Cholinergic function in apolipoproteinE (apoE-/-) and ALDH2 (-/-) mice after alcohol consumption, and the relation of alcohol in producing dementia in these animal models.

• Project/Area Number: 18590637
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Kagawa University
• Principal Investigator: JAMAL MOSTOFA Kagawa University, 医学部, 助教 (50418802)
• Co-Investigator(Kenkyū-buntansha): AMENO Kiyoshi 香川大学, 医学部, 准教授 (50019626)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,100,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2007: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2006: ¥2,800,000 (Direct Cost: ¥2,800,000)
• Keywords: アルコール / アセトアルデヒド / コリン作動性神経系 / 前頭皮質 / ALDH2ノックアウトマウス / ApoEノックアウトマウス / 記憶の固定 / 8アームラジアル迷路 / Alcohol / Acetaldehyde / Cholinergic function / Frontal Cortex / Aldh2(- / -)mice / ApoE(- / Memory consolidation / Eight-arm radial maze / frontal cortex / hippocampus / RT-PCR / Western blot / acetylcholinesterase / choline acetyltransferase / cholinergic function / エタノール / 前頭葉

Research Abstract

アルコール(EtOH)投与後のALDH2 KOマウスは血液および脳内にEtOHとともに高濃度のアセトアルデヒドを検出した。EtOH投与によりALDH2 KOマウスの前頭皮質のコリンアセチルトランスフェラーゼ(ChAT)とアセチルコリンエステラーゼ(AChE)発現の抑制を、野生型マウスでは多量のEtOH投与にのみChAT発現の抑制を認めた。8アームラジアル迷路実験では、ALDH2 KOマウスはEtOH投与量依存的に、ApoE KO、野生型マウスでは多量投与でのみ有意なエラー増加を認めた。

