Establishment of Cloned kidney derived from autologous mesenchymal stem cells using xeno-embryos

• Project/Area Number: 18590911
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Jikei University School of Medicine
• Principal Investigator: YOKOO Takashi Jikei University School of Medicine, 医学部, 助教 (70301538)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,060,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥660,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: 間葉系幹細胞 / 腎臓再生 / 異種胎児 / 移植 / 異種移植 / 胎仔 / 胎児 / クローン

Research Abstract

腎臓のもつ解剖学的複雑性のため、慢性腎不全に対する再生医療はこれまでの他臓器で行われてきた再生医療研究とは異にした全く新たなアプローチが必要になると考えられる。我々はこれまで異種胎仔の発生プログラムを用いた全く新しいアプローチによってヒト骨髄由来間葉系幹細胞からネフロンへの分化誘導に成功し、さらにこのヒト由来腎臓原器をホスト動物の大網内で腎臓への分化をさらに誘導し、尿生成能も持たせることに成功した。つまりヒト由来腎臓をラット体内で機能させることが可能となった。

Research Products

• [Journal Article] Generation of transplantable erythropoietin-producer derived from human mesenchymal stem cells (2008)
  • Author(s): Yokoo T, Fukui A, Matsumoto K, Ohashi T, Sado Y, Kawamura T, Okabe M, Hosoya T, Kobayashi E.
  • Journal Title: Transplantation 85 Pages: 1654-1658
  • Peer Reviewed
• [Journal Article] Stem cells for kidney repair : Useful tool for acute renal failure? (2008)
  • Author(s): Yokoo T, et al
  • Journal Title: Kidney International 74 Pages: 847-849
  • Peer Reviewed
• [Journal Article] Generation of transplantable erythropoietin-producer derived from human mesenchymal stem cells. (2008)
  • Author(s): Yokoo T, et al
  • Journal Title: Transplantation 85 Pages: 1654-1658
  • Peer Reviewed
• [Journal Article] Stem cells and kidney organoaenesis. (2008)
  • Author(s): Yokoo T, et al.
  • Journal Title: Frontiers in Bioscience 13 Pages: 2814-2832
  • Peer Reviewed
• [Journal Article] Kidney regeneration by xeno-embryonic nephrogenesis. (2008)
  • Author(s): Yokoo T, et al.
  • Journal Title: Medical Molecular Morphology 41 Pages: 5-13
  • NAID: 10024380533
  • Peer Reviewed
• [Journal Article] Kidney organogenesis and regeneration : a new era in the treatment of chronic renal failure? (2008)
  • Author(s): Yokoo T, et al.
  • Journal Title: Clin Exp Nephrol 43 Pages: 326-331
  • Peer Reviewed
• [Journal Article] Generation of transplantable erythropoietin-producer derived from human mesenchymal stem cells (2008)
  • Author(s): Yokoo T, et. al.
  • Journal Title: Transplantation (in press)
  • Peer Reviewed
• [Journal Article] Stem cell and kidney organogenesis (2008)
  • Author(s): Yokoo T, et. al.
  • Journal Title: Frontiers in Bioscience 13 Pages: 2814-1832
  • Peer Reviewed
• [Journal Article] Kidney regeneration by xeno-embryonic nephrogenesis (2008)
  • Author(s): YokooT, et. al.
  • Journal Title: Medical Molecular Morphology (in press)
  • NAID: 10024380533
  • Peer Reviewed
• [Journal Article] Application of regenerative medicine for kidney diseases (2007)
  • Author(s): Yokoo T, Fukui A, Kobayashi E
  • Journal Title: Organogenesis 3 Pages: 34-43
  • Peer Reviewed
• [Journal Article] Emphysematous cystitis complication in a patient undergoing hemodialysis (2007)
  • Author(s): Yokoo T, Awai T, Yamazaki H, Fukuda Y, Hayashi F, Hosoya T
  • Journal Title: Clin Exp Nephrol 11 Pages: 247-250
  • NAID: 10024140974
  • Peer Reviewed
• [Journal Article] Xenobiotic kidney organogenesis from human mesenchymal stem cells using a growing rodent embryo (2006)
  • Author(s): Yokoo T, Fukui A, Ohashi T, Miyazaki Y, Utsunomiya Y, Kawamura T, Hosoya T, Okabe M, Kobayashi E
  • Journal Title: J. Am. Soc. Nephrol 17 Pages: 1026-1034
  • Peer Reviewed
• [Journal Article] Inhibition of endogenous BMP in the glomerulus leads to mesangial matrix expansion. Biochem (2006)
  • Author(s): Miyazaki Y, Ueda H, Yokoo T, Utsunomiya Y, Kawamura T, Matsusaka T, Ichikawa I, Hosoya T
