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Molecular mechanism for intercellular interactions involved in cardiovascular a〓craniofacial development

Research Project

Project/Area Number 18590802
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionThe University of Tokyo

Principal Investigator

KURIHARA Yukiko  The University of Tokyo, Graduate School of Medicine, Assistant Professor (80345040)

Co-Investigator(Kenkyū-buntansha) KURIHARA Hiroki  The University of Tokyo, Graduate School of Medicine, Professor (20221947)
天野 朋和  東京大学, 大学院医学系研究科, 寄付講座教員(助手相当) (50359634)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsEndothelin / Knock-In mouse / Cardiovascular development / Morphogenesis / 神経堤細胞
Research Abstract

Cardiovascular and craniofacial development in vertebrates is a complex process involving the coordinated interaction of different cell populations. To elucidate how cell-cell interactions operate cell differentiation and organogenesis, cardiovascular and craniofacial development serves as a good model. Our previous study has revealed that Endothelin-1/Endothelin A receptor (ETAR) signal was essential for cranial and cardiac neural crest cell-derived tissue development(Nature 368, 703, 1994). In this study, we have established ETAR knock-in mice in which genes of interested can be expressed under the ETAR promoter by recombinase mediated cassette exchange (RMCE), and have used this system for analysis as follows.
1. Knock-in of ETAR cDNA The ETAR-null phenotype was completely rescued in ETAR-/cETAR mice. Cardiovascular and craniofacial abnormalities were normalized, suggesting that this RMCE system functioned well in the ETAR gene.
2. Knock-in of marker genes The lacZ expression in ETAR+/lacZ mice almost recapitulates the expression of ETAR mRNA revealed by whole-mount in situ hybridization. Moreover, ETAR expressing cells except for neural crest cells were i
3. Knock-in of ETAR-/ETBR ETAR has been considered to be largely equivalent to ETBR in terms of Gq/11-mediated signaling in in
4. Knock-in of other genes We are establishing mice in terms of Gq/11-mediated signaling in which ETARs are ubiquitously activate

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (28 results)

All 2008 2007 2006 Other

All Journal Article (19 results) (of which Peer Reviewed: 10 results) Presentation (8 results) Remarks (1 results)

  • [Journal Article] Maintenance of genomic methylation patterns during preimplantation development requires the somatic form of DNA methyltransferase 1.2008

    • Author(s)
      Kurihara, Y.
    • Journal Title

      Dev. Biol. 313

      Pages: 335-346

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Recombinase-mediated cassette exchange revealed the selective use of Gq/Gll-dependent and-independent endothelin-I/endothelin type-A receptor signaling in pharyngeal arch development.2008

    • Author(s)
      Sato, T.
    • Journal Title

      Development 135

      Pages: 755-765

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Multiple renal cysts, urinary concentration defects, and pulmonary emphysematous changes inmice lacking TAZ.2008

    • Author(s)
      Makita, R.
    • Journal Title

      Am J Physiol Renal Physiol. 294

      Pages: 542-553

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Maintenance of genomic methylation patterns during preimplantation development requires the somatic form of DNA methyl transferase 12008

    • Author(s)
      Kurihara, Y.
    • Journal Title

      Dev. Biol 313

      Pages: 335-346

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Recombinase-mediated cassette exchange revealed the selective use of Gq/G11-dependent and -independent endothelin-1/endothelin type-A receptor signaling in pharyngeal arch development2008

    • Author(s)
      Sato, T.
    • Journal Title

      Development 135

      Pages: 755-765

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Recombinase-mediated cassette exchange revealed the selective use of Gq/G11-dependent and-independent endothelin-1/endothelin type-A receptor signaling in pharyngeal arch development.2008

    • Author(s)
      Sato, T.
    • Journal Title

      Development 135

      Pages: 755-765

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multiple renal cysts, urinary concentration defects, and pulmonary emphysematous changes in mice lacking TAZ.2008

    • Author(s)
      Makita, R.
    • Journal Title

      Am J Physiol Renal Physiol. 294

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protein kinase A-regulated nucleocytoplasmic shuttling of Idl during angiogenesis.2007

    • Author(s)
      Nishiyama, K.
    • Journal Title

      J Biol Chem. 282

      Pages: 17200-17209

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Calpain 6 is involved in microtubule stabilization and cytoskeletal organization.2007

