, Sato K, Murakami M, Okajima F
  • Journal Title: J Biol Chem 281(49) Pages: 37457-67