| Project/Area Number |
18590674
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Hamamatsu University School of Medicine (2007)
Hamamatsu University (2006) |
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Principal Investigator |
KANAOKA Shigeru Hamamatsu University School of Medicine, Faculty of Medicine, Professor (00252172)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Ken-ichi Hamamatsu University School of Medicine, Faculty of Medicine, Assistant Professor (90397430)
HAMAYA Yasushi Hamamatsu University School of Medicine, Faculty of Medicine, Assistant Professor (20436968)
SUGIMOTO Mitsushige Hamamatsu University School of Medicine, Faculty of Medicine, Assistant Professor (80397398)
IKUMA Mutsuhiro Hamamatsu University School of Medicine, Hospital, Assistant Professor (00275108)
OSAWA Satoshi Hamamatsu University School of Medicine, Hospital, Assistant Professor (10397391)
高井 哲成 浜松医科大学, 医学部, リサーチアシスタント (70422748)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,820,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Coloredal enncer / Feces / FecalRNA Test |
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| Research Abstract |
We aimed to promote fecal RNA test (FRT) instead of fecal occult blood test (FOBT) for detecting colorectal cancer. We performed a blind case-control study to prove this test through selecting cyclooxygenase 2 (COX-2) matrix metalloproteinase 7 (MMP-7) and Snail as target biomarkers. We also examined to shorten assay time by real-time PCR instead of conventional PCR. At first the examiner A optimized PCR conditions using fecal samples 15 control cases with no tumors and intlammatuy lesions of colon or rectum unblended to clinical information, the examiner B analyzed 68 cases with colorectal cancer and 15 other control cases by nested PCR blinded to clinical information. The each sensitivity of COX-2, MMP-7 and Snail for cancer was 85%, 65% and 50%, while that of these markers for advanced adenoma was 57%, 50% and 57%. The each sensitivity of FRT, a positive result was defined as being positive at least one marker, for cancer and adenoma was 88% and 86%, while that of a single FOBT was 73% and 36% (P < 0.05, P < 0.05). This study proved that FRT has high sensitivity and high specificity.
We performed the FRT by real-time PCR comparison with the FRT by nested PCR. 'lb optimize real-time PCR conditions, assay time of real-time PCR was reduced to half compared with that of nested PCR. The study cohort included 68 patients with CRC and 30 control patients without colorectal neoplasia. The each sensitivity of COX-2, MMP-7 and Snail for cancer was 85%, 62% and 50%, and consequently the sensitivity of FRT was 90%maitaining 100% specificity. The results of the real-time PCR for each marker accorded with those of nested PCR for the same cases. Finally, the ERT by real-time PCR is as useful as the FRT by nested PCR.
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