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Understanding the pathophysiology of taste disorders by analysis of taste receptor gene expression in the tongue of patients with taste disorders.

Research Project

Project/Area Number 18590541
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionNihon University

Principal Investigator

IKEDA Minoru  Nihon University, School of Medicine, professor (30130420)

Co-Investigator(Kenkyū-buntansha) KOKUBUN Shinichiro  Nihon University, School of Medicine, Professor (20153520)
TAKAO Kyoichi  Nihon University, School of Medicine, Research Assistant (90187922)
ONODA Keiko  Nihon University, School of Medicine, Assistant Professor (00349995)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,810,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥2,900,000 (Direct Cost: ¥2,900,000)
Keywordstaste-related gene / T2R Family / taste disorder / human / taste disorder due to zinc deficiency / spontaneous abnormal taste
Research Abstract

In this study, we examined the expression of ten genes (TAS2R40, 42, 43, 48, T2R3, 8, 9, 10, 13, and 16) related to the bitter taste receptor in healthy control subjects and in patients with taste disorders. I suggest changing the underlined to" Taste receptor gene expression was examined using RT-PCR. Total RNA was isolated from a sample scraped from the foliate papillae using TRIzol (Invitrogen). The total RNA was reverse transcribed using Superscript III (Invitrogen). The cDNA was subjected to PCR using ExTaq (Takara), and the PCR products were analyzed using a Bioanalyzer 2100 (Agilent).
Patients with taste disorders showed a lower taste receptor gene expression than that of healthy control subjects, with TAS2R40 expressed at significantly lower levels. When considering the causes of taste disorders, cases of taste disorder due to zinc deficiency in particular tended to show lower expression of taste receptor genes. In these cases, TAS2R40 and T2R8 and 10 were expressed at significantly lower levels. TAS2R40 was expressed at significantly lower levels in cases of idiopathic taste disorder, whereas T2R3 showed significantly lower expression in cases with drug-induced taste disorder. Cases with bitter taste disorder showed lower expression of these taste genes compared to that of healthy control subjects. For TAS2R40, 42, T2R3,8 and 9, the lower expression was statistically significant.
These results suggest that that TAS2R40, 42, T2R3, 8, 9and 10 are likely to be related to taste in humans. In addition, these findings can be applied to the diagnosis of taste disorders and in the determination of a prognosis. Clinical application appears most appropriate in cases with taste disorder due to zinc deficiency.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (18 results)

All 2008 2007 2006

All Journal Article (9 results) (of which Peer Reviewed: 2 results) Presentation (8 results) Book (1 results)

  • [Journal Article] 質疑応答。味覚障害と舌痛症2008

    • Author(s)
      池田稔, 野村泰之
    • Journal Title

      日本医事新報 4416号

      Pages: 99-100

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Causative factors of taste disorders and therapeutic effects of zinc agents among aged people.2008

    • Author(s)
      Minoru Ikeda, Akihiro Ikui, Ayako Komiyama, Daisuke Kobayashi, Makoto Tanaka.
    • Journal Title

      The Journal of Laryngology & Otology 122

      Pages: 155-160

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Taste disorders and zinc deficiency.2008

    • Author(s)
      Minoru Ikeda, Rhoji Hirai, Shuntaro Shigihara, Akihiro, Akinori Ikui
    • Journal Title

      International J of Oral-medical Sciences 6

      Pages: 105-111

    • NAID

      10018910800

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Causative factors of taste disorders and therapeutic effects of zinc agents among aged people2008

    • Author(s)
      Minoru Ikeda
    • Journal Title

      The Journal of Laryngology & Otology 122

      Pages: 155-160

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 味覚の障害。脳神経障害の症候と検査Update2007

    • Author(s)
      池田 稔
    • Journal Title

      Clinical Neuroscience 25

      Pages: 1008-1009

    • Related Report
      2007 Annual Research Report
  • [Journal Article] 味覚障害と亜鉛欠乏 特集 : 臨床における亜鉛の有効性の検索2007

