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Evaluation of biological effects of thermally decomposed asbestos

Research Project

Project/Area Number 18590576
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hygiene
Research InstitutionSt.Marianna University School of Medicine

Principal Investigator

TAKATA Ayako  St.Marianna University School of Medicine, Preventive Medicine, Lecturer (30321897)

Co-Investigator(Kenkyū-buntansha) YAMAUCHI Hiroshi  Kitasato University, School of Allied Health Science, Public Health, Professor (90081661)
AMINAKA Masahito  St. Marianna University School of Medicine, Preventive Medicine, Assistant (30231997)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,950,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsasbestos / waste recycling / carcinogenicity / oxidative DNA damage / forsterite / アズベスト
Research Abstract

The conversion of chrysotile (CH) to forsterite (FO) by thermal treatment is considered to be an acceptable technique for detoxifying chrysotile. In this study, the biological effects of CH and an FO that is synthesized by heating CH at 1000℃ were compared with respect oxidative DNA damage and lung injury.
Following the administration of a single 1-mg intratracheal dose of CH or FO, we evaluated histopathological changes in the lung tissue, the lung concentrations of 8-hydroxy-2'-deoxyguanosine (8-OHdG) by HPLC-electrochemical detection (ECD) method, and the expression of 8-OHdG by immunostaining for 540 days. For the control group, we administered a single intratracheal dose of sterile saline.
In the early period following the intratracheal administration of CH, expression of 8-OHdG was observed in the bronchiolar and alveolar epithelial cells as well as in inflammatory cells and granulomas ; at the same time, an increase in the lung concentration of 8-OHdG was observed. As fibrosis progressed, expression of 8-OHdG became more apparent in the airway epithelial and inflammatory cells surrounding the fibrotic lesions ; even at 540 days post-administration, the expression of 8-OHdG was significantly higher than that of the control group. On the other hand, compared with the CH group, the acute lung inflammation observed in the FO group was less apparent and exhibited no progressive fibrosing lesions. The expression of 8-OHdG was transient and weak in the bronchiolar epithelial cells as well as in the inflammatory cells, which was consistent with the low concentration of 8-OHdG in the lungs. This study demonstrates that synthetic FO causes significantly less inflammation and oxidative DNA damage in the lungs than CH.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (15 results)

All 2007 2006

All Journal Article (2 results) Presentation (13 results)

  • [Journal Article] アスベストの無害化に向けて-無害化処理の現状-2007

    • Author(s)
      高田 礼子
    • Journal Title

      安全衛生コンサルタント 27

      Pages: 34-38

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Journal Article] Overview of the detoxification of asbestos : Current state of the decompositon of asbestos-containing waste.2007

    • Author(s)
      Ayako Takata
    • Journal Title

      Safety and Health Consultant 27

      Pages: 34-38

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] クリソタイルの焼成処理から生成した非晶質材料における酸化的DNA損傷評価2007

    • Author(s)
      高田 礼子, 他
    • Organizer
      第55回日本職業・災害医学会学術大会
    • Place of Presentation
      名古屋
    • Year and Date
      2007-11-02
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Evaluation of Oxidative DNA damage induced by the amorphous material decomposed by heating chrysotile.2007

    • Author(s)
      Ayako Takata, et. al.
    • Organizer
      55th Annual Meeting of Japanese Society of Occupational Medicine and Traumatology
    • Place of Presentation
      Nagoya
    • Year and Date
      2007-11-02
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] クリソタイルの焼成処理から生成した非晶質材料における酸化的DNA損傷評価.2007

    • Author(s)
      高田 礼子、他
    • Organizer
      第55回日本職業・災害医学会学術大会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2007-11-02
    • Related Report
      2007 Annual Research Report
  • [Presentation] アスベスト無害化処理材料の生体影響2007

    • Author(s)
      高田 礼子
    • Organizer
      平成19年大気環境学会関東支部講演会
    • Place of Presentation
      東京
    • Year and Date
      2007-06-22
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Biological effects of decomposed asbestos.2007

    • Author(s)
      Ayako Takata
    • Organizer
      2007 Lecture Meeting of Kanto Chapter of Japan Society for Atmospheric Environment
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-06-22
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] アスベスト無害化処理材料の生体影響.2007

    • Author(s)
      高田 礼子
    • Organizer
      平成19年大気環境学会関東支部講演会
    • Place of Presentation
      東京大学一条ホール
    • Year and Date
      2007-06-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] クリソタイル焼成改質材料のラット気管内単回投与による呼吸器影響:酸化的DNA損傷評価2007

    • Author(s)
      高田 礼子, 他
    • Organizer
      第80回日本産業衛生学会
    • Place of Presentation
      大阪
    • Year and Date
      2007-04-26
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Pulmonary toxicity of thermally transformed fiber samples from chrysotile evaluated by single intratracheal injection in rats : Evaluation of oxidative DNA damage.2007

    • Author(s)
      Ayako Takata, et. al.
    • Organizer
      80th Annual Meeting of Japan Society for Occupational Health
    • Place of Presentation
      Osaka
    • Year and Date
      2007-04-26
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] クリソタイル焼成改質材料のラット気管内単回投与による呼吸器影響:酸化的DNA損傷評価.2007

    • Author(s)
      高田 礼子、他
    • Organizer
      第80回日本産業衛生学会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2007-04-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] 石綿無害化処理材料の有害性評価2007

    • Author(s)
      高田 礼子
    • Organizer
      第77回日本衛生学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2007-03-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Hazard assessment of product materials of the innocuous process of asbestos.2007

    • Author(s)
      Ayako Takata
    • Organizer
      77th Annual Meeting of Japan Society for Hygiene
    • Place of Presentation
      Osaka
    • Year and Date
      2007-03-25
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] アスベストの無害化に向けて-非石綿化処理の現状-2006

    • Author(s)
      高田 礼子
    • Organizer
      第54回日本職業・災害医学会学術大会
    • Place of Presentation
      横浜
    • Year and Date
      2006-11-10
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Overview of the decomposition of asbestos : hazard assessment of thermally transformed asbestos.2006

    • Author(s)
      Ayako Takata
    • Organizer
      54th Annual Meeting of Japanese Society of Occupational Medicine and Traumatology
    • Place of Presentation
      Yokohama
    • Year and Date
      2006-11-10
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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