Research Products

• [Journal Article] Shiakh Badiuzzaman and Iwao Ijiri. Acetaldehyde-induce changes in cholinergic function in the frontal cortex of ALDH2 transgenic mice (3^<rd>) (2008)
  • Author(s): Mostofa Jamal, Kiyoshi Ameno, Uekita Ikuo, Weihuan Wang, Mitsuru Kumihashi
  • Journal Title: Jpn J Legal Medicine(Reviewed) 62(suppl)
• [Journal Article] Cholinergic changes following exposure to nicotine, ethanol, or both combined in the frontal cortex and hippocampus of rat (2008)
  • Author(s): Mostofa Jamal, Ikuo Uekita, Mitsuru Kumihashi, Iwao Ijiri, and Kiyoshi Ameno
  • Journal Title: Jpn J Alcohol & Drug Dependence(Reviewed) 43 Pages: 384-385
  • NAID: 10021950593
• [Journal Article] Acetaldehyde-induce changes in cholinergic function in the frontal cortex of ALDH2 transgenic mice (3^<rd>) (2008)
  • Author(s): Mostofa Jamal, Kiyoshi Ameno, Uekita Ikuo, Weihuan Wang, Mitsuru Kumihashi, Shiakh Badiuzzaman, Iwao Ijiri
  • Journal Title: Jpn J Legal Med 62 Pages: 57-57
  • Peer Reviewed
• [Journal Article] Chollnergic changes following exposure to nicotine, ethanol, or both combined in the frontal cortex and hippocampus of rat (2008)
  • Author(s): Mostofa Jamal, Ikuo Dekita, Mitsuru Kumihashi, Iwao Ijiri, Kiyoshi Ameno
  • Journal Title: J Alcohol & Drug Dependence 43 Pages: 384-385
  • Peer Reviewed
• [Journal Article] Catalase mediates acetaldehyde formation in the striatum of free-moving rats (2007)
  • Author(s): Mostofa Jamal, Kiyoshi Ameno, Ikuo Uekita, Mitsuru Kumihashi, Weihuan Wang and Iwao Ijiri
  • Journal Title: Neurotoxicology(Reviewed) 28 Pages: 1245-1248
• [Journal Article] Changes in cholinergic function in the frontal cortex and hippocampus of rat exposed to ethanol and acetaldehyde (2007)
  • Author(s): Mostofa Jamal, Kiyoshi Ameno, Setsuko Ameno, Jun Morishita, Weihuan Wang, Mitsuru Kumihashi, Uekita Ikuo, Takanori Miki and Iwao Ijiri
  • Journal Title: Neuroscience(Reviewed) 144 Pages: 232-23
• [Journal Article] Effect of direct Infusion of Acetaldehyde on Dopamine and Dopamine-derived Salsolinol in the striatum of free-moving rats using reverse microdialysis (2007)
  • Author(s): Wang W, Ameno K, Jamal M, Kumihashi M, Uekita I, Ameno S, ljiri I.
  • Journal Title: Arch Toxicol 81 Pages: 121-126
  • Peer Reviewed
• [Journal Article] Simultaneous determination of Metamphetamine and Amphetamine in urine using a high performance liquid chromatography co lumn switching method (2007)
  • Author(s): Kumihashi M, Ameno K, Shibayama T, Suga K, Miyauchi H, Jamal M, Wang W, Uekita I, Ijiri I
  • Journal Title: J Chromatogr B 845 Pages: 180-183
  • Peer Reviewed
• [Journal Article] Changes in cholinergic funct ion in the frontal cortex and hippocampus of rat exposed to ethanol and acetaldehyde (2007)
  • Author(s): Jamal M, Ameno K, Ameno S, Morishita J, Wang W, Kumihashi M, Uekito I, Miki T, Ijiri I
  • Journal Title: Neuroscience 144 Pages: 232-238
  • Peer Reviewed
• [Journal Article] Hyperpyrexia induced by a multiple antidepressants overdose (2007)
  • Author(s): Kinoshito H, Kubota A, Nishiguchi M, Ouchi H, Minami T, Matsui K, Yamamura T, Motomura H, Kuboyama K, Marukawa S, Ameno K, Hishida S.
  • Journal Title: Human & Experimental Toxicology 26 Pages: 609-611
  • Peer Reviewed
• [Journal Article] Catalase mediates acetaldehyde formation in the striatum of free-moving rats (2007)
  • Author(s): Jamal M, Ameno K, Uekita I, Kumihashi M, Wang W, ljiri I.
  • Journal Title: NeuroToxicology 28 Pages: 1245-1248
  • Peer Reviewed
• [Journal Article] Ethanol and aceta ldehyde: in vivo quantitation and effects on cholinergic function in ratbrain (2007)
  • Author(s): Jamal M, Ameno K, Uekita I, Kumihashi M, Wang W, ljiri I.
  • Journal Title: Novartis Foundation Symp 285 Pages: 137-141
  • Peer Reviewed
• [Journal Article] 瀬戸内海で発見された海中死体の手背部の状態による死後経過時間の推定 (2007)
  • Author(s): 三澤 宏規、上北 郁男、王 威環、ジャマール モストファ、飴野 清、井尻 巖
  • Journal Title: 法医学の実際と研究 50 Pages: 205-209
  • Peer Reviewed
