Low invasive therapy with gratnilotyte colony stimulating factor in patients with coronary artery dicease
| Project/Area Number |
18590765
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Gifu University |
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Principal Investigator |
MINATOGUCHI Shinya Gifu University, Graduate Schcol of Medicine, Professor (20190697)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,890,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | G-CSF / cardiac function / pravastatin / NO / free radicals / 心筋梗塞 / 梗塞サイズ / ハイドロキシラジカル / スーパーオキシド / pravastatin / 心筋梗塞サイズ / 一酸化窒素 |
|---|
| Research Abstract |
1) In patients with acute myocardial infarction, subcutaneous treatment with G-CSF at a dose of 2-5 mg/kg significantly improved ejection fraction at 1 months after myocardial infarction assacsed by left ventriculogram (Suzuki K, et. al. Circ J 2006)
2) Rabbits underwent 30 min of coronary occlusion followed by 48 hours of reperfusion Pravastatin (1 or 5mg/kg) or saline was intravenously administered 10 min before ischemia. Pravastatin (5mg/kg) was also administered 10 min before reperfusion. L-NAME (10mg/kg), chelerythrine (5mg/kg) or 5-HD (5mg/kg) was i.v. administered 10 min before pravastatin injection. The infarct size was determined. The myocardial interstitial levels of 2, 5-DHBA and NOx, and the intensity of myocardial dihydroethidium staining were obtained. Pre-ischemic treatment with pravastatin reduced the infarct size (34±5% and 24±4%, 1 and 5mg/kg, respectively) but not pre-reperfusion treatment (42.1±3.7%) compared with the control (45±3%). This effect was blocked by L-NAME (42.6±4%), chelerythrine (50.9±3%) and 5-HD (52.7±2%), respectively. Pre-ischemic treatment with pravastatin increased myocardial NOx levels and attenuated the 2,5-DHBA levels and the intensity of dihydroethidium staining during reperfusion. Chelerythrine abolished the increase in NOx levels by pravastatin. Pre-ischemic treatment with pravastatin reduces the myocardial infarct size via protein kinase C-dependent nitric oxide production, decreasing hydroxyl radicals and superoxide, and opening the mitochondrial KATP channels.
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Report
(3 results)
Research Products
(7 results)
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[Journal Article] Pravastatin reduces myocardial infarct size via increasing protein kinase C-dependent nitric oxide, decreasing oxyradicals and opening the mitochondrial adenosine triphosphate-sensitive potassium channels in rabbits2007
Author(s)
Bao, N., Minatoguchi, S., Kobayashi, H., Yasuda, S., Kawamura, I., Iwasa, M., Yamaki, T., Sumi, S., Misao, Y., Arai, M, Nishigaki, K., Takemura, G., Fujiwara, T., Fujiwara, H
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Journal Title
Circ J 71
Pages: 1622-1628
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Effect of granulocyte colony-stimulating factor treatment at a low dose but for a long duration in patients with coronary heart disease -a pilot study2006
Author(s)
Suzuki, K., Nagashima, K., Arai, M, Uno, Y., Misao, Y., Takemura, G., Nishigaki, X, Minatoguchi, S., Watanabe, S., Tei, C., Fujiwara, H
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Journal Title
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] A low dose but long duration post-myocardial infarction treatment with granulocyte colony-stimulating factor shows a beneficial effect without any complications2008
Author(s)
Minatoguchi, S., Arai, M., Suzuki, K., Nishigaki, K., Takemura, G., Fujiwara, H
Organizer
The 72nd Annual Scientific Meeting of the Japanese Circulation Society
Place of Presentation
Fukuoka, Japan
Year and Date
2008-03-30
Description
「研究成果報告書概要(欧文)」より
Related Report
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