Analysis for mechanism of IRES dependent translation and pathogenesis of hepatitis C virus in B cells

• Project/Area Number: 18590749
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Showa University
• Principal Investigator: ITO Takayoshi Showa University, 医学部, 講師 (50317517)
• Co-Investigator(Kenkyū-buntansha): 井廻 道夫 昭和大学・医学部・教授, 医学部, 教授 (70134228)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,100,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥600,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: 内科 / 消化器内科 / 感染症 / ウイルス / HCV / B細胞トロピズム / リンパ増殖性疾患 / IRES依存性RNA翻訳 / C型肝炎ウイルス / B細胞感染

Research Abstract

HCVは肝細胞トロピズム以外にリンパ球系細胞など臓器トロピズムの異なるHCVが存在する。このことが肝外病変発症と関連する可能性がある。本研究ではC型肝炎患者の末梢血リンパ球からB細胞を分離し、B細胞中で複製するHCVをスクリーニングした。また、そのB細胞トロピズムを持つHCVの全塩基配列を解析した。また、B細胞トロピズムを持つHCVの患者内での病原性について検討した。

Research Products

• [Journal Article] Evaluation of Serum B Cell Activating Factor (BAFF/BlyS) as an Immunological Marker for Lymphoproliferative Disorders Associated with Chronic Hepatitis C (2009)
  • Author(s): Uchikoshi M, Ito T, Shimozuma Y, Inokuchi T, Morikawa K, Nozawa H, Shimazaki T and M. Imawari
  • Journal Title: The Showa University Journal of Medical Sciences (in press)
  • Peer Reviewed
• [Journal Article] Infection of B Cells with Hepatitis C Virus for the Development of Lymphoproliferative (2009)
  • Author(s): Inokuchi M, Ito T, Uchikoshi M, Shimozuma Y, Morikawa K, Nozawa H,Shimazaki T,Hiroishi K, Miyakawa Y and Imawari M
  • Journal Title: Disorders in Patients with Chronic Hepatitis C. J Med Virol.81 Pages: 619-627
  • Peer Reviewed
• [Journal Article] Magnitude of CD8+ T-cell responses against hepatitis C virus and severity of hepatitis do not necessarily determine outcomes in acute hepatitis C virus infection (2009)
  • Author(s): Doi H, Hiroishi K, Shimazaki T, Eguchi J, Baba T, Ito T, Matsumura T, Nozawa H, Morikawa K, Ishii S, Hiraide A, Sakaki M, Imawari M
  • Journal Title: Hepatology Research 39 Pages: 256-265
  • NAID: 10025324889
  • Peer Reviewed
• [Journal Article] Infection of B Cells with Hepatitis C Virus for the Development of Lymphoproliferative Disorders in Patients with Chronic Hepatitis C. (2009)
  • Author(s): Inokuchi M, Ito T, Uchikoshi M, Shimozuma Y, Morikawa K, Nozawa H, Shimazaki T, Hiroishi K, Miyakawa Y, Imawari M.
  • Journal Title: Journal of Medical Virology 81 Pages: 619-627
  • Peer Reviewed
• [Journal Article] Magnitude of CD8+ T-cell responses against hepatitis C virus and severity of hepatitis do not necessarily determine outcomes in acute hepatitis C virus infection. (2009)
  • Author(s): Doi H, Hiroishi K, Shimazaki T, Eguchi J, Baba T, Ito T, Matsumura T, Nozawa H, Morikawa K, Ishii S, Hiraide A, Sakaki M, Imawari M.
  • Journal Title: Hepatology Research 39 Pages: 256-265
