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Dermal wound aging by molecular biological detections of growth factors

Research Project

Project/Area Number 18590642
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionIwate Medical University

Principal Investigator

AOKI Yasuhiro  Iwate Medical University, School of Medicine, Professor (90202481)

Co-Investigator(Kenkyū-buntansha) TAKAMIYA Masataka  Iwate Medical University, School of Medicine, Lecturer (30364334)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥2,700,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsskin / brain / bone / cytokine / growth factor / real-time quantitative PCR / in situ hybridization / beads suspension array / 損傷 / beads suspension array / quantitave PCR / エイジング / 創傷治癒 / 定量的PCR
Research Abstract

Dermal wound age provides forensic pathologists with valuable information for the reconstruction of crime scenes and determination of the cause of death. In this study, cytokine expressions in murine skins were analyzed using immunohistochemical methods, real-time quantitative PCR, and in situ hybridization techniques. Wound age estimation for human dermal wounds was also performed based on quantification of 17 cytokines. The potential parameters would be IL 5, IL 10, IL 12 p 70, IL 13, IL 17, GM-CSF, IFN γ, TNF α in the early phase of dermal wound healing, IL 6, MCP 1 in the middle phase, and IL 1 β, IL 2, IL 4, IL 8, G-CSF, and MIP 1β in the middle and late phases. In addition, an outsourced examination of IL 1 β, IL 4, IL 6, IL 8, IL 10, G-CSF, GM-CSF, IFN γ, MCP1, TNFα, which could be available to any forensic institute, was performed for confirmative purposes. Many factors have been proposed as markers for dermal wound age estimation, but the set of cytokines selected for the outsourced examination in the present study wound be useful in daily forensic practice.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (29 results)

All 2008 2007 2006 Other

All Journal Article (21 results) (of which Peer Reviewed: 10 results) Presentation (8 results)

  • [Journal Article] Simultaneous detection of eight cytokines in human dermal wounds with a multiplex bead-based immunoassay for wound age estimation2008

    • Author(s)
      Masataka Takamiya, Saschiko Fujita, Kiyoshi Saigusa, Yasuhiro Aoki.
    • Journal Title

      International Journal of Legal Medicine 122

      Pages: 143-148

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A study on mRNA expressions of interleukin 10 during fracture healing for wound age determination2008

    • Author(s)
      Masataka Takamiya, Sachiko Fujita, Kiyoshi Saigusa, Yasuhiro Aoki.
    • Journal Title

      Legal Medicine 10

      Pages: 131-137

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Simultaneous detection of eight cytokines in human dermal wounds with a multiplex bead-based immunoassay for wound age estimation.2008

    • Author(s)
      Masataka Takamiya, Saschiko Fujita, Kiyoshi - Saigusa, Yasuhiro Aoki
    • Journal Title

      International Journal of Legal Medicine 122

      Pages: 143-148

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A study on mRNA expressions of interleukin 10 during fracture healing for wound age determination.2008

    • Author(s)
      Masataka Takamiya, Sachiko Fujita, Kiyoshi Saigusa, Yasuhiro Aoki
    • Journal Title

      Legal Medicine 10

      Pages: 131-137

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Simultaneous detection of eight cytokines in human dermal wounds with a multiplex bead-based immunoassay for wound age estimation2008

    • Author(s)
      Masataka Takamiya, Sachiko Fujita, Kiyoshi Saigusa, Yasuhiro Aoki.
    • Journal Title

      International Journal of Legal Medicine 122

      Pages: 143-148

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous detections of 27 cyrtokines during cerebral wound healing by multiplexed bead-based immunoassay for wound age estimation2007

    • Author(s)
      Masataka Takamiya, Sachiko Fujita, Kiyoshi Saigusa, Yasuhiro Aoki.
    • Journal Title

      Journal of Neurotrauma 24

      Pages: 1833-1844

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Simultaneous detections of 27 cytokines during cerebral wound healing by multiplexed bead-based immunoassay for wound age estimation.2007

