Studies on effectiveness of the Chinese medicine against intractable mycobacterial infection
| Project/Area Number |
18590653
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General internal medicine (including Psychosomatic medicine)
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| Research Institution | Shimane University |
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Principal Investigator |
SANO Chiaki Shimane University, Faculty of medicine, Associate professor (70325059)
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| Co-Investigator(Kenkyū-buntansha) |
TOMIOKA Haruaki Shimane university, Faculty of medicine, Professor (40034045)
SHIMIZU Toshiaki Shimane university, Faculty of medicine, Assistant professor (60284030)
TATANO Yutaka Shimane university, Faculty of medicine, Assistant professor (70432614)
YASUMOTO Ko Shimane university, Faculty of medicine, Assistant professor (00448200)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,890,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | oriental medicine / mycobacterial infection / macrophage / antimicrobial effectors / Chinese traditional medicine |
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| Research Abstract |
We examined the effects of immunomodulating agents including various Chinese traditional medicines in potentiating of the host antimicrobial functions against Mycobacterium avium complex (MAC) infection. The following findings were obtained. (1) Mao-Bushi-Saishin-To (MBST) enhanced the macrophage (Mφ) anti-MAC activity. However, forty-five other Chinese traditional medicines did not exhibit such significant effects except for weak effect by Ma-Kyou-Yoku-Kan-To in potentiating anti-MAC activity of host Mφs. Thus, it appears that the efficacy in increasing of the Mφ antimicrobial activity against intracellular MAC by directly augmenting Mφ and/or lymphocyte functions is generally weak in case of most Chinese medicines. (2) The production of reactive oxygen intermediate (ROI) by various types of Mφs were weak induced by MBST treatment except for Kakkon-To. (3) These effects were found only when Mφs were pre-treated with test Chinese traditional medicine in plastic wells. Therefore, it is
… More
likely that there are significant differences in the mode of signal transduction in MBST-activated Mφs which depend on intensity of cell-matrix adhesion. (4) Treatment of Mφs with ATP, which potentiate Mφ anti-MAC activity, in combination with MBST did not significantly increase the production of reactive nitrogen intermediate by Mφs. (5) Concanavalin A (Con A)-induced T cell mitogenic responses were significantly inhibited by Ouren-Gedoku-To and Hochu-Ekki-To, while inhibitory efficacy of MBST was weaker than them. On the other hand, other 17 Chinese traditional medicines did not exhibit such effect on T cell Con A mitogenesis. (6) As the first step in the development of in vitro experimental systems to regulate various Mφ functions by using siRNA, we examined whether Mφ suppressor activity could be controlled by using antisense oligonucleotides specific to PD-L. There was observed the tendency that Mφ suppressor activity was decreased to some extent by the PDL-specific antisense oligonucleotides. (7) We focused on picolinic acid (PA), a natural organic product, as an immunomodulator better than Chinese traditional medicines. We found that PA potentiated Mφ antimicrobial activity against intracellular MAC. In addition, PA induced the early phase apoptotic events in THP-1 Mφs, without inducing DNA fragmentation characteristic to the middle to late phase apoptosis. Less
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Report
(3 results)
Research Products
(134 results)
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Author(s)
Shinizu, Y., Kataoka, S., Aoi, N., Murata, A., Kimura, M., Sano, C., Sano, K., Kawauchi, H
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Journal Title
Allergology in Otolaryngology 26 (1)
Pages: 23-29
NAID
Description
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Author(s)
川内秀之, 片岡真吾, 佐野千晶, 木村光宏, 青井典明, 清水保彦, 梅原 毅, 森倉一朗, 合田 薫, 淵脇貴史, 加藤洋平
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Journal Title
Progress in Medicine 27(11)
Pages: 2615-23
Description
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Peer Reviewed