• [Journal Article] Changes in cholinergic function in the frontal cortex and hippocampus of rat exposed to ethanol and acetaldehyde. (2007)
  • Author(s): Mostofa Jamal, et al.
  • Journal Title: Neuroscience 144 Pages: 232-238
• [Presentation] Cholinergic changes following exposure to nicotine, ethanol, or both combined in the frontal cortex and hippocampus of rat. (2008)
  • Author(s): Mostofa Jamal, Ikuo Uekita, Mitsuru Kumihashi, Iwao Ijiri, and Kiyoshi Ameno.
  • Organizer: 11^<th> Conference of the Japanese Forum on Nicotine and Drug Dependence Studies
  • Place of Presentation: Yokohama
• [Presentation] Acetaldehyde-induce changes in cholinergic function in the frontal cortex of ALDH2 transgenic mice (3^<rd>). (2008)
  • Author(s): Mostofa Jamal, Kiyoshi Ameno, Uekita Ikuo, Weihuan Wang, Mitsuru Kumihashi, Shiakh Badiuzzaman and Iwao Ijiri.
  • Organizer: 5th International Symposium on Advances in Legal Medicine (ISALM)
  • Place of Presentation: Osaka
• [Presentation] Nagasaki : Role of catalase in mediating acetaldehyde production in the striatum of free-moving rats (2008)
  • Author(s): Mostofa Jamal, Kiyoshi Ameno, Ikuo Uekita, Mitsuru Kumihashi, Weihuan Wang, and Iwao Ijiri.
  • Organizer: 92th Congress of Japanese Society of Legal Medicine(Poster)(Reviewed)
  • Place of Presentation: Nagasaki
• [Presentation] Role of catalase in mediating acetaldehyde production in the striatum of free-moving rats (2008)
  • Author(s): Mostofa Jamal, Kiyoshi Ameno, Ikuo Uekita, Mitsuru Kumihashi, Weihuan Wang, Iwao Ijiri
  • Organizer: 92th Congress of Japanese Society of Legal Medicine
  • Place of Presentation: Nagasaki
• [Presentation] Acetaldehyde-induce changes in cholinergic function in the frontal cortex of ALDH2 transgenic mice (2008)
  • Author(s): Mostofa Jamal, KiyoshiAmeno, Uekita Ikuo, Weihuan Wang, Mitsuru Kumihashi, Shiakh Badiuzzaman, Iwao Ijiri
  • Organizer: 5th International Symposium on Advances in Legal Medicine (ISALM)
  • Place of Presentation: Osaka
• [Presentation] Cholinergic changes following exposure to nicotine, ethanol, or both combined in the frontal cortex and hippocampus of rat (2008)
  • Author(s): Mostofa Jamal, Ikuo Uekita, Mitsuru Kumihashi, Iwao Ijiri, Kiyoshi Ameno.
  • Organizer: 11^<th> Conference of the Japanese Forum on Nicotine and Drug Dependence Studies
  • Place of Presentation: Yokohama
• [Presentation] Acetaldehyde-induce changes in cholinergic function in the frontal cortex of ALDH2 trangenic mice (2007)
  • Author(s): Jamal M, Ameno K, Wang W, Uekita I, Kumihashi M, lsse T, Kawamoto T, Kitagawa K, Nakayama K, ljiri I.
  • Organizer: 第9回Aldh2ノックアウトマウス学会
  • Place of Presentation: 福岡
  • Year and Date: 2007-10-27
• [Presentation] Acetaldehyde-induce changes in cholinergic function in the frontal cortex of ALDH2 Transgenic Mice. (2007)
  • Author(s): Mostofa Jamal, Kiyoshi Ameno, Weihuan Wang, Ikuo Uekita, Mitsuru Kumihashi, Masashi Higuchi, and Iwao Ijiri.
  • Organizer: 9^<th> Aldh2 Knockout Mice Conference(Oral) (Reviewed)
  • Place of Presentation: Fukuoka
• [Presentation] Acetaldehyde-induce changes in cholinergic function in the frontal cortex of ALDH2 transgenic mice (2007)
  • Author(s): Mostofa Jamal, Kiyoshi Ameno, Weihuan Wang, Ikuo Uekita, Mitsuru Kumihashi and Iwao Ijiri
  • Organizer: 18^<th> Annual Spring Brain Conference (SBC)(Poster)
  • Place of Presentation: Sedona, Arizona, USA
• [Book] Bridging the Trans-Disciplinary Divide Novartis Foundation 285(John Wiley & Sons Ltd, UK) (2007)
  • Author(s): Mostofa Jamal, Kiyoshi Ameno, Mitsuru Kumihashi, Weihuan Wang, Uekita Ikuo and Iwao Ijiri
  • Publisher: Ethanol and acetaldehyde : In vivo quantitation and effects on cholinergic function in rat brain. In : Acetaldehyde-Related PathologyPathology
• [Book] Acetaldehyde-Related Pathology : Bridging the Trans-Disciplinary Divide,Ed,Derek J. Chadwick and Jamie Goode (2007)
  • Author(s): Mostofa Jamal, et al.
  • Total Pages: 5
  • Publisher: Novartis Foundation