    • Author(s)
      Masataka Takamiya, Sachiko Fujita, Kiyoshi Saigusa, Yasuhiro Aoki
    • Journal Title

      Journal of Neurotrauma. 24

      Pages: 1833-1844

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A study on mRNA expressions of fibronectin in dermal and cerebral wound healing for wound age estimation2006

    • Author(s)
      Masataka Takamiya, Reiko Kumagai, Nori Nakayashiki, Yasuhiro Aoki.
    • Journal Title

      Legal Medicine 8

      Pages: 214-219

    • NAID

      10024171554

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A case of carbamazepine overdose with focal myocarditis2006

    • Author(s)
      Masataka Takamiya, Yasuhiro Aoki, Hisae Niitsu, Kiyoshi Saigusa.
    • Journal Title

      Legal Medicine 8

      Pages: 243-247

    • NAID

      10024171666

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A study on mRNA expressions of fibronectin in dermal and cerebral wound healing for wound age estimation.2006

    • Author(s)
      Masataka Takamiya, Reiko Kumagai, Nori Nakayashiki, Yasuhiro Aoki
    • Journal Title

      Legal Medicine 8

      Pages: 214-219

    • NAID

      10024171554

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A case of carbamazepine overdose with focal myocarditis.2006

    • Author(s)
      Masataka Takamiya, Yasuhiro Aoki, Hisae Niitsu, Kiyoshi Saigusa
    • Journal Title

      Legal Medicine 8

      Pages: 243-247

    • NAID

      10024171666

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A study on mRNA expressions of fibronectin in dermal and cerebral wound healing for wound age estimation.2006

    • Author(s)
      Takamiya M, Kumagai R, Nakayashiki N, Aoki Y
    • Journal Title

      Legal Medicine 8(4)

      Pages: 214-219

    • NAID

      10024171554

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A case of carbamazepine overdose with focal myocarditis.2006

    • Author(s)
      Takamiya M, Aoki Y, Niitsu H, Saiguwa K
    • Journal Title

      Legal Medicine 8(4)

      Pages: 243-247

    • NAID

      10024171666

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A case of Takayasu's arteritis complicated by right atrium perforation and injuries of the right common iliac artery and vein caused by cannulation for percutaneous cardiopulmonary support

    • Author(s)
      Masataka Takamiya, Sashiko Fujita, Hisae Niitsu, Yasuhiro Aoki, Hiroyuki Kanno, Takashi Sawai.
    • Journal Title

      American Journal of Forensic medicine and Pathology (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] An autopsy case related to a terrorist attack using a ball-bearing bomb

    • Author(s)
      Masataka Takamiya. Hitoshi Biwasaka, Hisae Niitsu, Kiyoshi Saigusa, Yasuhiro Aoki.
    • Journal Title

      Legal Medicine (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] An autopsy case of aortitis resulting in sudden death due to a rupture of aneurysm of the aortic sinus

    • Author(s)
      Yasuhiro Aok, Masataka Takamiya, Hisae Niitsu, Sachiko Fujita, Kiyoshi Saigusa.
    • Journal Title

      Legal Medicine (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Acute epiglottitis in adults : Report of an autopsy case and review of recent literature

    • Author(s)
      Yasuhiro Aoki, Masataka Takamiya, Hisae Niitsu, Sachiko Fujita, Kirito Shimamoto.
    • Journal Title

      Research and Practice in Forensic Medicine (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A case of Takayasu's arteritis complicated by right atrium perforation and injuries of the right common iliac artery and vein caused by cannulation for percutaneous cardiopulmonary support.

    • Author(s)
      Masataka Takamiya, Sashiko Fujita, Hisae Niitsu, Yasuhiro Aoki, Hiroyuki Kanno, Takashi Sawai
    • Journal Title

      American Journal of Forensic medicine and Pathology (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] An autopsy case related to a terrorist attack using a ball-bearing bomb.

