| Project/Area Number |
18590561
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | Saga University |
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Principal Investigator |
ICHIBA Masayoshi Saga University, Faculty of Medicine, Professor (60184628)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Akiko Saga University, Faculty of Medicine, Professor assistant Professor (10330979)
KONDHO Toshihiro Saga University, Analytical Research Center for Experimental Sciences, Research assistant (20186852)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥2,170,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | DNA adduct / alcohol / mice / acetaldehyde |
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| Research Abstract |
The present experiments were designed to determine the effect of drinking ethanol on benzo[a]pyrene (B[a]P)-DNA adduct formation in mice. A positive association between alcohol intake and cancer risk has been found in many epidemiological studies, yet the biological basis for this association is incompletely understood. For this study, mice were treated with ethanol for 8 weeks before exposure to B[a]P at 100 or 20mg/kgBW. 5 days later, DNA adduct of lung and stomach were measured by 32P -postlabeling method. In female alcohol intake mice treated with 100mg/kg, DNA adduct of lung were significantly higher than water intake mice. Especially, higher adduct were found in ALDH2 knock out mice. These studies indicate that ethanol or acetaldehyde can lead to increased formation of B[a]P DNA adduct. Thus, this report provides evidence for a biological mechanism whereby alcohol consumption may increase the risk of cancer.
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