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Development of drug deliumy system for targeting to injured myocardium by a novel glycoside-binding protein

Research Project

Project/Area Number 18590764
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionShinshu University

Principal Investigator

ISE Hirohiko (2007)  Shinshu University, Division of Cardiovascular Sciences, Department of Organ Regeneration, Assistant Professor (10324253)

木下 修 (2006)  信州大学, 大学院医学研究科, 助教授 (50242681)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Masafumi  Division of Cardiovascular Sciences, Department of Organ Regeneration, Associate Professor (40296108)
IKEDA Uichi  Division of Cardiovascular Sciences, Department of Organ Regeneration, Professor (30221063)
伊勢 裕彦  信州大学, 大学院医学研究科, 助手 (10324253)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsDrug deliverv system / lectin / vimentin / desmin / GlcNAc / 糖鎖 / 心筋細胞 / 血管平滑筋細胞
Research Abstract

We investigated a novel lectin that cardiomyocytes and vascular smooth muscle cells (VSMCs) express on cell surface, in order to elucidate the role of carbohydrate-modifications for regeneration and inflammation in cardiovascular diseases and to develop a drug delivery system by using a lectin. We examined the interaction of various glycoside chains with cardiomyocytes and VSMCs and cardiomyocytes by using glycoside-binding assay with various glycoside-bearing polymers-coated dishes. Cardiomyocytes and VSMCs had the interaction with N-acetylglucosamine (GIcNAc) specifically. Moreover, cardiomyocytes and VSMCs took up the GIcNAc-conjugated liposomes. From these results, we assumed that these cells have a GIcNAc-binding lectin on cell surface and tried to identify the lectin. A GIcNAc-recognizing lectin was identified as desmin and vimentin in cardiomyocytes and as vimentin in VSMCs by using two-dimensional electrophoresis and western blotting with GIcNAc-bearing polymer. To confirm whether vimentin and desmin bind to GIcNAc, we examined a binding capacity of vimentin and desmin for GIcNAc by using BIACORE. Vimentin and desmin interacted with GIcNAc-bearing polymer highly and specifically, whereas not glucose- and galactose-bearing polymers. Moreover, to examine whether these protein express on cell surface. we performed double-immunostaining of these cells with anti-vimentin or desmin antibody and fluorescence-conjugated GIcNAc-bearing polymer. We observed that these antibodies and polymer stained on the same region of cell surface by using laser scanning confocal microscopy. From these results, we suggested that as a novel function of vimentin and desmin. these proteins have a binding capacity for GIcNAc and express on surface of cardiomyocytes and vascular smooth muscle cells.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (32 results)

All 2008 2007 2006

All Journal Article (18 results) (of which Peer Reviewed: 12 results) Presentation (13 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Embryonic undifferentiated cells show scattering activity on a surface coated with immobilized E-cadherin.2008

    • Author(s)
      Nagaoka M, Ise H, Harada I, Koshimizu U, Maruyama A, Akaike T
    • Journal Title

      J Cell Biochem 103

      Pages: 296-310

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Bone Marrow-derived Cells Fuse with Hepatic Oval Cells but are not in volved in Hepatic Tumorigenesis in the Choline-deficient Ethionine-supplemented Diet Rat Model.2008

    • Author(s)
      Kubota K, Soeda J, Misawa R, Mihara M, Miwa S, Ise H. Takahashi M, Miyagawa S
    • Journal Title

      Carcinogenesis (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Modulation of mucus production by interleukin-13 receptor alpha in the human airway epithelium.2008

    • Author(s)
      Tanabe T, Fujimoto K, Yasuo M, Tsushima K, Yoshida K, Ise H, Yamaya M
    • Journal Title

      Clin Exp Allergy 38

      Pages: 122-134

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Embryonic undifferentiated cells show scattering activity on a surface coated with immobilized E-cadherin.2008

    • Author(s)
      NagaoKa M, Ise H, Harada I, Koshimizu U, Maruyama A, Akaike T
    • Journal Title

      J Cell Biochem 103

      Pages: 296-310

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bone Marrow-derived Cells Fuse with Hepatic Oval Cells but are not in volved in Hepatic Tumorigenesis in the Choline-deficient Ethionine-supplemented Diet Rat Model.2008

