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EFFCTS OF ANOVEL NTERACTING MOLECULE OFAT1 RECEPTOR ON RENAL FUNCTION

Research Project

Project/Area Number 18590897
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionYokohama City University

Principal Investigator

TAMURA Kouichi  Yokohama City University, GARDUATE SCHOOL OF MEDICINE, DEPARTMENT OF CARDIORENAL MEDICINE, ASSOCIATE PROFESSOR (40285143)

Co-Investigator(Kenkyū-buntansha) HORIUCHI Masatsugu  EHIME UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF MEDICAL BIOCHEMISTRY, CHAIRMAN & PROFESSOR (40150338)
UMEMURA Satoshi  YOKOHAMA CITY UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, DEPARTMENT OF MEDICAL SCIENCE AND CARDIORENAL MEDICINE, CHAIRMAN & PROFESSOR (00128589)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,880,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥480,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsSignaling / Blood pressure regulation / Interacting molecule / Bioactivity / Translational research
Research Abstract

Chronic elevations of circulating angiotensin II (Ang II) cause sustained hypertension and enhanced accumulation of intrarenal Ang II by an Ang II type 1 receptor (AT1 receptor) -dependent process. Previous studies showed that the C-terminal cytoplasmic domain of AT1 receptor is involved in the control of receptor internalization and in linking receptor-mediated signal transduction to the specific biological response. We previously cloned a novel molecule ATRAP (AT1 receptor-associated protein) that specifically interacts with C-terminal of AT1 receptor. The results of previous in vitro studies showed that ATRAP specifically inhibits AT1 receptor signaling by constitutive activation of AT1 receptor internalization to decrease cell surface AT1 receptor number. The present study tested the hypothesis that chronic elevations in circulating Ang II regulate ATRAP protein expression in a tissue-specific manner. C57BL6 mice were infused with Ang II (1,000 ng/kg/min)or vehicle subcutaneously f … More or 14 days via osmotic minipump. On day 12, systolic blood pressure averaged 176+/-1.0 mm Hg in Ang II-infused mice compared with mice given vehicle (122+/-4 mm Hg) .Western blot analysis using the anti-AT1 receptor and anti-ATRAP antibodies was performed. The results showed that AT1 receptor protein levels in the kidney and liver were comparable in Ang II- and vehicle-infused mice. In contrast, ATRAP protein levels were significantly decreased in the kidney of Ang II-infused mice (51% decrease; P<0.05). ATRAP protein levels in the liver were also similar in the two groups. Therefore, these "results indicate that renal and liver AT1 receptor gene expression is maintained but renal ATRAP gene expression is specifically suppressed in Ang II-induced hypertension. The renal-specific down-regulation of the ratios of ATRAP/AT1 receptor expression by Ang II infusion causes the relative predominance of AT1 receptor signaling over inhibition by ATRAP in the kidney and thus allows the sustained effects of chronic elevations in Ang II to elicit progressive increases in blood pressure. Less

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (20 results)

All 2008 2007 2006 2005

All Journal Article (15 results) (of which Peer Reviewed: 6 results) Presentation (2 results) Book (3 results)

  • [Journal Article] Novel regulatory effect of angiotensin II type 1 receptor-interacting molecule on vascular smooth muscle cells2007

    • Author(s)
      Azuma K, Tamura K, et. al.
    • Journal Title

      Hypertension 50

      Pages: 926-932

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Expression of MAK-V/Hunk in renal distal tubules and its possible involvement in proliferative suppression2007

    • Author(s)
      Sakai M, Tamura K, lkeya Y, et. al.
    • Journal Title

      Am J Physiol Renal Physiol 292

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The role of angiotensin AT1 receptor-associated protein in renin-angiotensin system regulation and function2007

    • Author(s)
      Tamura K, lkeya Y, et. al.
    • Journal Title

      Curr Hypertens Rep 9

      Pages: 121-127

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] regulatory effect of angiotensin II type 1 receptor-interacting molecule on vascular smooth muscle cells2007

    • Author(s)
      Azuma, K., Tamura, K., Shigenaga, A., Wakui, H., Masuda, S.,Tsurumi-lkeya, Y., Tanaka, Y., Sakai, M., Matsuda, M., Hashimoto, T., Ishigami, T., Lopez-llasaca, M., Umemura, S
    • Journal Title

      Hypertension 50

      Pages: 926-932

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Expression of MAK-V/Hunk in renal distal tubules and its possible involvement in proliferative suppression2007

