Establishment of the therapeutic strategy for the cardiovascular complications in Metabolic syndrome by gene trap method

• Project/Area Number: 18590828
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: Kyoto University
• Principal Investigator: ONO Koh Kyoto University, Department of Cardiovascular Medicine, Assistant Professor (00359275)
• Co-Investigator(Kenkyū-buntansha): HASEGAWA Koji Kyoto Medical Center, Division of Translational Research, Director (50283594)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: gene-trap / metabolic syndrome / adipocyte / serotonin / cardiomyocyte / GLUT4 / AMPK / TGIF

Research Abstract

The differentiation of preadipocytes into mature adipocytes is closely associated with the expression of adipocyte-specific gene products that are deeply involved in insulin resistance. Therefore, it is critical that we identify the genes required for differentiation into mature adipocytes. We used retrovirus insertion-mediated random mutagenesis to generate 3T3-L1 cell lines which lose their ability to differentiate into mature adipocytes. Using this approach, we discovered that tryptophan hydroxylase-1 (TPH1), a rate-limiting enzyme for the biosynthesis of serotonin, was required for adipocyte differentiation. While differentiation was clearly reduced in a mutant 3T3L-1 cell line that lacked expression of the normal TPH1 gene, reconstitution of the TPH1 gene in this cell line restored its differentiation potential. The administration of serotonin enhanced the differentiation in 3T3-L1 cells and in cells of stromal vascular fraction from adult mice. On the other hand, the serotonin sy nthesis inhibitor p-chlorophenylalanine and 5-hydroxy tryptophan receptor type 2A antagonists inhibited differentiation. Differentiation was significantly impaired in preadipocytes prepared from TPH-/- mice compared to those from wild-type mice. We observed no differences in the histology of adipose tissue between TPH-/- and wild-type mice at the basal state. However, after obesity was induced by a high-fat diet, increases in body weight and mesenteric fat weight were more prominent in TPH1-/- mice than in wild-type mice. Furthermore, glucose tolerance was more prominently impaired in TPH-/- mice than in wild-type mice. Interestingly, in obese groups, we observed extremely large (140μm〜) adipocytes in TPH-/- mice, in contrast to the usually hypertrophied (〜80μm) adipocytes in wild-type mice. We also observed PCNA-positive adipocytes in wild-type mice but not in TPH-/- mice, which suggests that adipocyte production is impaired in TPH-/- mice. Thus, serotonin in adipose tissue acts as an autocrine factor and is involved in adipocyte differentiation. The results also suggest that serotonin is required for the physiological hypertrophy of adipocytes in response to a high-fat diet, and for the preservation of normal glucose tolerance in adult mice.
We also found TG-interacting factor and preproenkephalin-1 are required for the differentiation of preadiopcytes. We are now trying to establish therapeutic strategies against obesity and metabolic syndrome by targeting these molecules.
In hypoxia and metabolic stress condition, cardiomyocytes adapt themselves by utilizing glycolytic pathway for their major energy source. In order to find the signal transduction mechanisms involved in such conditions, we have established a method to measure GLUT4 translocation by flow cytometry and found that both CMKKβ and LKB1 activate AMPK in cardiomyocytes.

Research Products

• [Journal Article] Identification of p300-targeted acetylated residues in GATA-4 during hypertrophic responses in cardiac myocytes. (2008)
  • Author(s): Takaya-T, et. al.
  • Journal Title: J Biol Chem. 283 Pages: 9828-35
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Inhibition of CCN1 suppressed neointimal hyperplasia in a rat ballooninjury model. (2008)
  • Author(s): Matsumae-H, et. al.
  • Journal Title: J Cell Physiol. 215 Pages: 733-42
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Small dense LDL-cholesterol relative to LDL-cholesterol is a strong independent determinant of hypoadiponectinemia in metabolic syndrome. (2008)
  • Author(s): Satoh-N, et. al.
  • Journal Title: J Cell Physiol. 215 Pages: 733-42
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] TG-interacting factor is required for the differentiation of preadipocytes. (2008)
  • Author(s): Horie-T, et. al.