    • Author(s)
      Kubota K, Soeda J, Misawa R, Mihara M, Miwa S, Ise H, Takahashi M, Miyagawa S
    • Journal Title

      Carcinogenesis (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effective uptake of N-acetylglucosamine-conjugated liposomes by cardiomyocytes in vitro.2007

    • Author(s)
      Aso S, Ise H(Corresponding author), Takahashi M, Kobayashi S, Morimoto H, Izawa A, Goto M, Ikeda
    • Journal Title

      J Control Release 122

      Pages: 189-198

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A novel sphingosine-l-phosphate receptor agonist KRP-203 attenuates rat autoimmune myocarditis.2007

    • Author(s)
      Ogawa R, Takahashi M, Hirose S, Morimoto H, Ise H, Murakami T, Yasue T, Kuriyama K, Hongo M, Kobayashi E, Ikeda U
    • Journal Title

      Biochem Biophys Res Commun 361

      Pages: 621-628

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] one marrow-derived CXCR4+ cells mobilized by macrophage colony-stimulating factor participate in the reduction of infarct area and improvement of cardiac remodeling after myocardial infarction in mice.2007

    • Author(s)
      Morimoto H, Takahashi M, Shiba Y, Izawa A, Ise H, Hongo M, Hatake K, Motoyoshi K, Ikeda U
    • Journal Title

      Am J Pathol 171

      Pages: 755-766

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effective uptake of N-acetylglucosamine-conjugated liposomes by cardiomyocytes in vitro2007

    • Author(s)
      Aso, S., Ise, H., (Correspondina author). Takahashi, M., Kobayashi, S., Morimoto,H., Izawa, A., Goto, M., Ikeda
    • Journal Title

      J. Control Release 122

      Pages: 189-198

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effective uptake of N-acetylglucosamine-conjugated liposomes by cardiomyocytes in vitro.2007

    • Author(s)
      Aso S, lse H(Corresponding author), Takahashi M, Kobayashi S, Morimoto H, Izawa A, Goto M, Ikeda
    • Journal Title

      J Control Release 122

      Pages: 189-198

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel sphingosine-1-phosphate receptor agonist KRP-203 attenuates rat autoimmune myocarditis.2007

    • Author(s)
      Ogawa R, Takahashi M, Hirose S, Morimoto H, Ise H, Murakami T, Yasue T, Kuriyama K, Hongo M, Kobayashi E, Ikeda U
    • Journal Title

      Biochem Biophys Res Commun 361

      Pages: 621-628

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] one marrow-derived CXCR4+ cells mobilized by macrophage colony-stimulating factor participate in the reduction of infarct area and improvement of cardiac remodeling after myocardial infarction in mice.2007

    • Author(s)
      Morimoto H, TakahashiM, Shiba Y, Izawa A, Ise H, Hongo M, Hatake K, Motoyoshi K, Ikeda U
    • Journal Title

      Am J Pathol 171

      Pages: 755-766

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of liver regenerative therapy using glycoside-modified bone marrow cells.2006

    • Author(s)
      Misawa R, Ise H(Corresponding author), Takahashi M, Morimoto H, Kobayashi E, Miyagawa S, Ikeda U. 342, 434-440(2006)
    • Journal Title

      Biochem Biophys Res Commun 342

      Pages: 434-440

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Development of liver regenerative therapy using glycoside-modified bone marrow cells2006

    • Author(s)
      Misawa, R., Ise, H., (Corresponding author). Takahashi, M., Morimoto, H., Kobayashi, E., Miyagawa, S., Ikeda, U
    • Journal Title

      Biochem Biophys Res Commun 342

      Pages: 434-440

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Development of liver regenerative therapy using glycoside-modifies bone marrow cells2006

    • Author(s)
      Misawa R
    • Journal Title

      Biochem Biophys Res Commun 342

      Pages: 432-440

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Collagen synthesis is required for ascorbic acid-enhanced differentiation of mouse embryonic stem cells into cardiomyocytes2006

    • Author(s)
      Sato H
    • Journal Title

      Biochem Biophys Res Commun 342

      Pages: 107-112

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Cardiac overexpression of MCP-1 in transgenic mice prevents cardiac dysfunction and remodeling after myocardial infarction2006