  • Journal Title: Biophys. Res. Commun 340 Pages: 681-688
  • Peer Reviewed
• [Journal Article] Xenobiotic kidney organogenesis from human mesenchymal stem cells using a growing rodent embryo. (2006)
  • Author(s): Yokoo T, et al.
  • Journal Title: Journal of American Society of Nephrology 17 Pages: 1026-1034
• [Journal Article] Inhibition of endogenous BMP in the glomerulus leads to mesangial matrix expansion. (2006)
  • Author(s): Miyazaki Y et al.
  • Journal Title: Biochem. Biophys. Res. Commun. 340 Pages: 681-688
• [Presentation] Advancement in Regenerative Medicine and Stem cell Applications : Kidney Reaeneration. (2008)
  • Author(s): Yokoo T
  • Organizer: 6^<th> Asia Pacific Cord Blood Bank Consortium
  • Place of Presentation: Tokyo Japan
  • Year and Date: 2008-11-14
• [Presentation] Kidney regeneration using organogenesis of xeno-embryo (2008)
  • Author(s): Yokoo T
  • Organizer: Satellite Symposium in Toronto University
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-06-03
• [Presentation] Application of non-viral GDNF diffusion for development of chimeric rat kidney with human components (2008)
  • Author(s): Yokoo T, Fukui A, Matsumoto K, Hosoya T, Kobayashi E
  • Organizer: The American Transplant Congress
  • Place of Presentation: Toronto, Canada
• [Presentation] Application of non-viral GDNF diffusion for development of chimeric rat kidney with human components. (2008)
  • Author(s): Yokoo T, et al
  • Organizer: The American Transplant Congress
  • Place of Presentation: Toronto, Canada
• [Presentation] Renal organogenesis across the mouse to rat xenogenic barrier under the tacrolimus monotherapy. (2008)
  • Author(s): Kai K, et al
  • Organizer: 22^<nd> International Congress of the Transplantation Society
  • Place of Presentation: Sydney Australia
• [Presentation] Kidney regeneration by xeno-embryonic nephrogenesis. (2008)
  • Author(s): Yokoo T
  • Organizer: KIDSTEM International Conference
  • Place of Presentation: Liverpool UK
• [Presentation] Mesenchymal stem cells and their renal potential (2007)
  • Author(s): Yokoo T
  • Organizer: World Congress of Nephrology
  • Place of Presentation: リオデジャネイロ
  • Year and Date: 2007-04-24
• [Presentation] Kidney regeneration using an artificial thermoreversible bio-diffuser of GDNF (2007)
  • Author(s): Yokoo T, Akira F, Matsumoto K, Kawamura T, Hosoya T
  • Organizer: American Society of Nephrology 40^<th> Annual Renal Week Meeting
  • Place of Presentation: San Francisco, California, USA
• [Presentation] Mesenchymal stem cells and their renal potential (2007)
  • Author(s): Yokoo T
  • Organizer: 4^<th> World Congress of Nephrology
  • Place of Presentation: Rio de Janeiro, Brazil
• [Presentation] Generation of erythropoietin-producing self organs using human mesenchymal stem cells (2006)
  • Author(s): Yokoo T, Fukui A, Utsunomiya Y, Kawamura T, Okabe M, Hosoya T
  • Organizer: American Society of Nephrology 39^th Annual Renal Week Meeting
  • Place of Presentation: San Diego, California, USA
• [Book] Renal stem cells and kidney regeneration in Regulatory Networks in Stem cells(edited by Rajasekhar VK and Vemuri M) (2008)
  • Author(s): Yokoo T, et al
  • Total Pages: 20
  • Publisher: Humana Press INC., NJ USA
• [Book] Regulatory Networks in Stem cell (2007)
  • Author(s): Yokoo T, et. al.
  • Publisher: Humana Press INC.,NJ USA(in press)
• [Book] Renal stem cells and kidney regeneration in Regulatory Networks in Stem cells (edited by Rajasekhar VK and Vemuri M)
  • Author(s): Yokoo T, Fukui A, Matsumoto K, Kawamura T
  • Publisher: Humana Press INC., NJ USA
• [Remarks]
  • URL: http://www.yokoolab.com
• [Remarks]
  • URL: http://www.yokoolab.com