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Development of in vitro NASH model using hepatocyte spheroid.

Research Project

Project/Area Number 18590735
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionOkayama University

Principal Investigator

SHIRAHA Hidenori (2007)  Okayama University, University Hospital, Assistant Professor (40379748)

真治 紀之 (2006)  岡山大学, 医学部・歯学部附属病院, 講師 (70314680)

Co-Investigator(Kenkyū-buntansha) KOIDE Norio  Okayama University, Graduate Schcol of Medicine, Dentistry and Pharmaceutical Science, Professor (20142333)
白羽 英則  岡山大学, 医学部・歯学部附属病院, 助手 (40379748)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,550,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordshepatocellular spheroids / NASH / adipogenesis
Research Abstract

Mouse fibroblast 3T3-L1 was able to be adipogenetic by IBMX and dexamethason under DMEM+FBS condition. We have tried to form spheroid with 3T3-L1. 3T3-L1 did not form spheroid under Williams E medium with insulin and EGF. To solve this problem, we tried two alternative ways. 1. To enhance EGF signaling, we utilized NIH3T3 cells with EGFR. These cells did not form spheroid. 2. To control cell-cell contact, a tumor suppresser gene was introduced into cells. Introduction of RUNX3 cDNA enhanced cell-cell contact of 3T3 cells. E-cadherin expression was increased in RUNX3 transfected cells.increased in RUNX3 transfected cells. While N-cadherin expression was decreased in these cells. RUNX3 introduced 3T3 was able to form spheroid. These cells may be a useful in vitro model for NASH.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (48 results)

All 2008 2007 2006

All Journal Article (34 results) (of which Peer Reviewed: 9 results) Presentation (12 results) Book (2 results)

  • [Journal Article] Hepatitis B Virus DNA in Liver Tissue and Risk for Hepatocarcinogenesis in Patients with Hepatitis C Virus-Related Chronic Liver Disease. A Prospective Study.2008

    • Author(s)
      Obika M, Shinji T, Fujioka SI, Terada R, Ryuko H, Lwin AA, Shiraha H, Koide N
    • Journal Title

      Intervirology 51(1)

      Pages: 59-68

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Hepatitis B Virus DNA in Liver Tissue and Risk for Hepatocarcinogenesis in Patients with Hepatitis C Virus-Related Chronic Liver Disease. A Prospective Study2008

    • Author(s)
      Obika, M., Shinji, T., Fujioka, SI., Terada, R., Ryuko, H., Lwin, AA., Shiraha, H., Koide. N
    • Journal Title

      Intervirology 51-1

      Pages: 59-68

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Hepatitis B Virus DNA in Liver Tissue and Risk for Hepatocarcinogenesis in Patients with Hepatitis C Virus-Related Chronic Liver Disease. A Prospective Study.2008

    • Author(s)
      Obika M, Shinji T, Fujioka SI, Terada R, Ryuko H, Lwin AA, Shiraha H, Koide N.
    • Journal Title

      Intervirology 51(1)

      Pages: 59-68

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cimetidine inhibits epidermal growth factor-induced cell signaling2007

    • Author(s)
      Fujikawa T, Shiraha H, et. al.
    • Journal Title

      J Gastroenterol Hepatol. 22(3)

      Pages: 436-443

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Des-gamma -carboxy prothrombin-promoted vascular endothelial cell proliferation and migration.2007

    • Author(s)
      Fujikawa T, Shiraha H, Ueda N, Takaoka N, Nakanishi Y, Matsuo N, Tanaka S, Nishina SI, Suzuki M, Takaki A, Sakaguchi K, Shiratori Y.
    • Journal Title

      J Biol Chem. 282(12)

      Pages: 8741-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Hepatitis C Virus Genotype Distribution in Myanmar: Predominance of Genotype 6 and Existence of New Genotype 6 Subtype2007

    • Author(s)
      Lwin AA, Shinji T, Koide N, et. al.
    • Journal Title

      Hepatology Research 37(5)