    • Author(s)
      Sakai, M., Tamura, K., Tsurumi, Y., Tanaka, Y., Koide, Y., Matsuda, M., Ishigami, T., Yabana, M., Tokita, Y., Hiroi, Y., Komuro, I., Umemura, S
    • Journal Title

      Am J Physiol Renal Physiol 292

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The role of angiotensin AT1 receptor-associated protein in renirr-angiotensin system regulation and function2007

    • Author(s)
      Tamura, K., Tanaka, Y., Tsurumi, Y., Azuma, K., Shigenaga, A., Wakui, H., Masuda, S., Matsuda, M
    • Journal Title

      Curr Hypertens Rep 9

      Pages: 121-127

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Novel regulatory effect of angiotensin II type 1 receptor-interacting molecule on vascular smooth muscle cells.2007

    • Author(s)
      Azuma K, Tamura K, et. al.
    • Journal Title

      Hypertension 50

      Pages: 926-932

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of MAK-V/Hunk in renal distal tubules and its possible involvement in proliferative suppression.2007

    • Author(s)
      Sakai M, Tamura K, Ikeya Y, et. al.
    • Journal Title

      Am J Physiol Renal Physiol. 292

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The role of angiotensin AT1 receptor-associated protein in renin-angiotensin system regulation and function.2007

    • Author(s)
      Tamura K, Ikeya Y, et. al.
    • Journal Title

      Curr Hypertens Rep 9

      Pages: 121-127

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interacting Molecule of Angiotensin II Type 1 Receptor, ATRAP, is Co-localized with AT1 Receptor in the Mouse Renal Tubules.2006

    • Author(s)
      Tsurumi Y, Tamura K, et al.
    • Journal Title

      Kidney International 69

      Pages: 488-494

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Transcriptional diversity and expression of NEDD4L gene in distal nephron.2006

    • Author(s)
      Umemura M, Tamura K, et al.
    • Journal Title

      Biochem Biophys Res Commun 339

      Pages: 1129-1137

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Chronic activation of the prostaglandin receptor EP4 promotes hyaluronan-mediated neointimal formation in the ductus arteriosus.2006

    • Author(s)
      Yokoyama U, Tamura K, et al.
    • Journal Title

      J Clin Invest. 116

      Pages: 3026-3034

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Neuroleptic malignant syndrome induced by perospirone.2006

    • Author(s)
      Nakagawa M, Tamura K, et al.
    • Journal Title

      Int J Neuropsychopharmacol. 9

      Pages: 635-636

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Analysis of Factors that Affect Short-Term Blood Pressure Variability in Patients with Chronic Renal Failure.2005

    • Author(s)
      Sakai M, Tamura K, et al.
    • Journal Title

      Clinical and Experimental Hypertension 27

      Pages: 139-147

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The novel angiotensin II type 1 receptor (AT1R)-associated protein ATRAP downregulates AT1R and ameliorates cardiomyocyte hypertrophy.2005

    • Author(s)
      Tanaka Y, Tamura K, et al.
    • Journal Title

      FEBS Letters 579

      Pages: 1579-1586

    • Related Report
      2006 Annual Research Report
  • [Presentation] Tissue-Specific Regulation of Angiotensin II Type 1 Receptor-Interacting Molecule A丁RAP Expression in Angiotensin II-Induced Hypertension2007

    • Author(s)
      Wakui H, Tamura K, lkeya Y, et. al.
    • Organizer
      第61回米国心臓病学会高血圧評議会総会
    • Place of Presentation
      米国アリゾナ州ツーソン
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Tissue-Specific Regulation of Angiotensin II Type 1 Receptor-Interacting Molecule ATRAP Expression in Angiotensin II-Induced Hypertension.2007

    • Author(s)
      Wakui H, Tamura K, Ikeya Y, et. al.
    • Organizer
      第61回米国心臓病学会高血圧評議会総会
    • Place of Presentation
      米国アリゾナ州ツーソン
    • Related Report
      2007 Annual Research Report
  • [Book] 二次性高血圧.第12章 循環器疾患 最新の治療2008-20092008

    • Author(s)
      田村 功一, 他
    • Publisher
      南江堂(東京)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 二次性高血圧. 第12章 循環器疾患 最新の治療2008-2009.2008

    • Author(s)
      田村功一, 他。
    • Publisher
      南江堂(東京)
    • Related Report
      2007 Annual Research Report
  • [Book] THE ARB. (堀内正嗣編集)2006

    • Author(s)
      田村 功一
    • Publisher
      メディカルレビュー社(東京)
    • Related Report
      2006 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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