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[Journal Article] Profiles of Intracellular Expression, Distribution, and Activation of Phospholipase A2 in Host Macrophages Infected with Mycobacterium avium complex2007
Author(s)
Sano, C., Shimizu, T., Tatano, Y., Yasumoto, K., Tomioka, H
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Journal Title
The Journal of the Japanese Association for Infectious Diseases 81 (6)
Pages: 695-699
NAID
Description
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[Journal Article] A clinical trial on the efficacy of single per-os administration of loratacline on perennial allergic rhinitis -Comparison in clinical efficacy between an intake after breakfast and after dinner2007
Author(s)
Kawauchi, H., Kataoka, S., Sano, C., Kimura, M., Aoi, N., Shimizu, Y., Umehara, T., Morikura, I., Goda, K., Fuchiwaki, T., Kato, y
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Journal Title
Progress in Medicine 27 (11)
Pages: 2615-23
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] 通年性アレルギー鼻炎患者を対象としたロラタジンの服用時期の違いによる有用性の検討.2007
Author(s)
川内秀之, 片岡真吾, 佐野千晶, 木村光宏, 青井典明, 清水保彦, 梅原毅, 森倉一朗, 合田 薫, 淵脇貴史, 加藤洋平
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Journal Title
Progress in Medicine 27(11)
Pages: 2615-23
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[Journal Article] Antimicrobial activity of picolinic acid against extracellular and intracellular Mycobacterium avium complex and its combined activity with clarithromycin, rifampicin, and fluoroquinolones2006
Author(s)
Cai, SS., Sato, K., Shimizu, T., Yamabe, S., Hiraki, M., Sano, C., Tomioka, H
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Journal Title
Journal of Antimicrobial Chemotherapy 57 (1)
Pages: 85-93
Description
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[Presentation] Tyrosine dephosphorylation of the aldose reductase in target T cells in terms of suppressor signal transmission concerned with immunosuppressive macrophages2008
Author(s)
Shimizu, T., Tatano, Y., Yasumoto, K., Sato, K., Sano, C., Tomioka, H
Organizer
The 82nd annual meeting of the Japanese association for Infectious diseases
Place of Presentation
Matsue
Year and Date
2008-04-17
Description
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[Presentation] Relationship between the potentiation macrophage antimicrobial activity against intracellular MAC and macrophage apoptosis by picolinic acid or ATP2008
Author(s)
Tatano, Y., Shimizu, T., Yasumoto, K., Sato, K., Akaki, T., Sano, C., Tomioka, H
Organizer
The 82nd annual meeting of the Japanese association for Infectious diseases
Place of Presentation
Matsue
Year and Date
2008-04-17
Description
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[Presentation] Immunomodurating effects of the anti-allergic medicine Mast cells suppress cytokine production2008
Author(s)
Kawauchi, H., Aoi, N., Shimizu, Y., Sano, C., Goda, K., Kataoka, S., Yamada, T
Organizer
The 26th Japan conference of immunology and allergology
Place of Presentation
Osaka
Year and Date
2008-02-22
Description
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[Presentation] Analysis on T cell memory and cytokine production in nasal mucosa2008
Author(s)
Tongu, M., Yamada, T., Aoi, N., Sano, C., Kataoka, S., Kawauchi, H
Organizer
The 26th Japan conference of immunology and allergology
Place of Presentation
Osaka
Year and Date
2008-02-22
Description
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[Presentation] Effects of various immunomodulators on the antimicrobial activity of clarithromycin in combination with rifampin against Mycobacterium avium complex within macrophages2006
Author(s)
Tomioka, H., Shimizu, T., Sano, C., Yamabe, S., Hiraki, M
Organizer
Cytokine 2006 Joint Conference of the ISICR
Place of Presentation
Vienna, Austria
Year and Date
2006-08-30
Description
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[Presentation] Effects of Chinese traditional medicines, chitin, and chitosan on therapeutic efficacy of antimicrobial agents against MAC in macrophage2006
Author(s)
Sato, K., Shimizu, T., Sano, C., Yamabe, S., Tomioka, H
Organizer
The 81st annual meeting of the Japanese society for tuberculosis
Place of Presentation
Sendai
Year and Date
2006-04-28
Description
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清水利朗, 佐藤勝昌, 冨岡治明
Organizer
第81回日本結核病学会総会(シンポジウム)
Place of Presentation
仙台
Year and Date
2006-04-27
Description
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