      Pages: 337-45

    • NAID

      10020163893

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Decreased expression of hMLH1 correlates with reduced 5-fluorouracil-mediated apoptosis in colon cancer cells.2007

    • Author(s)
      Fujita H, Shiraha H, et. al.
    • Journal Title

      Oncology Reports 18(5)

      Pages: 59-68

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Oral administration of taurine improves experimental pancreatic fibrosis.2007

    • Author(s)
      Shirahige A, Koide N, et. al.
    • Journal Title

      Journal of Gastroenterology and Hepatology 23(2)

      Pages: 321-327

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] カルボキシラーゼ遺伝子異常と肝癌増殖2007

    • Author(s)
      白羽英則、他
    • Journal Title

      Minophagen Medical Review 52(2)

      Pages: 144-145

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 腫瘍マーカーの分子機能はどこまで解っているのか 異常プロトロンビンの肝細胞癌進展における役割2007

    • Author(s)
      白羽英則、他
    • Journal Title

      日本臨床検査自動化学会会誌 32(2)

      Pages: 164-165

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 【PIVKA-IIの新しい展開】PIVKA-IIの基礎 PIVKA-IIと細胞増殖2007

    • Author(s)
      白羽英則、他
    • Journal Title

      肝・胆・膵 54(4)

      Pages: 477-481

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 生活習慣病と臨床検査 アルコール多飲の線維化マーカー2007

    • Author(s)
      水島孝明, 越智浩二, 小出典男
    • Journal Title

      臨床病理 55(8)

      Pages: 751-757

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Journal Article] Cimetidine inhibits epidermal growth factor-induced cell signaling2007

    • Author(s)
      Fujikawa, T., Shiraha, H., et. al.
    • Journal Title

      Journal of Gastroenterology and Hepatology 22-3

      Pages: 436-43

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Des-gamma -carboxy prothrombin-promoted vascular endothelial cell proliferation and migration2007

    • Author(s)
      Fujikawa, T., Shiraha, H., Ueda, N., Takaoka, N., Nakanishi, Y., Matsuo, N., Tanaka, S., Nishina, SI., Suzuki, M., Takaki, A., Sakaguchi, K., Shiratori, Y
    • Journal Title

      Biol Chem 282-12

      Pages: 8741-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Hepatitis C Virus Genotype Distribution in Myanmar : Predominance of Genotype 6 and Existence of New Genotype 6 Subtype2007

    • Author(s)
      Lwin, AA., Shinji, T., Koide, N., et. al.
    • Journal Title

      Hepatology Research 37-5

      Pages: 337-45

    • NAID

      10020163893

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Decreased expression of hMLH1 correlates with reduced 5-fluorouracil-mediated apoptosis in colon cancer cells2007

    • Author(s)
      Fujita, H., Shiraha, H., et. al.
    • Journal Title

      Oncology Reports 18-5

      Pages: 59-68

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Oral administration of taurine improves experimental pancreatic fibrosis2007

    • Author(s)
      Shirahige, A., Koide, N., et. al.
    • Journal Title

      Journal of Gastroenterology and Hepatology 23-2

      Pages: 321-7

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Variant carvoxylase contributes hepatocellular carcinoma progression (in Japanese)2007

    • Author(s)
      Shiraha, H., et. al.
    • Journal Title

      Minophagen Medical Review 52-2

      Pages: 144-5

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Des-gamma carboxy prothrombin contributes hepatocellular carcinoma progression (in Japanese)2007

    • Author(s)
      Shiraha, H., et. al.
    • Journal Title

      JACLA Journal 32-2

      Pages: 164-5

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Des-gamma carboxy prothrombin and cellproliferation(in Japanese)2007

    • Author(s)
      Shiraha, H., et. al.
    • Journal Title

      Liver Billiary Pancreas 54-4

      Pages: 477-81

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Fibrosis marker for alcohol drinker(in Japanese)2007

