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Cellular differentiation from human lung epithelial progenitor cells to pulmonary nuroendocrine cells

Research Project

Project/Area Number 18590868
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionTokyo University of Pharmacy and Life Science

Principal Investigator

TAKAHASHI Yuji  Tokyo University of Pharmacy and Life Science, School of Life Sciences, Professor (20154875)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Shigeru  Tokyo University of Pharmacy and Life Sciences, School of Life Science, Associate Professor (10266900)
HIROSE Hidenori  Tokyo University of Pharmacy and Life Sciences, School of Life Science, Research Associate (80398817)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,860,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsNeuroendocrine cells / pulmonary epithelial cells / hypoxia / cellular differentiation / コラーゲン / 神経内分泌
Research Abstract

Lung epithelial neuroendocrine cells are differenciated from multipotent stem cell in lung at the mid term of the pregnancy in human. Pulmonary neuroendocrine cells secret catecholamine and neural peptides, resulting in the regulation of the proliferation and differentiation of lung epithelial cells. It could be possible that neuroendocrine cell differentiation may be associate to pathogenesis of lung diseases. We hypothesized that Ash1 might modulate the differentiation of neuroendocrine cell. To understand the mechanism of pulmonary neuroendocrine cell, we established the in virto cell culture system in which multi-potent stem cell of lung epithelium can differentiate to neuroendocrine-like cells. Interaction collagen matrix and hypoxic condition are important conditions that differentiate the premature pulmonary epithelial cells to neuroendocrine cells. Gene expression profiles under cell differentiation indicated that biphasic up-regulation of Ash1 might be critical for the determination of the epithelial cells for the neuroendocrine differentiation. In these processes, Ash1 regulated the target genes. To assess the role of Ash1 in this process, we tried to produce SiRNA to knockdown Ash1 mRNA. Knockdown of Ash1 expression repressed the cellular differentiation of progenitor cells to neuroendocrine cells and repress typrosine hydroxylase transcription. Our research, now, is ongoing to identify the mechanism of Ash1-regulated gene expression with association to the pulmonary neuroendocrine cell differentiation.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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