  • NAID: 10025324889
  • Peer Reviewed
• [Journal Article] Immune responses in hepatitis C virus infection and mechanisms of hepatitis C virus persistence (2008)
  • Author(s): Hiroishi K, Ito T and Imawari M
  • Journal Title: Journal of Gastroenterol Hepatol 23 Pages: 1473-1482
• [Journal Article] Clonal Expansion of B cells is identified in Japanese Patients Infected with Hepatitis C virus (2008)
  • Author(s): Inokuchi T, Ito T, Uchikoshi M, Shimozuma Y, Nozawa H, Shimazaki T and Imawari M
  • Journal Title: The Showa University Journal of Medical Sciences 23 Pages: 29-39
  • Peer Reviewed
• [Journal Article] Intrahepatic status of regulatory T cells in autoimmune liver disease and chronic viral hepatitis (2008)
  • Author(s): Sakaki M, Hiroishi K, Baba T, Ito T, Hirayama Y, Saito K, Tonoike T, Kushima M and Imawari M
  • Journal Title: Hepatology Research 38 Pages: 354-361
  • NAID: 10024147832
  • Peer Reviewed
• [Journal Article] Immune responses in hepatitis C virus infection and mechanisms of hepatitis C virus persistence. (2008)
  • Author(s): Hiroishi K, Ito T, Imawari M.
  • Journal Title: Journal of Gastroenterol Hepatology 23 Pages: 1473-1482
  • Peer Reviewed
• [Journal Article] Clonal Expansion of B cells is identified in Japanese Patients Infected with Hepatitis C virus. (2008)
  • Author(s): Inokuchi M, Ito T, Uchikoshi M, et al.
  • Journal Title: The Showa University Journal of Medical Sciences 20 Pages: 29-39
  • Peer Reviewed
• [Journal Article] Intrahepatic status of regulatory T cells in autoimmune liver disease and chronic viral hepatitis. (2008)
  • Author(s): Sakaki M, Hiroishi K, Baba T, Ito T, Hirayama Y, Saito K, Tonoike T, Kushima M, Imawari M.
  • Journal Title: Hepatology Research 38 Pages: 354-361
  • NAID: 10024147832
  • Peer Reviewed
• [Journal Article] Intrahepatic status of regulatory T cells in autoimmune liver diseases and chronic viral hepatitis (2008)
  • Author(s): Sakaki, K Hiroishi, T Baba, T Ito, et. al.
  • Journal Title: Hepatology Research 38 Pages: 354-361
  • NAID: 10024147832
  • Peer Reviewed
• [Journal Article] Clonal Expansion of B cells is identified in japanese Patients Infected with Hepatitis C Virus
  • Author(s): M Inokuchi, T Ito, M Uchikoshi, et. al.
  • Journal Title: THE SHOWA UNIVERSITY JOURNAL of MEDICAL SCIENCES (in press)
  • Peer Reviewed
• [Presentation] Evaluation of Serum B Cell Activating Factor (BAFF/BlyS) as an Immunological Marker for Lympho proliferative Disorders Associated with Chronic Hepatitis C (2008)
  • Author(s): Uchikoshi M, Ito T, Shimozuma Y, Inokuchi T, Morikawa K, Nozawa H, Shimazaki T and M. Imawari
  • Organizer: 59th Annual Meeting of the American Association for the Study of Liver Disease
  • Place of Presentation: San Francisco