    • Author(s)
      Mizushima, T., Ochi, K., Koide, N
    • Journal Title

      Clinical Pathology 55-8

      Pages: 751-7

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] カルボキシラーゼ遺伝子異常と肝癌増殖2007

    • Author(s)
      白羽英則, 他
    • Journal Title

      Minophagen Medical Review 52(2)

      Pages: 144-145

    • Related Report
      2007 Annual Research Report
  • [Journal Article] 腫瘍マーカーの分子機能はどこまで解っているのか 異常プロトロンビンの肝細胞癌進展における役割2007

    • Author(s)
      白羽英則, 他
    • Journal Title

      日本臨床検査自動化学会会誌 32(2)

      Pages: 164-165

    • Related Report
      2007 Annual Research Report
  • [Journal Article] 【PIVKA-IIの新しい展開】 PIVKA-IIの基礎 PIVKA-IIと細胞増殖2007

    • Author(s)
      白羽英則, 他
    • Journal Title

      肝・胆・膵 54(4)

      Pages: 477-481

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Cimetidine inhibits epidermal growth factor-induced cell signaling2007

    • Author(s)
      Fujikawa T, Shiraha H, et al.
    • Journal Title

      J Gastroenterol Hepatol. 22.3

      Pages: 436-443

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Des-gamma -carboxy prothrombin-promoted vascular endothelial cell proliferation and migration.2007

    • Author(s)
      Fujikawa T, Shiraha H, et al.
    • Journal Title

      J Biol Chem. (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Hepatitis C Virus Genotype Distribution in Myanmar : Predominance of Genotype 6 and Existence of New Genotype 6 Subtype2007

    • Author(s)
      Lwin AA, Shinji T, Koide N, et al.
    • Journal Title

      Hepatology Research (印刷中)

    • NAID

      10020163893

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Three Type 6 Hepatitis C Virus Subgroups among Blood Donors in the Yangon Area of Myanmar Are Identified as Subtypes 6m and 6n, and a Novel Subtype by Sequence Analysis of the Core Region.2006

    • Author(s)
      Shinji T, Lwin AA, Koide N, et. al.
    • Journal Title

      Acta Med Okayama. 60(6)

      Pages: 345-349

    • NAID

      120002305493

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] どうする?パニック値 生化学 9.アンモニア濃度高値2006

    • Author(s)
      真治紀之、小出典男
    • Journal Title

      検査と技術 34(6)

      Pages: 550-551

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Three Type 6 Hepatitis C Virus Subgroups among Blood Donors in the Yangon Area of Myanmar Are Identified as Subtypes 6m and 6n, and a Novel Subtype by Sentience Analysis of the Core Reeion2006

    • Author(s)
      Shinji, T., Lwin, AA., Koide, N., et. al.
    • Journal Title

      Acta Med Okayama 60-6

      Pages: 345-9

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] How to deal with high serum ammonia (in Japanese)2006

    • Author(s)
      Shinji, T., Koide, N
    • Journal Title

      Labmedicin and Tequnique 282-12

      Pages: 8741-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Panic value in laboratory data (in Japanese)2006

    • Author(s)
      Shinji, T., Koide, N
    • Journal Title

      Chugai Igaku

      Pages: 999-999

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Three Type 6 Hepatitis C Virus Subgroups among Blood Donors in the Yangon Area of Myanmar Are Identified as Subtypes 6m and 6n, and a Novel Subtype by Sequence Analysis of the Core Region.2006

    • Author(s)
      Shinji T, Lwin AA, Koide N, et al.
    • Journal Title

      Acta Med Okayama. 60.6

      Pages: 345-349

    • NAID

      120002305493

    • Related Report
      2006 Annual Research Report
  • [Journal Article] どうする?パニック値 生化学 9.アンモニア濃度高値2006

    • Author(s)
      真治紀之, 小出典男
    • Journal Title

      検査と技術 34.6

      Pages: 550-551

    • Related Report
      2006 Annual Research Report
  • [Presentation] Vitamin K依存性カルボキシラーゼ活性による肝癌細胞移動能制御2008