  • Journal Title: J Lipid Res. 49 Pages: 1224-34
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Oxidative stress induces GLUT4 translocation by activation of PI3-K/Akt and dual AMPK kinase in cardiac myocytes. (2008)
  • Author(s): Horie-T, et. al.
  • Journal Title: J Cell Physiol. 215 Pages: 733-42
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Identification of p300-targeted acetylated residues in GATA-4 during hypertrophic responses in cardiac myocytes (2008)
  • Author(s): Takaya, T., Kawamura, T., Morimoto, T., Kita, T., Shimatsu, A., Hasegawa, K
  • Journal Title: J Biol Chem 283(Epub ahead of print) Pages: 9828-35
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Inhibition of CCN1 suppressed neointimal hyperplasia in a rat balloon injury model (2008)
  • Author(s): Matsumae-H., Yoshida-Y., Ono-K., Togi-K., Inoue-K., Furukawa-Y., Nakashima-Y., Kojima-Y., Nobuyoshi-M., Kita-T., Tanaka-M
  • Journal Title: Arterioscler Thromb Vasa Biol 28 Pages: 1077-83
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Small dense LDL-cholesterol relative to LDL-cholesterol is a strong independent determinant of hypoadiponectinemia in metabolic syndrome (2008)
  • Author(s): Satoh-N., Wada-H., Yamakage-H., Yamada-K., Nakano-T., Hattori-M., Shimatsu-A., Kuzuya-H., Hasegawa-K
  • Journal Title: Circulation J (in press)
  • NAID: 110006783830
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] TG-interacting factor is required for the differentiation of preadipocytes (2008)
  • Author(s): Horie-T., Ono-K., Kinoshita-M., Nishi-H., Nagao-K., Kawamura-T., Abe-Y., Wada-H., Shimatsu-A., Kita-T., Hasegawa-K
  • Journal Title: J Lipid Res 49 Pages: 1224-34
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Oxidative stress induces GLUT4 translocation by activation of PI3-K/Akt and dual AMPK kinase in cardiac myocytes (2008)
  • Author(s): Horie-T., Ono-K., Nagao-K., Nishi-H., Kinoshita-M., Kawamura-T., Wada-H., Shimatsu-A., Kita-T., Hasegawa-K
  • Journal Title: J Cell Physiol 215 Pages: 733-42
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Leptin induces elongation of cardiac myocyte and causes eccentric left ventricular dilatation with compensation. (2007)
  • Author(s): Abe-Y, et. al.
  • Journal Title: Am J Physiol Heart Circ Physiol. 292
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Serum adiponectin level as an independent predictor of mortality in patients with congestive heart failure (2007)
  • Author(s): Tamura-T, et. al.
  • Journal Title: Circ J. 71 Pages: 623-630
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Trichostatin A induces myocardial differentiation of monkey ES cells. (2007)
  • Author(s): Hosseinkhani-M, et. al.
  • Journal Title: Biochem Biophys Res Commun. 356 Pages: 386-391
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] CCN1 protects cardiac myocytes from oxidative stress via β1 integrin-Akt pathway. (2007)
  • Author(s): Yoshida-Y, et. al.
  • Journal Title: Biochem Biophys Res Commun 355 Pages: 611-618
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Leptin induces elongation of cardiac myocyte and causes eccentric left ventricular dilatation with compensation (2007)
  • Author(s): Abe, Y., Ono, K., Kawamura, T., Wada, H., Kita, T., Shimatsu, A., Hasegawa, K
  • Journal Title: Am J Physiol Heart Circ Physiol 292 Pages: 2387-96
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Serum adiponectin level as an independent predictor of mortality in patients with congestive heart failure (2007)
  • Author(s): Tamura, T., Furukawa, Y., Taniguchi, R., Sato, Y., Ono, K., Horiuchi, H., Nakagawa, Y., Kita, T., Kimura, T
  • Journal Title: Circ J 71 Pages: 623-30
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Trichostatin A induces myocardial differentiation of monkey ES cells (2007)
  • Author(s): Hosseinkhani, M., Hasegawa, K., Ono, K., Kawamura, T., Takaya, T., Morimoto, T., Wada, H., Shimatsu, A., Prat, SG., Suemori, H., Nakatsuji, N., Kita, T
  • Journal Title: Biochem Biophys Res Commun 356 Pages: 386-91
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] CCN1 protects cardiac myocytes from oxidative stress via 61 integrin輸kt pathway (2007)
  • Author(s): Yoshida, Y., Togi, K., Matsumae, H., Nakashima, Y., Kojima, Y., Yamamoto, Y., Ono, K., Nakamura, T., Kita, T., Tanaka, M
  • Journal Title: Biochem Biophys Res Commun 355 Pages: 611-8
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Serum adiponectin level as an independent predictor of mortality in Patients with congestive heart failure (2007)
  • Author(s): Tamura-T, et. al.
  • Journal Title: Circ J. 71 Pages: 623-630
  • Peer Reviewed
• [Journal Article] CCN1 protects cardiac myocytes from oxidative stress viaβ1 integrin-Akt pathway. (2007)
  • Author(s): Yoshida-Y, et. al.
  • Journal Title: Biochem Biophys Res Commun 355 Pages: 611-618
  • Peer Reviewed
• [Journal Article] Trichostatin A induces myocardial differentiation of monkey ES cells. (2007)
  • Author(s): Hosseinkhani M, Hasegawa K, Ono K, Kawamura T, Takaya T, Morimoto T, Wada H, Shimatsu A, Prat SG, Suemori H, Nakatsuji N, Kita T.
  • Journal Title: Biochem Biophys Res Commun. 356 Pages: 386-391
• [Journal Article] CCN1 protects cardiac myocytes from oxidative stress via β1 integrin-Akt pathway. (2007)
  • Author(s): Yoshida Y, Togi K, Matsumae H, Nakashima Y, Kojima Y, Yamamoto Y, Ono K, Nakamura T, Kita T, Tanaka M.
  • Journal Title: Biochem Biophys Res Commun. 355 Pages: 611-618
• [Journal Article] Leptin induces elongation of cardiac myocyte and causes eccentric left ventricular dilatation with compensation. (2007)
  • Author(s): Abe Y, Ono K, Kawamura T, Wada H, Kita T, Shimatsu A, Hasegawa K.
  • Journal Title: Am J Physiol (in press)
• [Journal Article] Three catheter-based strategies for cardiac delivery of therapeutic gelatin microspheres. (2006)
  • Author(s): Hoshino K, et. al.
  • Journal Title: Gene Ther. 13 Pages: 1320-7
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Histone acetyltransferase activity of p300 is required for the promotion of left ventricular remodeling following myocardial infarction in adult mice in vivo. (2006)
  • Author(s): Miyamoto S, et. al.
  • Journal Title: Circulation. 113 Pages: 679-90
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Three catheter-based strategies for cardiac delivery of therapeutic gelatin microspheres (2006)
  • Author(s): Hoshino, K., Kimura, T., De, Grand, AM., Yoneyama, R., Kawase, Y., Houser, S., Ly, HQ., Kushibiki, T., Furukawa, Y., Ono, K., Tabata, Y., Frangioni, JV., Kita, T., Hajjar, RJ., Hayase, M
  • Journal Title: Gene Ther 13(18) Pages: 1320-7
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Histone acetyltransferase activity of p300 is required for the promotion of left ventricular remodeling following myocardial infarction in adult mice in vivo (2006)
  • Author(s): Miyamoto, S., Kawamura, T., Morimoto, T., Ono, K., Wada, H., Kawase, Y., Matsumori, A., Nishio, R., Kita, T., Hasegawa, K
  • Journal Title: Circulation 113 Pages: 679-90
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Three catheter-based strategies for cardiac delivery of therapeutic gelatin microspheres. (2006)
  • Author(s): Hoshino K, Kimura T, De Grand AM, Yoneyama R, Kawase Y, Houser S, Ly HQ, Kushibiki T, Furukawa Y, Ono K, Tabata Y, Frangioni JV, Kita T, Hajjar RJ, Hayase M.
  • Journal Title: Gene Ther. 13 Pages: 1320-1327
• [Journal Article] Histone acetyltransferase activity of p300 is required for the promotion of left ventricular remodeling (2006)
  • Author(s): Miyamoto S, Kawamura T, Morimoto T, Ono K, Wada H, Kawase Y, Matsumori A, Nishio R, Kita T, Hasegawa K.
  • Journal Title: Circulation. 113 Pages: 679-690
• [Journal Article] TG-interacting factor is required for the differentiation of preadipocytes.
  • Author(s): Horie-T, et. al.
  • Journal Title: J Lipid Res. (In press)
  • Peer Reviewed
• [Journal Article] Oxidative stress induces GLUT4 translocation by activation of PI3-K/Akt and dual AMPK kinase in cardiac myocytes.
  • Author(s): Horie-T, et. al.
  • Journal Title: J Cell Physiol. (In press)
  • Peer Reviewed
• [Presentation] Chronic administration of endogenous inotropic peptide, apelin, prevents left ventricular remodeling and improves survival in hypertensive ratswith heart failure. (2007)
  • Author(s): Tamaki-Y, et. al.
  • Organizer: American Heart Association Annual Scientific Sessions 2007
  • Place of Presentation: Orlando, Florida
  • Year and Date: 2007-11-07
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] C-C chomokine receptor-2 inhibitor ameliorates insulin resistance and hepatic steatosis in db/db mice. (2007)
  • Author(s): Tamura-Y, et. al.
  • Organizer: American Heart Association Annual Scientific Sessions 2007
  • Place of Presentation: Orlando, Florida
  • Year and Date: 2007-11-07
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Chronic administration of endogenous inotropic peptide, apelin, prevents Ieft ventricular remodeling and improves survival in hypertensive rats with heart failure. (2007)
  • Author(s): Tamaki-Y, et. al.
  • Organizer: American Heart Association Annual Scientific Sessions 2007
  • Place of Presentation: Orlando, Florida
  • Year and Date: 2007-11-07
• [Presentation] C-C chomokine receptor-2 inhibitor ameliorates insulin resistance and hepatic stetosis in db/db mice. (2007)
  • Author(s): Tamura-Y, et. al.
  • Organizer: American Heart Association Annual Scientific Sessions 2007
  • Place of Presentation: Orlando, Florida
  • Year and Date: 2007-11-07
• [Presentation] Oxidative stress induces GLUT4 translocation by dual AMPK kinase activation in cardiac myocytes (2007)
  • Author(s): Horie-T, et. al.
  • Organizer: American Heart Association Annual Scientific Sessions 2007
  • Place of Presentation: Orlando, Florida
  • Year and Date: 2007-11-06
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] MicroRNA-206 controls cardiac myosin heavy chain gene expression by targeting RXR-α. (2007)
  • Author(s): Nishi-H, et. al.
  • Organizer: American Heart Association Annual Scientific Sessions 2007
  • Place of Presentation: Orlando, Florida
  • Year and Date: 2007-11-06
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Roles of muscle-specific microRNAs during differentiation of mouse embryonic stem cells into cardiac myocytes. (2007)
  • Author(s): Takaya-T, et. al.
  • Organizer: American Heart Association Annual Scientific Sessions 2007
  • Place of Presentation: Orlando, Florida
  • Year and Date: 2007-11-06
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Mulberry leaf ameliorates the production of adipocytokines by inhibiting oxidative stress in white adipose tissue in db/db mice. (2007)
  • Author(s): Sugimoto-M, et. al.
  • Organizer: American Heart Association Annual Scientific Sessions 2007
  • Place of Presentation: Orlando, Florida
  • Year and Date: 2007-11-06
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Cyclin-dependent kinase-9, as a novel component of p300/GATA4 complex, is involved in the differentiation of mouse ES cells into cardiac myocytes. (2007)
  • Author(s): Kaichi-S, et. al.
  • Organizer: American Heart Association Annual Scientific Sessions 2007
  • Place of Presentation: Orlando, Florida
  • Year and Date: 2007-11-06
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Oxidative stress induces GLUT4 transIocation by dual AMPK kinase activation in cardiac myocytes (2007)
  • Author(s): Horie-T, et. al.
  • Organizer: American Heart Association Annual Scientific Sessions 2007
  • Place of Presentation: Orlando, Florida
  • Year and Date: 2007-11-06
• [Presentation] MicroRNA-206 controls cardiac myosin heavy chain gene expression by targeting RXR-α. (2007)
  • Author(s): Nishi-H, et. al.
