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Development of assay methods for lipid peroxidation products as oxidative stress markers, and their applications to clarify the pathophysiological implication in infectious disorders

Research Project

Project/Area Number 18590644
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionJuntendo University

Principal Investigator

KIMURA Hiroko  Juntendo University, 医学部, 准教授 (00053299)

Co-Investigator(Kenkyū-buntansha) UCHIDA Koji  名古屋大学大学院, 生命農学研究科, 准教授 (40203533)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,910,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥510,000)
Fiscal Year 2008: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywords4-ヒドロキシ2-ノネナール / ニトロチロシン / ニトロトリプトファン / 睡眠時無呼吸症候群 / 酸化ストレス / 時間分解蛍光イムノアッセイ / 4-ヒドロキシ2ノネナール / LPS / 自然高血圧発症ラット
Research Abstract

酸化ストレスマーカーである過酸化脂質, 4-Hydoroxy nonenal(HNE)やニトロ化物の3-nitrotyrosine(NT), 6-nitrotryptophanの時間分解蛍光イムノアッセイを開発し, lipopolysaccharide(LPS)投与ラット(感染症モデルラット)の心臓・大動脈・腎臓・肝臓・腸管などにおけるHNEや他の酸化ストレスマーカーの生成量の測定, 酸化ストレス発生源の同定を行い, その感染症病態における関与の解明を行った. また高血圧を発症した自然発症高血圧ラット(SHR)に持続的な運動を行わせて運動がスーパーオキシドジスムターゼ(Mn-SOD)合成を増加させて酸化ストレスが減少する仕組みを解明した.

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (26 results)

All 2009 2008 2007 2006

All Journal Article (19 results) (of which Peer Reviewed: 17 results) Presentation (7 results)

  • [Journal Article] Upregulation of Heme Oxygenase-1 in an Animal Model of Takotsubo Cardiomyopathy2009

    • Author(s)
      Ueyama T, Kawabe T, Hano T, Tsuruo Y, Ueda K, Ichinose M, Kimura H, Yoshida K.
    • Journal Title

      Circ J 73

      Pages: 1141-1146

    • NAID

      10025931795

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Adaptive HNE-Nrf2-HO1 pathway against oxidative stress is associated with acute gastric mucosal lesions2009

    • Author(s)
      Ueda K, Ueyama T, Yoshida KI, Kimura H, 他6名
    • Journal Title

      Am J Physiol 295

      Pages: 460-469

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel sensitive immunoassay method based on the Invader technique2008

    • Author(s)
      Xie MJ, Fukui K, Horie M, Kimura H, 他3名
    • Journal Title

      Anal Biochem 374

      Pages: 278-284

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mental stress induces sustained elevation of blood pressure and lipid peroxidation in postmenopausal women2008

    • Author(s)
      Morimoto K, Morikawa M, Kimura H, 他5名
    • Journal Title

      Life Sci 82

      Pages: 99-107

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quantitative evaluation of time-resolved fluorescence microscopy using a new europium label : Application to immunofluorescence imaging of nitrotyrosine in kidneys2008

    • Author(s)
      Kimura H, Mukaida M, Watanabe M, 他5名
    • Journal Title

      Anal Biochem 372

      Pages: 119-121

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 高血圧発症後の持久的運動トレーニングが自然発症高血圧ラット心臓のニトロ化ストレスに及ぼす影響2008

    • Author(s)
      古川覚, 木村博子, 向田政博, 他3名
    • Journal Title

      順天堂医学 54

      Pages: 308-317

    • NAID

      110007125435

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A lipid-derived endogenous inducer of COX-2 : a bridge between inflammation and oxidative stress2008

    • Author(s)
      Uchida K.
    • Journal Title

      Mol Cells 25

      Pages: 347-51

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quantitative evaluation of time-resolved fluorescence microscopyusing a new europium label : Application to immunofluorescence imaging of nitrotyrosine in kidneys.2008

    • Author(s)
      Hiroko Kimura
    • Journal Title

      Analytical Biochemistry 372

      Pages: 119-121

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mental stress induces sustained elevation of blood pressure and lipid peroxidation in postmenopausal women.2008

    • Author(s)
      Keiko Morimoto
    • Journal Title

      Life Sciences 82

      Pages: 99-107

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adaptive HNE-Nrf2-H01 pathway against oxidative stress is associated with acute gastric mucosal lesions.2008

    • Author(s)
      Kazuki Ueda
    • Journal Title

      Am J Physiol Gastrointest Liver Physiol 295

      Pages: 460-469

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mental stress induces sustained elevation of blood pressure and lipid peroxidation in postmenopausal women2008

    • Author(s)
      Keiko Morimoto
    • Journal Title

      Life Sciences 82

      Pages: 99-107

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantitative evaluation of time-resolved fluorescence microscopy using a new europium label:Application to immunofluorescence imaging of nitrotyrosine in kidneys2008

    • Author(s)
      Hiroko Kimura
    • Journal Title

      Analytical Biochemistry 372

      Pages: 119-121

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel sensitive immunoassay method based on the Invader technique2008