    • Author(s)
      白羽英則、他
    • Organizer
      第11回Vitamin K and aging研究会
    • Place of Presentation
      東京
    • Year and Date
      2008-02-16
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Vitamin K dependent carboxylase suppress cell proliferation of hepatocellular carcinoma2008

    • Author(s)
      Shiraha, H., et. al.
    • Organizer
      Meeting for vitamin K and aging
    • Place of Presentation
      Tokyo
    • Year and Date
      2008-02-16
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Vitamin K依存性カルボキジラーゼ活性による肝癌細胞移動能制御2008

    • Author(s)
      白羽 英則、他
    • Organizer
      第11回Vitamin K and aging研究会
    • Place of Presentation
      東京
    • Year and Date
      2008-02-16
    • Related Report
      2007 Annual Research Report
  • [Presentation] カルボキシラーゼの肝癌血管新生における意義2007

    • Author(s)
      白羽英則、他
    • Organizer
      Japan Digestive Disease Week 2007
    • Place of Presentation
      神戸
    • Year and Date
      2007-10-19
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Splice variant of gamma-glutamyl carboxylase contributes progression of hepatocellular carcinoma.2007

    • Author(s)
      Hidenori Shiraha, et. al.
    • Organizer
      日本癌学会第66回総会
    • Place of Presentation
      横浜
    • Year and Date
      2007-10-04
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] RUNX3 inhibits cell migration in hepatocellular carcinoma cell line.2007

    • Author(s)
      Shigetomi Tanaka, Hidenori Shiraha, et. al.
    • Organizer
      日本癌学会第66回総会
    • Place of Presentation
      横浜
    • Year and Date
      2007-10-04
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Splice variant of gamma-glutamyl carboxylase contributes progression of hepatocellular carcinoma2007

    • Author(s)
      Hidenori, Shiraha, et. al.
    • Organizer
      The 66th annual meeting for Japanese Cancer Association
    • Year and Date
      2007-10-04
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] RUNX3 inhibits cell migration in hepatocellular carcinoma cell line2007

    • Author(s)
      Tanaka, S., Shiraha, H
    • Organizer
      The 66th annual meeting for Japanese Cancer Association
    • Year and Date
      2007-10-04
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Des-gamma carboxy prothrombin promotes angiogenesis in hepatocellular carcinoma2007

    • Author(s)
      Shiraha, H., et. al.
    • Organizer
      Japan Digestive Disease Week 2007
    • Place of Presentation
      Kobe
    • Year and Date
      2007-09-19
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Des-gamma-carboxy prothrombin promotes progression of hepatodellular carcinoma2007

    • Author(s)
      Hidenori Shiraha, et. al.
    • Organizer
      35th Congress of the International Society for On codevelopmental Biology and Medicine
    • Place of Presentation
      Prague, Czech Republic
    • Year and Date
      2007-09-15
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Des-gamma-carboxy prothrombin promotes progression of hepatodellular carcinoma2007

    • Author(s)
      Hidenori, Shiraha, et. al.
    • Organizer
      35th Congress of the International Society for Oncodevelopmental Biology and Medicine
    • Place of Presentation
      Prague
    • Year and Date
      2007-09-15
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Des-gamma-carboxy prothrombin promotes progression of hepatodellular carcinoma2007

    • Author(s)
      Hidenori Shiraha, et. al.
    • Organizer
      35th Congress of the International Society for Oncodevelopmental Biology and Medicine
    • Place of Presentation
      Prague, Czech Republic
    • Year and Date
      2007-09-15
    • Related Report
      2007 Annual Research Report
  • [Book] 検査値の見方-付 パニック値・警戒値-2006

    • Author(s)
      中井利昭, 他 編集
    • Total Pages
      999
    • Publisher
      中外医学社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 検査値の見方-付 パニック値・警戒値-2006

    • Author(s)
      中井利昭 他 編集
    • Total Pages
      999
    • Publisher
      中外医学社
    • Related Report
      2006 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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