• [Presentation] Infection of B Cells with Hepatitis C Virus for the Development of Lymphoproliferative Disorders in Patients with Chronic Hepatitis C (2008)
  • Author(s): Inokuchi M, Ito T, Uchikoshi M, Shimozuma Y, Nozawa H, Shimazaki T, Hiroishi K, Miyakawa Y and Imawari M
  • Organizer: 59th Annual Meeting of the American Association for the Study of Liver Disease
  • Place of Presentation: San Francisco
• [Presentation] HCV関連リンパ増殖性疾患における血清B細胞増殖因子(BAFF/BLyS) (2008)
  • Author(s): 打越学, 伊藤敬義, 井口桃子, 森川賢一, 下間祐, 井廻道夫
  • Organizer: 第44回日本肝臓学会総会
  • Place of Presentation: 松山
• [Presentation] HCV関連リンパ増殖性疾患における血清B細胞増殖因子(BAFF/BLyS) (2008)
  • Author(s): 打越学, 伊藤敬義, 井口桃子, 森川賢一, 下間祐, 井廻道夫
  • Organizer: 第94回日本消化器病学会総会
  • Place of Presentation: 福岡
• [Presentation] C型慢性肝炎患者におけるB細胞HCV感染とリンパ増殖性疾患発症についての検討 (2008)
  • Author(s): 井口桃子, 伊藤敬義, 森川賢一, 打越学, 野沢妃佐子, 島崎とも江, 井廻道夫
  • Organizer: 第94回日本消化器病学会総会
  • Place of Presentation: 福岡
• [Presentation] Evaluation of Serum B Cell Activating Factor (BAFF/BlyS)as an Immunological Marker for Lymphoproliferative Disorders Associated with Chronic Hepatitis C. (2008)
  • Author(s): Uchikoshi M, Ito T, Shimozuma Y, Inokuchi T, Morikawa K, Nozawa H, Shimazaki T, M, Imawari.
  • Organizer: 59th Annual Meeting of the American Association for the Study of Liver Diseas
  • Place of Presentation: San Francisco, USA
• [Presentation] Infection of B Cells with Hepatitis C Virus for the Development of Lymphoproliferative Disorders in Patients with Chronic Hepatitis C. (2008)
  • Author(s): Inokuchi M, Ito T, Uchikoshi M, Shimozuma Y, Morikawa K, Nozawa H, Shimazaki T, Hiroishi K, Imawari M.
  • Organizer: 59th Annual Meeting of the American Association for the Study of Liver Disease
  • Place of Presentation: San Francisco, USA
• [Presentation] C型慢性肝炎患者におけるB細胞HCV感染と免疫グロブリン超可変領域遺伝子clonality発症の解析 (2007)
  • Author(s): 井口桃子、伊藤敬義、打越学、森川賢一、野沢妃佐子、島崎とも江、井廻道夫
  • Organizer: 第47回京都肝セルバイオロジー研究会
  • Place of Presentation: ホテルオークラ京都
  • Year and Date: 2007-07-14
• [Presentation] C型慢性肝炎患者におけるB細胞のクローン増殖およびHCV関連リンパ増殖性疾患マーカーに対する抗ウィルス療法の効果 (2007)
  • Author(s): 井口 桃子、伊藤 敬義、打越 学, 他
  • Organizer: 第43回日本肝臓学会総会
  • Place of Presentation: 東京
  • Year and Date: 2007-06-01
• [Presentation] C型慢性肝炎患者におけるB細胞HCV感染とHCV関連リンパ増殖性疾患マーカーに対する抗ウイルス療法の効果 (2007)
  • Author(s): 井口桃子、伊藤敬義、打越学、野沢妃佐子、島崎とも江、井廻道夫
  • Organizer: 第43回日本肝臓学会総会、ワークショップ1「肝炎ウイルス研究の進歩」
  • Place of Presentation: ホテルグランパシフィックメリディアン
• [Presentation] C型慢性肝炎患者におけるリンパ球系細胞へのHCV感染及びその病態に及ぼす影響とIFN療法への反応性 (2006)
  • Author(s): 伊藤敬義, 井口桃子, 打越学, 野沢妃佐子, 島崎とも江, 井廻道夫
  • Organizer: 第42回日本肝臓学会総会、シンポジウム1「難治性ウイルス肝炎に対する新しい治療の基礎的臨床的検討」
  • Place of Presentation: 京都
• [Presentation] C型肝炎ウィルス感染者におけるB細胞へのHCV感染とHCV関連clonalityの検討 (2006)
  • Author(s): 井口桃子, 伊藤敬義, 打越学, 野沢妃佐子, 島崎とも江, 井廻道夫
  • Organizer: 第42回日本肝臓学会総会
  • Place of Presentation: 京都