Search for inhibitory factor for polyglutamine protein ubioquitylation
Project/Area Number |
18590929
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Neurology
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Research Institution | The University of Tokyo |
Principal Investigator |
IWATA Atsushi The University of Tokyo, The University of ThkyoHospital, Assistant Professor (40401038)
|
Project Period (FY) |
2006 – 2007
|
Project Status |
Completed (Fiscal Year 2007)
|
Budget Amount *help |
¥3,650,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
|
Keywords | polyglutamine / ubiquitin / proteasome / 神経変性疾患 |
Research Abstract |
This year, we tested if any of the ubiquitin like proteins could modify polyglutamine proteins. We cloned FAT10, ubl5, FubI, Isg15 and urm1 from cDNA library and made overexpression constructs for mammalian expression. We co-expressed these constructs with polyglutamine proteins and pulled down pQ with immunoprecipitation method. Immunodetection of attached proteins revealed that FAT10 was covalently attached to pulled-down pQ proteins. This was further confirmed by pulling down pQ under denatured condition to detect FAT10 covalently bound to pQs. FAT10 is known to be homologous to ubiquitin abd known to covalently conjugated to lysine residues. We tested various amounts of FAT10 with ubiquitin co-expressed to find FAT10 affects ubiquitylation of PQ protein. By overexpressing FAT10, degradation of pQ proteins are accelerated. These data suggested that FAT10 might play and important role in pQ pathogenesis. We are now confirming the data and preparing for publication.
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Report
(3 results)
Research Products
(15 results)
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[Presentation] HDAC6とオートファジー2007
Author(s)
岩田淳
Organizer
愛知県身障者コロニーセミナー
Place of Presentation
春日井市、愛知県
Year and Date
2007-12-21
Description
「研究成果報告書概要(和文)」より
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[Presentation] HDAC6 and autophagy2007
Author(s)
Atsushi Iwata
Organizer
Aichi Colony seminar
Place of Presentation
Kasugai, Aichi
Year and Date
2007-12-21
Description
「研究成果報告書概要(欧文)」より
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[Presentation] HDAC6とオートファジー2007
Author(s)
岩田淳
Organizer
第30回日本分子生物学会
Place of Presentation
横浜市、神奈川県
Year and Date
2007-12-11
Description
「研究成果報告書概要(和文)」より
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[Presentation] HDAC6とオートファジー2007
Author(s)
岩田 淳
Organizer
HDAC/TDAC研究会
Place of Presentation
長崎大学、長崎
Year and Date
2007-08-02
Description
「研究成果報告書概要(和文)」より
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