    • Author(s)
      Morimoto H
    • Journal Title

      Circ Res 99

      Pages: 891-899

    • Related Report
      2006 Annual Research Report
  • [Journal Article] G-CSF Accelerates Reendothelialization and Reduces Neointimal Formation After Vascular Injury in Mice2006

    • Author(s)
      Yoshioka T
    • Journal Title

      Cardiovasc Res 70

      Pages: 61-69

    • Related Report
      2006 Annual Research Report
  • [Presentation] 人工的な糖鎖修飾を行った骨髄細胞の心筋組織へのターゲッティング法の開発2008

    • Author(s)
      小林 聡, 伊勢裕彦, 他
    • Organizer
      第7回日本再生医療学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-03-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 心血管傷害部位を標的としたN-acetylglucosamine結合リポソームによる薬物輸送システムの開発2007

    • Author(s)
      伊勢裕彦, 他
    • Organizer
      第29回日本バイオマテリアル学会大会
    • Place of Presentation
      大阪
    • Year and Date
      2007-11-26
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] ラット心筋細胞における新規糖鎖結合分子の探索2007

    • Author(s)
      小林 聡, 伊勢裕彦, 他
    • Organizer
      第27回日本糖質学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2007-08-01
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Vimentin and desmin have a binding capacity for N-acetylglucosa mine and are expressed on surface of cardiomyocytes and vascular smooth muscle cells2007

    • Author(s)
      Ise H, 他
    • Organizer
      第59回 日本細胞生物学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2007-05-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Vimentin and desmin have a binding capacity for N-acetylglucosamine and are expressed on surface of cardiomyocytes and vascular smooth muscle cells2007

    • Author(s)
      Ise H, 他
    • Organizer
      第59回 日本細胞生物学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2007-05-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] Targeted drug delivery to vascular smooth muscle cells using glycoside-conjugated liposomes.2007

    • Author(s)
      Ise H, 他
    • Organizer
      第71回日本循環器学会総会
    • Place of Presentation
      神戸
    • Year and Date
      2007-03-17
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Targeted drug delivery to vascular smooth muscle cells using glycoside-conjugated liposomes2007

    • Author(s)
      Ise, H
    • Organizer
      The 71th annual scientific meeting of the Japanese circulation Society
    • Place of Presentation
      Kobe
    • Year and Date
      2007-03-17
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Targeted drug delivery to the injured myocardium using glycoside-conjugated liposomes.2007

    • Author(s)
      Aso S, Ise H, 他
    • Organizer
      第71回日本循環器学会総会
    • Place of Presentation
      神戸
    • Year and Date
      2007-03-15
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Targeted drug delivery to the injured myocardium using glycoside-conjugated liposomes2007

    • Author(s)
      Aso, S., Ise, H
    • Organizer
      The 71th annual scientific meeting of the Japanese circulation Society
    • Place of Presentation
      Kobe
    • Year and Date
      2007-03-15
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 糖鎖結合リポソームを用いた心筋細胞を標的とする薬物輸送システムの開発2007

    • Author(s)
      小林 聡, 伊勢裕彦, 他
    • Organizer
      第6回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2007-03-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ラット心筋細胞に発現する新規糖鎖結合分子の探索2007

    • Author(s)
      小林 聡, 伊勢裕彦, 他
    • Organizer
      第6回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2007-03-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Drug delivery system targeting the injured myocardium.2006

    • Author(s)
      Aso S, Ise H, 他
    • Organizer
      American Heart Association Scientific Sessions 2006
    • Place of Presentation
      シカゴ
    • Year and Date
      2006-11-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Drug delivery system targeting the injured myocardium2006

    • Author(s)
      Aso, S., Ise, H
    • Organizer
      American Heart Association Scientific Sessions 2006
    • Place of Presentation
      Chicago
    • Year and Date
      2006-11-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] N-アセチルグルコサミン糖鎖認識タンパク質2007

    • Inventor(s)
      伊勢裕彦, 他
    • Industrial Property Rights Holder
      信州大学
    • Industrial Property Number
      2007-213039
    • Filing Date
      2007-08-17
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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