Development of in vitro NASH model using hepatocyte spheroid.
| Project/Area Number |
18590735
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Okayama University |
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Principal Investigator |
SHIRAHA Hidenori (2007) Okayama University, University Hospital, Assistant Professor (40379748)
真治 紀之 (2006) 岡山大学, 医学部・歯学部附属病院, 講師 (70314680)
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| Co-Investigator(Kenkyū-buntansha) |
KOIDE Norio Okayama University, Graduate Schcol of Medicine, Dentistry and Pharmaceutical Science, Professor (20142333)
白羽 英則 岡山大学, 医学部・歯学部附属病院, 助手 (40379748)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,550,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | hepatocellular spheroids / NASH / adipogenesis |
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| Research Abstract |
Mouse fibroblast 3T3-L1 was able to be adipogenetic by IBMX and dexamethason under DMEM+FBS condition. We have tried to form spheroid with 3T3-L1. 3T3-L1 did not form spheroid under Williams E medium with insulin and EGF. To solve this problem, we tried two alternative ways. 1. To enhance EGF signaling, we utilized NIH3T3 cells with EGFR. These cells did not form spheroid. 2. To control cell-cell contact, a tumor suppresser gene was introduced into cells. Introduction of RUNX3 cDNA enhanced cell-cell contact of 3T3 cells. E-cadherin expression was increased in RUNX3 transfected cells.increased in RUNX3 transfected cells. While N-cadherin expression was decreased in these cells. RUNX3 introduced 3T3 was able to form spheroid. These cells may be a useful in vitro model for NASH.
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Report
(3 results)
Research Products
(48 results)
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[Journal Article] Hepatitis B Virus DNA in Liver Tissue and Risk for Hepatocarcinogenesis in Patients with Hepatitis C Virus-Related Chronic Liver Disease. A Prospective Study2008
Author(s)
Obika, M., Shinji, T., Fujioka, SI., Terada, R., Ryuko, H., Lwin, AA., Shiraha, H., Koide. N
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Journal Title
Intervirology 51-1
Pages: 59-68
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Des-gamma -carboxy prothrombin-promoted vascular endothelial cell proliferation and migration.2007
Author(s)
Fujikawa T, Shiraha H, Ueda N, Takaoka N, Nakanishi Y, Matsuo N, Tanaka S, Nishina SI, Suzuki M, Takaki A, Sakaguchi K, Shiratori Y.
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Journal Title
J Biol Chem. 282(12)
Pages: 8741-8
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] Des-gamma -carboxy prothrombin-promoted vascular endothelial cell proliferation and migration2007
Author(s)
Fujikawa, T., Shiraha, H., Ueda, N., Takaoka, N., Nakanishi, Y., Matsuo, N., Tanaka, S., Nishina, SI., Suzuki, M., Takaki, A., Sakaguchi, K., Shiratori, Y
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Journal Title
Biol Chem 282-12
Pages: 8741-8
Description
「研究成果報告書概要(欧文)」より
Related Report
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