| Project/Area Number |
18590869
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Nippon Medical School |
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Principal Investigator |
KUDOH Shoji Nippon Medical School, Department of Internal Medicine, Professor (40256912)
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| Co-Investigator(Kenkyū-buntansha) |
GEMMA Akihiko Nippon Medirsil School, Department of Internal Medicine, Associate Prof (20234651)
OKANO Tetsuya Nippon Medical School, Department of Internal Medicine, Assistant Prof (00339376)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,950,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Chronic epithelium damage / Lung cancer / IPF / TGF-β / EMT / 発癌分子機構 / cDNA / 抗体アレイ / 2D-DIGE |
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| Research Abstract |
Patients with chronic epithelium damage have an increased risk of developing lung cancer. To identify key molecules involved in malignant transformation in these patients, we analyzed the expression profiles of lung tumor and lung tissue from these patients using cDNA arrays and real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Altered expressions of the genes downstream of transforming growth factor-β (TGF-β) were identified in tumor samples from lung cancer patients with IPF, in which TGF-β is overexpressed, using real-time RT-PCR. Our findings indicate that the inactivation of TGF-β signaling pathway may play an important role in pulmonary carcinogensis or progression of lung cancer in the patients with chronic epithelium damage. In addition, we analyzed the change of the expression profiles after TGF-β exposure in TGF-β-sensitive epithelial cells using cDNA array, 2D-DIGE, and Ab-array. Many molecules were identified, expression levels of which were increased or decreased after TGF-β exposure. One of those is concerned with EMT. Further study of the relationship of this molecule and Smads is required. These findings could be used to develop preventive measures or treatment of lung cancer patients with chronic epithelium damage.
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