| Budget Amount *help |
¥3,810,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Research Abstract |
1. Identification of molecular structure of hydroperoxy phosphatidylcholine (PC 16:0 / 18:2-OOH), phosphatidylcholine hydroxide (PC 16:0 / 18:2-OH, PC-OH)
Hydroperoxy phosphatidylcholine (PC 16:0 / 18:2-OOH), phosphatidylcholine hydroxide (PC 16:0 / 18:2-OH, PC-OH), epoxyhydroxy-PC, oxo-PC, trihydroxy-PC were identified in human plasma by LC/ESI-MS and LC/ESI-MS-MS. Hydroperoxy lyso-phosphatidylcholine (18:2-OOH) and lyso- phosphatidylcholine oxide (oxo-18:2) were also identified. 18:2, 20:4, 16:0, 18:1, and 18:0 were identified as molecular species of lyso-phosphatidylcholine.
2. Oxidative stress identified by oxisterols as a marker
(1) Alcoholic disorder in rat small intestine and anti-oxidative effect of daizein
Acute ethanol feeding induced accumulation of cholesterol hydroper-oxide and oxysterols in rat small intestine, suggesting that ethanol loading increased oxidative stress. Loading of Daisein in advance canceled such effects.
(2) Fasting stress in rat
Oxidative stress due to fasting was stronger in the first day after fasting than in the second day in rat.
(3) Oxidative stresss due to ischemic-reperfusion in turniquet-release
The enhanced oxidative stresss due to ischemic-reperfusion in turniquet-release mice were observed not only in the gastrocnemius muscles but also in the kidneys and liver.
(4) Liver disorder by D-galactosamine and the effect of anti-oxidant
D-galactosamine loading induced accumulation of cholesterol hydroperoxide and oxysterols in rat liver, brain and gastrocnemius muscles, suggesting that oxidative stress by D-galactosamine depend on organs or tissues.
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