    • Author(s)
      Masataka Takamiya. Hitoshi Biwasaka, Hisae Niitsu, Kiyoshi Saigusa, Yasuhiro Aoki
    • Journal Title

      Legal Medicine (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] An autopsy case of aortitis resulting in sudden death due to a rupture of aneurysm of the aortic sinus.

    • Author(s)
      Yasuhiro Aoki, Masataka Takamiya, Hisae Niitsu, Sachiko Fujita, Kiyoshi Saigusa
    • Journal Title

      Legal Medicine (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Acute epiglottitis in adults : Report of an autopsy case and review of recent literature.

    • Author(s)
      Yasuhiro Aoki, Masataka Takamiya, Hisae Niitsu, Sachiko Fujita, Kirito Shimamoto
    • Journal Title

      Research and Practice in Forensic Medicine (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 皮膚損傷受傷後経過時間推定を目的としたサイトカイン発現の多因子分析2008

    • Author(s)
      高宮正隆, 琵琶坂仁, 藤田さちこ, 三枝聖, 青木康博.
    • Organizer
      第92次日本法医学会総会
    • Place of Presentation
      長崎市
    • Year and Date
      2008-04-24
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Wound age estimation by simultaneous detection of 17 cytokines in human dermal wounds with a multiplex bead-based immunoassay : an estimative method using outsourced examinations.2008

    • Author(s)
      Masataka Takamiya, Hitoshi Biwasaka, Sachiko Fujita, Kiyoshi Saigusa, Yasuhiro Aoki
    • Organizer
      92nd congress of the Japanese Societyof Legal Medicine
    • Place of Presentation
      Nagasaki city
    • Year and Date
      2008-04-24
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ベアリング爆弾による1死亡例2007

    • Author(s)
      高宮正隆, 藤田さちこ, 新津ひさえ, 青木康博.
    • Organizer
      第91次日本法医学会総会
    • Place of Presentation
      秋田市
    • Year and Date
      2007-05-18
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 脳損傷受傷後経過時間推定を目的としたサイトカイン発現の多因子分析2007

    • Author(s)
      高宮正隆, 藤田さちこ, 三枝聖, 青木康博.
    • Organizer
      第91次日本法医学会総会
    • Place of Presentation
      秋田市
    • Year and Date
      2007-05-18
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] An autopsy case related to a terrorist attack using a ball-bearing bomb.2007

    • Author(s)
      Masataka Takamiya, Sachiko Fujita, Hisae Niitsu, Yasuhiro Aoki
    • Organizer
      91st congress of the Japanese Societyof Legal Medicine
    • Place of Presentation
      Akita city
    • Year and Date
      2007-05-18
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Simultaneous detections of 27 cytokines during cerebral wound healing by multiplexed bead-based immunoassay for wound age estimation.2007

    • Author(s)
      Masataka Takamiya, Sachiko Fujita, Kiyoshi Saigusa, Yasuhiro Aoki
    • Organizer
      91st congress of the Japanese Societyof Legal Medicine
    • Place of Presentation
      Akita city
    • Year and Date
      2007-05-18
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 骨折治癒過程におけるtPAおよびuPA遺伝子の発現動態2006

    • Author(s)
      高宮正隆, 熊谷礼子, 中屋敷徳, 青木康博.
    • Organizer
      第90次日本法医学会総会
    • Place of Presentation
      福岡市
    • Year and Date
      2006-04-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] A study on mRNA expressions of tPA and uPA during fracture healing for wound age determination.2006

    • Author(s)
      Masataka Takamiya, Reiko Kumagai, Nori Nakayashiki, Yasuhiro Aoki
    • Organizer
      90th congress of the Japanese Society of Legal Medicine
    • Place of Presentation
      Fukuoka city
    • Year and Date
      2006-04-27
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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