    • Author(s)
      Tonami, K.
    • Journal Title

      Mol Cell Biol 27

      Pages: 2548-2561

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Molecular dynamics of retinoic acid-induced craniofacial malformations: implications for the origin of gnathostome jaws.2007

    • Author(s)
      Vieux-Rochas, M.
    • Journal Title

      PLoS ONE 2

      Pages: 510-510

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Calcitonin gene-related peptide mediates acid-induced lung injury in mice.2007

    • Author(s)
      Aoki-Nagase, T.
    • Journal Title

      Respirology 12

      Pages: 807-813

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Protein kinase A-regulated nucleocytoplasmic shuttling of Id1 during angiogenesis2007

    • Author(s)
      Nishiyama, K.
    • Journal Title

      J Biol Chem 282

      Pages: 17200-17209

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Calpain 6 is involved in microtubule stabilization and cytoskeletal organization2007

    • Author(s)
      Tonami, K.
    • Journal Title

      Mol Cell Biol 27

      Pages: 2548-2561

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Molecular dynamics of retinoic acid-induced craniofacial malformations : implications for the origin of gnathostome jaws2007

    • Author(s)
      Vieux-Rochas, M.
    • Journal Title

      PLoS ONE 2

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Calcitonin gene-related peptide mediates acid-induced lung in jury in mice2007

    • Author(s)
      Aoki-Nagase, T.
    • Journal Title

      Respirology 12

      Pages: 807-813

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Multiple renal cysts, urinary concentration defects, and pulmonary emphysematous changes in mice lacking TAZ2007

    • Author(s)
      Makita, R.
    • Journal Title

      Am J Physiol Renal Physiol 294

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Protein kinase A-regulated nucleocytoplasmic shuttling of Id1 during angiogenesis.2007

    • Author(s)
      Nishiyama, K.
    • Journal Title

      J Biol Chem. 282

      Pages: 17200-17209

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protein kinase A-regulated uncleocytoplasmic shuttling of Idl during angiogenesis2007

    • Author(s)
      Nishiyama K
    • Journal Title

      J Biol Chem (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Calpain 6 is involved in microtubule stabilization and cytoskeletal organization2007

    • Author(s)
      Tonami K
    • Journal Title

      Mol Cell Biol 27・7

      Pages: 2548-2461

    • Related Report
      2006 Annual Research Report
  • [Presentation] Dnmtlsによる着床前胚のDNAメチル化維持機構2007

    • Author(s)
      栗原由紀子
    • Organizer
      第30回日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-12
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Maintenance of genomicmethylation during preimplantation development is regulated by the somatic form of DNA methyltransferase 12007

    • Author(s)
      Kurihara, Y.
    • Organizer
      Joint Annual Meeting of the Molecular Biology Society of Japan and the Japanese Biochemical
    • Place of Presentation
      Yokohama
    • Year and Date
      2007-12-12
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Dnmtlsによる着床前胚のDNAメチル化維持機構2007

    • Author(s)
      栗原由紀子
    • Organizer
      日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] DNMT1 s in Preimplantation Embryo2007

    • Author(s)
      栗原由紀子
    • Organizer
      第40回日本発生生物学会
    • Place of Presentation
      福岡
    • Year and Date
      2007-05-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] DNMT1s in Preimplantation Embryo2007

    • Author(s)
      Kurihara, Y.
    • Organizer
      Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      Fukuoka
    • Year and Date
      2007-05-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] DNMTIs in Preimplantation Embryo2007

    • Author(s)
      栗原由紀子
    • Organizer
      日本発生生物学会
    • Place of Presentation
      福岡
    • Year and Date
      2007-05-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] マウス着床前胚におけるDNAメチル化維持機構の解明2006

    • Author(s)
      栗原由紀子
    • Organizer
      第4回幹細胞シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2006-05-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Maintenance of genomicmethylation during preimplantation development is regulated by the somatic form of DNA methyltransferase 12006

    • Author(s)
      Kurihara, Y.
    • Organizer
      Stem Cell Research Symposium
    • Place of Presentation
      Tokyo
    • Year and Date
      2006-05-20
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://bio.m.u-tokyo.ac.jp/home-j.html

    • Related Report
      2007 Final Research Report Summary

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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