Elucidation of molecular structure of lipid peroxides as oxidative suns markers and and application of those to legal medicine
| Project/Area Number |
18590633
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Kobe University |
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Principal Investigator |
UENO Yasuhiro Kobe University, Graduate School of Medicine, Professor (30184956)
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| Co-Investigator(Kenkyū-buntansha) |
ASANO Migiwa Kobe University, Graduate School of Medicine, Research associate (90283879)
NUSHIDA Hideyuki Kobe University, Graduate School of Medicine, Research associate (90335448)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,810,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | lipid peroxidation / hydroperoxy phos hatidvlcholine / alcohol / fasting / oxidative stress / oxysterol / anti-oxidant / turniouet-release |
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| Research Abstract |
1. Identification of molecular structure of hydroperoxy phosphatidylcholine (PC 16:0 / 18:2-OOH), phosphatidylcholine hydroxide (PC 16:0 / 18:2-OH, PC-OH)
Hydroperoxy phosphatidylcholine (PC 16:0 / 18:2-OOH), phosphatidylcholine hydroxide (PC 16:0 / 18:2-OH, PC-OH), epoxyhydroxy-PC, oxo-PC, trihydroxy-PC were identified in human plasma by LC/ESI-MS and LC/ESI-MS-MS. Hydroperoxy lyso-phosphatidylcholine (18:2-OOH) and lyso- phosphatidylcholine oxide (oxo-18:2) were also identified. 18:2, 20:4, 16:0, 18:1, and 18:0 were identified as molecular species of lyso-phosphatidylcholine.
2. Oxidative stress identified by oxisterols as a marker
(1) Alcoholic disorder in rat small intestine and anti-oxidative effect of daizein
Acute ethanol feeding induced accumulation of cholesterol hydroper-oxide and oxysterols in rat small intestine, suggesting that ethanol loading increased oxidative stress. Loading of Daisein in advance canceled such effects.
(2) Fasting stress in rat
Oxidative stress due to fasting was stronger in the first day after fasting than in the second day in rat.
(3) Oxidative stresss due to ischemic-reperfusion in turniquet-release
The enhanced oxidative stresss due to ischemic-reperfusion in turniquet-release mice were observed not only in the gastrocnemius muscles but also in the kidneys and liver.
(4) Liver disorder by D-galactosamine and the effect of anti-oxidant
D-galactosamine loading induced accumulation of cholesterol hydroperoxide and oxysterols in rat liver, brain and gastrocnemius muscles, suggesting that oxidative stress by D-galactosamine depend on organs or tissues.
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Report
(3 results)
Research Products
(34 results)
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[Journal Article] Skeletal muscle and liver oxysterols during fasting and alcohol exposure2006
Author(s)
Adachi, J, Kudo, R, Asano, M, Ueno, Y, Hunter, R, Rajendram, R, Martin, C, Preddy, VR
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Journal Title
Metabolism 55
Pages: 119-127
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Enhanced lipid peroxidation in tourniquet-release mice2006
Author(s)
Adachi, J, Kurisaki, E, Kudo, R, Nakagawa, K, Hatake, K, Hiraiwa, K, Ueno, Y
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Journal Title
Clin Chitn Acta 371
Pages: 79-84
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Adipocele formed case in dry concealment2006
Author(s)
Nushida, H, Asano, M, Nakagawa, K, Adachi, J, Nagasaki, Y, Ueno, Y
Organizer
The 53th Kinki District Conference of the Japanese Society of Legal Medicine
Place of Presentation
Osaka
Year and Date
2006-11-11
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Analysis of lipids in adipocere by GC-MS2006
Author(s)
Nushida, H, Adachi, J, Yoshioka, N, Asano, M, Ueno, Y
Organizer
The 31st annual meeting of the Japanese Society for Biomedical Mass Spectromerty
Place of Presentation
Nagoya
Year and Date
2006-09-28
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Alcohol ingestion and oxidative stress in rat small interstine2006
Author(s)
Nakagawa, K, Adachi, J, Saeki, Y, Nushida, H, Asano, M, Ueno, Y
Organizer
The 90th Congress of the Japanese Society of Legal Medicine
Place of Presentation
Fukuoka
Year and Date
2006-04-28
Description
「研究成果報告書概要(欧文)」より
Related Report
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