Analysis of the function of endothelial ROCK1 in pulmonary hypertension
| Project/Area Number |
18590812
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Kobe University |
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Principal Investigator |
RIKITAKE Yoshiyuki Kobe University, Graduate Schcol of Medicine, Assistant Professor (50419488)
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| Co-Investigator(Kenkyū-buntansha) |
横山 光宏 神戸大学, 医学系研究科, 教授 (40135794)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,910,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | vascular endothelial cell / ROCKI / signal transduction |
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| Research Abstract |
I investigated to analyze the roles of ROCK1 in regulation mechanism of vascular diseases by ROCK1 and obtained the following results.
1. The role of ROCK1 in vascular remodeling was analyzed using mouse common carotid ligation model. Intimal thickening and stenosis in carotid arteries in ROCK1+/- mice were reduced as compared to those in wild-type mice.
2. Vascular inflammation and DNA synthesis of vascular wall cells after carotid ligation in ROCK1+/- mice were reduced as compared to those in wild-type mice.
3. Similarly to ROCK1+/- mice, reduced vascular remodeling was observed in wild-type mice received bone marrow transplantation of ROCK1+/- mice.
4. Vascular endothelial cells primary cultured from ROCK1+/- mice showed reduced ROCK activity and inhibition of adhesion molecule expression as compared to those from wild-type mice.
5. Vascular smooth muscle cells obtained from ROCK1+/- mice showed reduced ROCK activity and inhibition of cell migration as compared to those from wild-type mice.
6. Inhibition of ROCK reduced maturation of focal complex to focal adhesion and cell movement.
7. Inhibition of ROCK stimulated BH4 synthesis and thereby increased nitric oxide production in endothelial cells.
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Report
(3 results)
Research Products
(6 results)
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[Journal Article] Roles of Nec1-5/poliovirus receptor and Rho-associated kinase(ROCK)in the regulation of transformation of integrin αVβ3-based focal complexes into focal adhesions
Author(s)
Yuichi, Nagamatsu, Yoshiyuki, Rikitake, Motonori, Takahashi, Yuko, Deki, Wataru, Ikeda, Kenichi, Hirata, Yoshimi, Takai
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Journal Title
Journal of Biological Chemistry (in press)
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Sequential activation of Rap 1 and Rac1 small G proteins by PDGF locally at leading edges of NIH3T3 cells
Author(s)
Motonori, Takahashi, Yoshiyuki, Rikitake, Yuichi, Nagamatsu, Tetsuya, Hara, Wataru, Ikeda, Ken-ichi, Hirata, Yoshimi, Takai
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Journal Title
Genes to Cells (in press)
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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