• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Defective response to bacteria through lectin-type receptors in dendritic cells from Crohn diseases

Research Project

Project/Area Number 18590701
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionKeio University

Principal Investigator

OKAMOTO Susumu  Keio University, School of Medicine, Assistant Professor (70255446)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,890,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsCrohn / dendritic cells / lectin / sugar chain
Research Abstract

Human peripheral blood monocytes were differentiated into three types of cells, that is, M-CSF-derived macrophages, GM-CSF-macophages, and dendritic cells. First we compared surface markers on these cells among normal controls (NL), ulcerative colitis (UC), and Crohn's disease (CD) patients, but could not demonstrate any difference in DC-SIGN (CD209), CD14, CD33, CD80, CD86, CD83, HLA-DR, CD206. Next, we stimulated these cells to measure cytokines with LPS and/or DC-SIGN ligands such as mannnann, zymosan, and laminarin. Dendritic cells tended to produce more IL-12 and IL-23, but there were no difference with statistical significance among NL, UC, and CD. Some previous reports demonstrated that DC-SIGN ligands could suppress the production of pro-inflammatory cytokines from LPS-stimulated macrophages. However, I could not see the suppressive effect of DC-SIGN ligands in this set of experiments and now I am trying to find optimal conditions.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi