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Screening and validation of target molecules involved in mechanism of multi-modality therapy

Research Project

Project/Area Number 18590838
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionChiba University

Principal Investigator

TAKIGUCHI Yuichi  Chiba University, Chiba University Hospital, Dept Respirol, Associate Professor (30272321)

Co-Investigator(Kenkyū-buntansha) KUROSU Kstsushi  Chiba University, Graduate School, Dept Respirol, Assistant Prof (20291106)
KASAHARA Yasunori  Chiba University, Graduate School, Dept Respirol, Assistant Prof (60343092)
SEKI Naohiko  Chiba University, Graduate School, Dept Genome Sci, Assoc Prof (50345013)
HIROSHIMA Kenzo  Chiba University, Graduate School, Dept Pathol, Assoc Prof (80218833)
KURIYAMA Takayuki  Chiba University, Graduate School, Dept Respirol, Professor (20009723)
巽 浩一郎  千葉大学, 大学院医学研究院, 助教授 (10207061)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsCisplatin / Gene expression / cDNA microarray / siRNA / Radiotherapy / Multi-modality therapy / Cell cycle / マイクロアレイ / 抗がん剤 / 放射線 / がん
Research Abstract

Background: Substantial evidence has disclosed that some cytotoxic agents have complex activities in influencing signal transduction pathways in cells. Materials and Methods: cDNA microarray analysis was performed after exposing a human squamous cell carcinoma cell line, RERF-LC-AI, to low-dose cisplatin for 5 days. Up-regulated gene expressions were suppressed by small interfering RNA to investigate phenotypic alteration of the cells. Results: Among 30,000 genes screened, 42 genes showed increases or decreases in expression of more than 2-fold in cisplatin treatment. They included genes with functions involved in apoptosis, cell cycle regulation and DNA metabolism/repair. Suppression of the most significantly altered 5 genes by small interfering RNA resulted in partly reduced apoptosis without altering cytotoxicity of cisplatin. Conclusion: Besides direct cytotoxic effects on cells, cisplatin may have indirect effects involving drug resistance, and synergistic effects with other agents.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (15 results)

All 2007 2006

All Journal Article (13 results) (of which Peer Reviewed: 4 results) Presentation (2 results)

  • [Journal Article] Altered Gene Expression by Cisplatin in a Human Squamous Cell Lung Carcinoma Cell Line.2007

    • Author(s)
      Yatomi, M, Takiguchi, Y et. al.
    • Journal Title

      Anticancer Research 27

      Pages: 3235-3244

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Interaction of cisplatin and radiation on a human lung cancer cell line according to treatment schedule.2007

    • Author(s)
      Asaka-Amano, Y, Takiguchi, Y et. al.
    • Journal Title

      Radiation Research 167

      Pages: 637-644

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Phase II study of weekly irinotecan and cisplatin for refractory or recurrent non-small cell lung cancer.2007

    • Author(s)
      Takiguchi, Y. et. al.
    • Journal Title

      Lung Cancer 58

      Pages: 253-259

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Altered Gene Expression by Cisplatin in a Human Squamous Cell Lung Carcinoma Cell Line2007

    • Author(s)
      Yatomi, M, Takiguchi, Y, et. al.
    • Journal Title

      Anticancer Research 27

      Pages: 3235-3244

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Journal Article] Interaction of cisplatin and radiation on a human lung cancer cell line according to treatment schedule2007

    • Author(s)
      Asaka-Amano, Y, Takiguchi, Y, et. al.
    • Journal Title

      Radiation Research 167

      Pages: 637-644

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Journal Article] Phase II study of weekly irinotecan and cisplatin for refractory or recurrent non-small cell lung cancer2007

    • Author(s)
      Takiguchi, Y., et. al.
    • Journal Title

      Lung Cancer 58

      Pages: 253-259

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Phase II study of weekly irinotecan and cisplatin for refractory or recurtent non-small cell lung cancer2007

    • Author(s)
      Takiguchi. Y, et. al.
    • Journal Title

      Lung Cancer 58

      Pages: 253-259

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interaction of cisplatin and radiation on a human lung cancer cell line according to treatment schedule.2007

    • Author(s)
      Asaka-Amano, Y., et al.
    • Journal Title

      Radiation Research (In press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Phase II trial of amrubicin for treatment of refractory or relapsed small-cell lung cancer2006

    • Author(s)
      Onoda, S., et al.
    • Journal Title

      Journal of Clinical Oncology 24

      Pages: 5448-53

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Docetaxel in combination with either cisplatin or gemcitabine in unresectable non-small cell lung carcinoma.2006

    • Author(s)
      Katakami, N., et al.
    • Journal Title

      Journal of Thoracic Oncology 1

      Pages: 447-53

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Basaloid squamous-cell carcinoma of the lung in a young woman.2006

    • Author(s)
      Nakagawa, H., et al.
    • Journal Title

      International Journal of Clinical Oncology 11

      Pages: 66-8

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Sequential gene expression changes in cancer cell lines after treatment with the demethylation agent 5-Aza-2'-deoxycytidine.2006

    • Author(s)
      Arai, M., et al.
    • Journal Title

      Cancer 106

      Pages: 2514-25

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Prospective stuty of adjuvant chemotherapy for pulmonary large cell neuroendocrine carcinoma.2006

    • Author(s)
      Iyoda, A., et al.
    • Journal Title

      Annals of Thoracic surgery 82

      Pages: 1802-7

    • Related Report
      2006 Annual Research Report
  • [Presentation] ヒト肺癌細胞株を用いたシスプラチンによる遺伝子発現変化とその細胞毒性効果の検討2007

    • Author(s)
      弥富真理、滝口裕一、他
    • Organizer
      第5回日本臨床腫瘍学会学術集会
    • Place of Presentation
      札幌
    • Year and Date
      2007-03-23
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Altered gene expression and cytotoxicity in a cisplatin treated human squamous cell lung cancer cell line2007

    • Author(s)
      Yatomi, M, Takiguchi, Y, et. al.
    • Organizer
      The 5th Japanese Society for Medical Oncology
    • Place of Presentation
      Sapporo, Japan.
    • Year and Date
      2007-03-23
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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