  • Organizer: America Heart Association Annual Scientific Sessions 2007
  • Place of Presentation: Orlando, Florida
  • Year and Date: 2007-11-06
• [Presentation] Serotonin in adipose tissue acts as an autocrine factor required for adipocyte differentiation and lipolysis. (2007)
  • Author(s): Ono-K, et. al.
  • Organizer: American Heart Association Annual Scientific Sessions 2007
  • Place of Presentation: Orlando, Florida
  • Year and Date: 2007-11-05
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Activation of smad transcriptional coreppressor TGIF by insulin is required for adipocyte differentiation. (2007)
  • Author(s): Horie-T, et. al.
  • Organizer: American Heart Association Annual Scientific Sessions 2007
  • Place of Presentation: Orlando, Florida
  • Year and Date: 2007-11-05
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] MicroRNA-133 regulates the expression of CPT-1b and GLUT4 by targeting SRF and KLF15 and is involved in metabolic control in cardiac myocytes. (2007)
  • Author(s): Horie-T, et. al.
  • Organizer: American Heart Association Annual Scientific Sessions 2007
  • Place of Presentation: Orlando, Florida
  • Year and Date: 2007-11-05
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Serotonin in adipose tissue acts as an autocrine factor required for adipocyte differentiation and lipolysis. (2007)
  • Author(s): Ono-K, et. al.
  • Organizer: American Heart Association Annnal Scientific Sessions 2007
  • Place of Presentation: Orlando, Florida
  • Year and Date: 2007-11-05
• [Presentation] Activation of smad transcriptional coreppressor TGIF byinsulin is required for adipocyte differentiation. (2007)
  • Author(s): Horie-T, et. al.
  • Organizer: American Heart Association Annual Scientific Sessions 2007
  • Place of Presentation: Orlando, Florida
  • Year and Date: 2007-11-05
• [Presentation] Serotonin in adipose tissue acts as an autocrine factor required for adipocyte differentiation and Iipolysis (2007)
  • Author(s): Ono, K., Horie, T., Abe, Y., Kawamura, T., Kinoshita, M., Nishi, H., Nagao, K., Satoh, N., Shimatsu, A., Kita, T., Hasegawa, K
  • Organizer: American Heart Association Annual Scientific Sessions
  • Place of Presentation: Orlando, Florida
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Activation of smad transcriptional coreppressor TGIF by insulin is required for adipocyte differentiation (2007)
  • Author(s): Horie, T., Ono, K., Abe, Y., Kawamura, T., Shimatsu, A., Kita, T., Hasegawa, K
  • Organizer: American Heart Association Annual Scientific Sessions
  • Place of Presentation: Orlando, Florida
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] MicroRNA-133 regulates the expression of CPT-lb and GLUT4 by targeting SRF and KLF15 and is involved in metabolic control in cardiac myocytes (2007)
  • Author(s): Horie, T., Ono, K., Iwanaga, Y., Nishi, H., Nagao, K., Kinoshita, M., Mori, R., Hasegawa, K., Kita, T
  • Organizer: American Heart Association Annual Scientific Sessions
  • Place of Presentation: Orlando, Florida
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Oxidative stress induces GLUT4 translocation by dual AMPK kinase activation in cardiac myocytes (2007)
  • Author(s): Hone, T., Ono, K., Abe, Y., Kawamura, T., Wada, H., Kinoshita, M., Nagao, K., Nishi, H., Shimatsu, A., Kita, T., Hasegawa, K
  • Organizer: American Heart Association Annual Scientific Sessions
  • Place of Presentation: Orlando, Florida
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] MicroRNA-206 controls cardiac myosin heavy chain gene expression by targeting RXR-α (2007)
  • Author(s): Nishi, H., Ono, K., Iwanaga, Y., Hone, T., Kinoshita, M., Nagao, K., Mon, R., Hasegawa, K., Kita, T
  • Organizer: American Heart Association Annual Scientific Sessions
  • Place of Presentation: Orlando, Florida
