| Project/Area Number |
18590827
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
TASAKI Hiromi University of Occupational and Environmental Health, Japan, 医学部, 非常勤医師 (60216950)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Kazuhito 産業医科大学, 医学部, 助教 (00341496)
筒井 正人 産業医科大学, 医学部, 准教授 (70309962)
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| Co-Investigator(Renkei-kenkyūsha) |
TSUTSUI Masato 琉球大学, 医学部, 教授 (70309962)
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| Project Period (FY) |
2006 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,950,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2008: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | 肺高血圧症 / スーパーオキシドジスムターゼ / モノクロタリン / 遺伝子治療 / ECSOD / 経気管投与 / 胚高血圧症 |
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| Research Abstract |
In the present study, we examined whether intratracheal gene transfer of human extracellular superoxide dismutase (ECSOD) could ameliorate monocrotaline (MCT)-induced PAH in rats. MCT-injected rats were concomitantly intratracheally administered vehicle (MCT group), an adenovirus expressing β-galactosidase (Adβgal group), or human ECSOD (AdECSOD group). Twenty-eight days after the MCT injection, right ventricular systolic pressure and the weight ratio of the right ventricle to the left ventricle plus septum were significantly lower in the AdECSOD group. As the mechanism of these effects, 8-isoprostane in lung tissue was significantly reduced in the AdECSOD group. ECSOD overexpression to the lung ameliorated MCT-induced PAH in rats.
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