Clarify the mechanism of colon carcinogenesis by fat intake
| Project/Area Number |
18590691
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Saga University |
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Principal Investigator |
IWAKIRI Ryuichi Saga University, Saga Medical School Dept. of Gastrointestinal Endoscopy, Assistant Professor (70232642)
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| Co-Investigator(Kenkyū-buntansha) |
TODA Shuji Saga University, Saga Medical School Dept. of Pathology, Professor (80188755)
TSUNADA Seuhu Saga University, Saga Medical School Dept. of Gastrointestinal Endoscopy, Assistant Professor (70264158)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,790,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Colon cancer / azoxymethane / free fatty acid / aberrant crypt foci / conjugated linoleic acid / triglyiceride / グルタチオン / 共役リノール酸 / aberrant crypt foci / 飽和脂肪酸 / 不飽和脂肪酸 |
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| Research Abstract |
The aim of this study is to investigate the effect of conjugated linoleic acid (CLA) on colon carcinogenesis in rats fed with a high fat diet. Materials and Methods: Fifteen mg/kg body weight of azoxymethane (AOM) was i.p injected to male SD rats once a week for 2 weeks. Rats were fed with 10% beef tallow as a basal diet or supplemented with 1% CLA free fatty acid type (FFA) and triglyceride type (TG). Colonic aberrant crypt foci (ACF) was examined at 12 weeks. Colon cancer was examined at 44 weeks. Lipid peroxide level in serum was evaluated by thiobarbituric acid-reactive substance. Superoxide dismutase (SOD) activity was by WST-1 method. Prostaglandin E2 (PGE2) and thromboxane B2 (TXB2) were by EIA. Colon proliferation was evaluated by BrdU uptake. Results: Both of CLA types decreased the number of ACFs and colon cancer incidence. CLA decreased BrdU uptake and expression of PGE2 and TXB2 in colonic mucosa. In rats fed with CLA without AOM, more BrdU uptook cells presented in lower area of crypt. Without CLA, more BrdU uptook cells presented in middle. Only FFA decreased oxidative stress. Conclusion: Despite long term feeding of a high fat diet, ingested CLA especially FFA was suggested to suppress colon carcinogenesis via arachidonate cascade. Decreasing and lower localization of BrdU uptook cells with CLA indicated that CLA itself played an important role in colon carcinogenicity. Furthermore, FFA was suggested to have higher efficacy than TG by decreasing oxidative stress.
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Report
(3 results)
Research Products
(21 results)
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[Journal Article] Suppression of intestinal mucosal apoptosis by ghrelin in fasted rats2008
Author(s)
Park, JM., Kakimoto, T., Kuroki, T., Shiraishi, R., Fujise, T., Iwakiri, R., Fujimoto, K
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Journal Title
Exp Biol Med 233
Pages: 48-56
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Adipocytes and preadipocytes promote the proliferation of colon cancer cells in vitro2007
Author(s)
memori, S., Ootani, A., Aoki, S., Fujise, T., Shimoda, R., Kakimoto, T., Shiraishi, R., Sakata, Y., Tsunada, S., Iwakiri, R., Fujimoto, K
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Journal Title
Am J Physiol Gastrointest Liver Physiol 292
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Long-term feeding of various fat diets modulates azoxymethane-induced colon carcinogenesis through Wnt/{beta}-catenin signaling in rats2007
Author(s)
Fujise, T., Iwakiri, R., Kakimoto, T., Shiraishi, R., Sakata, Y., Wu, B., Tsunada, S., Ootani, A., Fujimoto, K
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Journal Title
Am J Physiol Gastrointest Liver Physiol 292
Description
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[Journal Article] Differentiation of Gastric Surface Mucous Cells(GSM06) Induced by Air Liquid Interface Is Regulated Partly through Mitogen-Activated Protein Kinase Pathway2007
Author(s)
Yokoyama, F., Sakata, Y., Ootani, A., Fujise, T., Kakimoto, T., Amemori, S., Kuroki, T., Tsunada, S., Iwakiri, R., Fujimoto, K
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Journal Title
J. Gastroenterol. Hepatol 22
Pages: 2310-2315
Description
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[Journal Article] Apoptotic pathway in the rat small intestinal mucosa is different between fasting and ischemia-reperfusion.2006
Author(s)
Fujise, T., Iwakiri, R., Wu, B., Amemori, S., Kakimoto, T., Yokoyama, F., Sakata, Y., Tsunada, S., Fujimoto, K
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Journal Title
Am J Physiol Gastrointest Liver Physiol 291
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Mechanism of colon carcinogenesis induced by dietary fatty acid and accelerating Wnt signaling2007
Author(s)
Shiraishi, R., Fujise, T., Kakimoto, T., Kuroki, T., Yamaguchi, K., Watanabe, K., Ootani, H., Shimoda, R., Tsunada, S., Sakata, H., Iwakiri, R., Fujimoto, K
Organizer
108th Annual Meeting of the American Gastroenterological Association, DDW
Place of Presentation
Washington, DC
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Dietary fatty acid composition and GSH affect rat colon carcinogenesis2007
Author(s)
Shiraishi, R., Fujise, T., Kakimoto, T., Kuroki, T., Yamaguchi, K., Watanabe, K., Ootani, H., Shimoda, R., Tsunada, S., Sakata, H., Iwakiri, R., Fujimoto, K
Organizer
108th Annual Meeting of the American Gastroenterological Association DDW
Place of Presentation
Washington, DC
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Effects of dietary fatty acid composition on rat colon carcinogenesis2006
Author(s)
Fujise, T., Kakimoto, T., Shiraishi, R., Yamaguchi, K., Amemori, S., Sakata, Y., Tsunada, S., Iwakiri, R., Fujimoto, K
Organizer
107th Annual Meeting of the American Gastroenterological Association
Place of Presentation
Los Angeles
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Increase in carcinogenesis induced by beef tallow and corn oil through Wnt/beta-catenin signaling pathway is attenuated by fish oil and olive oil in the azoxymethane-induced rat colon cancer model2006
Author(s)
Fujise, T., Iwakiri, R., Kakimoto, T., Shiraishi, R., Sakata, Y., Wu, B., Tsunada, S., Ootani, A., Fujimoto, K
Organizer
14th UEGW
Place of Presentation
Berlin
Description
「研究成果報告書概要(欧文)」より
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