    • Author(s)
      Min-Jue Xie
    • Journal Title

      Analytical Biochemistry 374

      Pages: 278-284

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a micropulverized extraction method for rapid toxicological analysis of methamphetamine in hair2007

    • Author(s)
      Miyaguchi H, Kakuta M, Iwata YT, Kimura H, 他3名
    • Journal Title

      J Chromatogr A 1163

      Pages: 43-48

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lipid peroxidation and redox-sensitive signaling pathways2007

    • Author(s)
      Uchida K.
    • Journal Title

      Curr Atheroscler Rep 9

      Pages: 216-21

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ischemic preconditioning or p38 MAP kinase inhibition attenuates myocardial TNF α production and mitochondria damage in brief myocardial ischemia2006

    • Author(s)
      Kimura H, Shintani-Ishida K, Nakajima M, 他3名
    • Journal Title

      Life Sci 78

      Pages: 1901-1910

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 4-Hydroxynonenal modifies IgA in rat intestine after lipopolysaccharide injection2006

    • Author(s)
      Kimura H, Mukaida M, Kuwabara K, Uchida K, 他4名
    • Journal Title

      Free Radic Biol Med(内田浩二) 41

      Pages: 973-978

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ischemic preconditioning or p38 MAP kinase inhibition attenuates myocardial TNF α production and mitochondria damage in brief myocardial ischemia.2006

    • Author(s)
      Kimura H, Shintani-Ishida K, Nakajima M, Liu S, Matsumoto K, Yoshida K
    • Journal Title

      Life Sci 78

      Pages: 1901-1910

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 4-Hydroxynonenal modifies IgA in rat intestine after lipopolysaccharide injection.2006

    • Author(s)
      Kimura H, Mukaida M, Kawabara K, Ito T, Hashino K, Uchida K, Matsumoto K, Yoshida K.
    • Journal Title

      Free Radic Biol Med 41

      Pages: 973-978

    • Related Report
      2006 Annual Research Report
  • [Presentation] Quantification of 4-hydroxynonenal (HNE) induced by lipopolysaccharide in rat liver and identification of the HNE-modified protein, a fragment of GRP782008

    • Author(s)
      Kimura H, Mukaida M, Mizukami Y, Matsumoto K, Yoshida K, Kon N.
    • Organizer
      Biomarkers of Oxidative Stress in Health and Diseases. HSSRC/AIST/NIH Joint International Symposium
    • Place of Presentation
      Osaka
    • Related Report
      2008 Final Research Report
  • [Presentation] Quantification of 4-hydroxynonenal(HNE) induced by lipopolysaccharide in rat liver and identification of the HNE-modified protein, a fragment of GRP782008

    • Author(s)
      Kimura H, Mukaida M, Mizukami Y, Matsumoto K, Yoshida K, Kon N.
    • Organizer
      7th International Symposium "Advances in LEGAL MEDICINE" Jpn J Legal Med
    • Place of Presentation
      Osaka
    • Related Report
      2008 Final Research Report
  • [Presentation] Effect of endurance exercise training after emergence of hypertension on nitrative stress for heart of spontaneously hypertensive rat2008

    • Author(s)
      Furukawa S, Kimura H, Mukaida M, Kohno H, Ikeda K, Naito H, Kakigi R, Yamakura F.
    • Organizer
      7th World Congress on Aging and Physical Activity
    • Place of Presentation
      Tsukuba
    • Related Report
      2008 Final Research Report
  • [Presentation] Quantification of 4-hydroxynonenal(HNE)induced by lipopolysaccharide in rat liver and identification of the HNE-modified protein, a fragment of GRP78.2008

    • Author(s)
      Hiroko Kimura
    • Organizer
      Biomarkers of Oxidative Stress in Health and Diseases. HSSRC/AIST-NIEHS/NIH JointInternational Symposium
    • Place of Presentation
      Osaka, Japan
    • Related Report
      2008 Annual Research Report
  • [Presentation] Quantification of 4-hydroxynonenal(HNE)induced by lipopolysaccharide in rat liver and identification of HNE-modified protein,a fragment of GRP782008

    • Author(s)
      Hiroko Kimura
    • Organizer
      Biomarkers of Oxidative Stress in Health and Diseases
    • Place of Presentation
      Osaka,Japan
    • Related Report
      2007 Annual Research Report
  • [Presentation] Function of sex hormones : their roles in the physiological responses to environmental stresses2007

    • Author(s)
      Morimoto K, Uji M, Morikawa M, Hara Y, Kohno T, Takamata A, Kimura H, Ueyama T, Yoshida K.
    • Organizer
      The 84th Annual Meeting of the Physiological Society of Japan. J Physiol Sci
    • Place of Presentation
      Osaka
    • Related Report
      2008 Final Research Report
  • [Presentation] 4-hydroxynonenal(HNE)modifies IgA in rat intestine lipopoly saccharide(LPS) injection2006

    • Author(s)
      Kimura H, Mukaida M, Hashino K, Matsumoto K, Yoshida K.
    • Organizer
      XXth Congress of International Academy of Legal Medicine
    • Place of Presentation
      Budapest
    • Related Report
      2008 Final Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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