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Roles of muscle-specific microRNAs during differentiation of mouse embryonic stem cells into cardiac myocytes (2007)
  • Author(s): Takaya-T., Kawamura, T., Ono, K., Mori, R., Kaichi, S., Kita, T., Wada, H., Morimoto, T., Shimatsu, A., Hasegawa, K
  • Organizer: American Heart Association Annual Scientific Sessions
  • Place of Presentation: Orlando, Florida
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Mulberry leaf ameliorates the production of adipocytokines by inhibiting oxidative stress in white adipose tissue in db/db mice (2007)
  • Author(s): Sugimoto, M., Arai, H., Tamura, Y., Murayama, T., Ono, K., Ariyasu, H., Akamizu, T., Kita, T., Kamei, K., Yokode, M
  • Organizer: American Heart Association Annual Scientific Sessions
  • Place of Presentation: Orlando, Florida
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Cyclin-dependent kinase-9, as a novel component of p300/GATA4 complex, is involved in the differentiation of mouse ES cells into cardiac myocytes (2007)
  • Author(s): Kaichi, S., Takaya, T., Morimoto, T., Sunagawa, Y., Kawamura, T., Ono, K., Rita, T., Hidaka, K., Morisaki, T., Baba, S., Heike, T., Nakahata, T., Hasegawa, K
  • Organizer: American Heart Association Annual Scientific Sessions
  • Place of Presentation: Orlando, Florida
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Chronic administration of endogenous inotropic peptide, apelin, prevents left ventricular remodeling and improves survival in hypertensive rats with heart failure (2007)
  • Author(s): Tamaki, Y., Iwanaga, Y., Niizuma, S., Kawashita, T., Kato, T., Inuzuka, Y., Ono, K., Shioi, T., Kita, T
  • Organizer: American Heart Association Annual Scientific Sessions
  • Place of Presentation: Orlando, Florida
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] C-C chomokine receptor-2 inhibitor ameliorates insulin resistance and hepatic steatosis in db/db mice (2007)
  • Author(s): Tamura, Y., Arai, H., Sugimoto, M., Murayama, T., Ueda, Y., Kanamori, H., Ono, K., Ariyasu, H., Akamizu, T., Kita, T., Yokode, M
  • Organizer: American Heart Association Annual Scientific Sessions
  • Place of Presentation: Orlando, Florida
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Identification of signaling pathways that mediate the oxidative stress-induced translocation of myocardial GLUT4 using a novel lenti viral-mediated GLUT4 reporter gene (2006)
  • Author(s): Takahiro, Horie, Koh, Ono, Yukiko, Abe, Teruhisa, Kawamura, Akira, Shimatsu, Koji, Hasegawa, Toru, Kita
  • Organizer: American Heart Association Annual Scientific Sessions
  • Place of Presentation: Chicago, Illinois Department of Cardiovascular Medicine, Kyoto University Division of Translational Research, Kyoto Medical Center, National Hospital Organization
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Soluble Flk-1 and Soluble Tie-2, Endogenous Inhibitors of Endothelial Cell-specific Angiogenic Singals, are Increased in Serum of Patients with Metabolic Syndrome (2006)
  • Author(s): Hiromichi, Wada., Koh, Ono, Noriko, Satoh, Sachika, Kameda, Daisuke, Shinjo, Yukiko, Abe, Teruhisa, Kawamura, Tatsuya, Morimoto, Tameo Nakano, Akira, Shimatsu, Masatoshi, Fujita, Koji, Hasegawa
  • Organizer: American Heart Association Annual Scientific Sessions
  • Place of Presentation: Chicago, Illinois
  • Description: 「研究成果報告書概要(欧文)」より
• [Remarks] 「研究成果報告書概要(和文)」より
  • URL: http://www.kuhp.kyoto-u.ac.jp/~cardiovasc_med/kenkyushitsu/kenkyushitsu_metabolic.html
• [Remarks]
  • URL: http://www.kuhp.kyoto-u.ac.jp/~cardiovasc_med/kenkyushitsu/kenkyushitsu_metabolic.html
• [Patent(Industrial Property Rights)] ユース特許(肥満の予防及び/又は治療薬) (2007)
  • Inventor(s): 尾野 亘
  • Industrial Property Rights Holder: 尾野 亘
  • Industrial Property Number: 2007-024111
  • Filing Date: 2007-02-21
  • Description: 「研究成果報告書概要(和文)」より