    • Author(s)
      池田 稔, 生井明浩
    • Journal Title

      Biomedical Research on Trace Elementsl 18

      Pages: 10-14

    • Related Report
      2006 Annual Research Report
  • [Journal Article] あじ(味覚) 味覚障害の原因・病態2006

    • Author(s)
      池田 稔
    • Journal Title

      脳21 9・2

      Pages: 196-201

    • Related Report
      2006 Annual Research Report
  • [Journal Article] (亜鉛と味覚障害) 味覚障害に対する亜鉛治療とその効果2006

    • Author(s)
      池田 稔
    • Journal Title

      日本味と匂学会誌 13・1

      Pages: 9-16

    • Related Report
      2006 Annual Research Report
  • [Journal Article] (舌疾患の診断と治療) 味覚異常-その機序と治療-2006

    • Author(s)
      池田 稔
    • Journal Title

      ENTONI 70

      Pages: 1-7

    • Related Report
      2006 Annual Research Report
  • [Presentation] The Story of Zinc-日常臨床における亜鉛の役割-。「増加している味覚障害-亜鉛との関連は解明されたか-」2008

    • Author(s)
      池田 稔
    • Organizer
      東京内科医会 第21回医学会
    • Place of Presentation
      東京
    • Year and Date
      2008-03-08
    • Related Report
      2007 Annual Research Report
  • [Presentation] The Story of Zinc-日常臨床における亜鉛の役割- 指名講演「増加している味覚障害-亜鉛との関連は解明されたか-」2008

    • Author(s)
      池田 稔
    • Organizer
      宮城県臨床内科医会学術講演会
    • Place of Presentation
      仙台
    • Year and Date
      2008-02-02
    • Related Report
      2007 Annual Research Report
  • [Presentation] T2R gene family expression in the human tongue : A comparison between normal healthy subjects and patients with taste disorders.2008

    • Author(s)
      M. Ikeda, K. Takao, R Hirai, S. Kokubun, K. Onoda
    • Organizer
      International Symposium on Olfaction and Taste XV
    • Place of Presentation
      San Francisco, USA.
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Taste clinic - A summary : Causative factors and treatment of taste disorder. "Symposium : An approach to clinical taste disorders".2008

    • Author(s)
      M. Ikeda, H. Tomita
    • Organizer
      XVIIth Congress of European Chemoreception Research Organization
    • Place of Presentation
      Portroz, Slovenia.
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] T2R gene family expression in the human tongue : A comparison between normal healthy subjects and patients with taste disorders.2008

    • Author(s)
      M. Ikeda, K. Takao, R. Hirai, S. Kokubun, K. Onoda
    • Organizer
      XVIIth Congress of European Chemoreception Research Organization.
    • Place of Presentation
      Portroz, Slovenia.
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 味覚障害と亜鉛2007

    • Author(s)
      池田 稔
    • Organizer
      静岡県耳鼻咽喉科医会学術講演会
    • Place of Presentation
      静岡
    • Year and Date
      2007-10-13
    • Related Report
      2007 Annual Research Report
  • [Presentation] 「増加している味覚障害-亜鉛との関連は解明されたか」。日常臨床における亜鉛の役割2007

    • Author(s)
      池田 稔
    • Organizer
      広島県内科会 日本臨床内科医会 合同講演会
    • Place of Presentation
      広島
    • Year and Date
      2007-09-08
    • Related Report
      2007 Annual Research Report
  • [Presentation] 味覚障害と亜鉛2007

    • Author(s)
      池田 稔
    • Organizer
      第93回札幌市耳鼻咽喉科医会学術研修会
    • Place of Presentation
      札幌
    • Year and Date
      2007-06-09
    • Related Report
      2007 Annual Research Report
  • [Book] 味覚障害診療の手引き2006

    • Author(s)
      池田 稔
    • Total Pages
      53
    • Publisher
      金原出版株式会社
    • Related Report
      2006 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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