Research Project
Grant-in-Aid for Scientific Research (C)
Mouse fibroblast 3T3-L1 was able to be adipogenetic by IBMX and dexamethason under DMEM+FBS condition. We have tried to form spheroid with 3T3-L1. 3T3-L1 did not form spheroid under Williams E medium with insulin and EGF. To solve this problem, we tried two alternative ways. 1. To enhance EGF signaling, we utilized NIH3T3 cells with EGFR. These cells did not form spheroid. 2. To control cell-cell contact, a tumor suppresser gene was introduced into cells. Introduction of RUNX3 cDNA enhanced cell-cell contact of 3T3 cells. E-cadherin expression was increased in RUNX3 transfected cells.increased in RUNX3 transfected cells. While N-cadherin expression was decreased in these cells. RUNX3 introduced 3T3 was able to form spheroid. These cells may be a useful in vitro model for NASH.
All 2008 2007 2006
All Journal Article (34 results) (of which Peer Reviewed: 9 results) Presentation (12 results) Book (2 results)
Intervirology 51(1)
Pages: 59-68
Intervirology 51-1
J Gastroenterol Hepatol. 22(3)
Pages: 436-443
J Biol Chem. 282(12)
Pages: 8741-8
Hepatology Research 37(5)
Pages: 337-45
10020163893
Oncology Reports 18(5)
Journal of Gastroenterology and Hepatology 23(2)
Pages: 321-327
Minophagen Medical Review 52(2)
Pages: 144-145
日本臨床検査自動化学会会誌 32(2)
Pages: 164-165
肝・胆・膵 54(4)
Pages: 477-481
臨床病理 55(8)
Pages: 751-757
Journal of Gastroenterology and Hepatology 22-3
Pages: 436-43
Biol Chem 282-12
Hepatology Research 37-5
Oncology Reports 18-5
Journal of Gastroenterology and Hepatology 23-2
Pages: 321-7
Minophagen Medical Review 52-2
Pages: 144-5
JACLA Journal 32-2
Pages: 164-5
Liver Billiary Pancreas 54-4
Pages: 477-81
Clinical Pathology 55-8
Pages: 751-7
J Gastroenterol Hepatol. 22.3
J Biol Chem. (印刷中)
Hepatology Research (印刷中)
Acta Med Okayama. 60(6)
Pages: 345-349
120002305493
検査と技術 34(6)
Pages: 550-551
Acta Med Okayama 60-6
Pages: 345-9
Labmedicin and Tequnique 282-12
Chugai Igaku
Pages: 999-999
Acta Med Okayama. 60.6
検査と技術 34.6