• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Reguation of mitochondrial transcription by activated glucocorticoid receptor

Research Project

Project/Area Number 18590944
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurology
Research InstitutionThe University of Tokushima

Principal Investigator

MITSUI Takao  The University of Tokushima, University Medical and Dental Hospital, Assistant Professor (80294726)

Co-Investigator(Kenkyū-buntansha) KURODA Yukiko  University Medical and Dental Hospital, Insittute of Health Biosciences, Graduate School, Assistant member of schcol affairs (70398014)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,870,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2006: ¥2,700,000 (Direct Cost: ¥2,700,000)
Keywordsglucocorticoid / mitochondria / transcription / グリココルチコイド
Research Abstract

We found that production of the reaction oxygen species (ROS) was related to the mitochondrial dysfunctional in the skeletal muscle with a long term glucocorticoid treatment. Furthermore, because it was thought that the effect was direct action for mitochondria by the glucocorticoid, it was drawn up to clarify these points in this study more, and an interesting result was provided. We analyzed the effect of corticosteroid on the mitochondria membrane potential (ΔΨm), generation of ROS and influence of the apoptosis in various kinds of cultured cells. In the differentiated cells, the dexamethasone addition let ROS generation and apoptosis increase with a fall of ΔΨm. These changes were not seen under the existence of SOD. In the proliferating cells, the dexamethasone let ROS generation and apoptosis increase with increase of ΔΨm. The ROS production and the increase of the apoptosis disappeared under the existence of SOD, but the increase of ΔΨm did not change. These results suggested that activated glucocorticoid receptors were moved to the mitochondria in these cells. Furthermore, these indicated the existence of protein to guide to the mitochondria although it had been well recognized that the activated glucocorticoid acceptor shifted in a nucleus. We introduced the GFP protein of the glucocorticoid acceptor into cultured cell (COS, RD) to examine whether an activated glucocorticoid acceptor shifted to mitochondria under dexamethasone treatment (1 x 10^<6-> x 10^<-7>M). GFP signals were observed in the mitochondria in addition to nuclei. This strongly suggested the existence of the protein which carried a glucocorticoid acceptor to mitochondria.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (9 results)

All 2008 2007 2006

All Journal Article (8 results) (of which Peer Reviewed: 3 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Beneficial effect of tacrolimus on myasthenia gravis with thymoma2007

    • Author(s)
      T Mitsui, et. al.
    • Journal Title

      Neurologist 13

      Pages: 83-86

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Beneficial effect of tacrolimus on myasthenia gravis with thymoma2007

    • Author(s)
      T, Mitsui, et. al.
    • Journal Title

      Neurologist 13

      Pages: 83-86

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Pazkin affects mitochondrial function and apoptosis in neuronal and myogenic cells.2006

    • Author(s)
      Y Kuroda, et. al.
    • Journal Title

      Biochem Biophys Res Commun. 348

      Pages: 787-793

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Parkin enhances mitochondrial biogenesis in proliferating cells.2006

    • Author(s)
      Y Kuroda, et. al.
    • Journal Title

      Hum Mol Genet. 15

      Pages: 883-895

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Parkin affects mitochondrial function and apoptosis in neuronal and myogenic cells2006

    • Author(s)
      Y, Kuroda, et. al.
    • Journal Title

      Biochem Biophys Res Commun 348

      Pages: 787-793

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Parkin enhances mitochondrial biogenesis in proliferating cells2006

    • Author(s)
      Y, Kuroda, et. al.
    • Journal Title

      Hum Mol Genet 15

      Pages: 883-895

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Parkin enhances mitochondrial biogenesis in proliferating cells.2006

    • Author(s)
      Kuroda Y, et al.
    • Journal Title

      Hum Mol Genet : 15・6

      Pages: 883-395

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Parkin affects mitochondrial function and apoptosis in neuronal and myogenic cells.2006

    • Author(s)
      Kuroda Y, et al.
    • Journal Title

      Biochem Biophys Res Commun 348・3

      Pages: 787-793

    • Related Report
      2006 Annual Research Report
  • [Patent(Industrial Property Rights)] パーキン蛋白の新規糖化因子2008

    • Inventor(s)
      三ツ井 貴夫, 黒田 由紀子
    • Industrial Property Rights Holder
      徳島大学
    • Industrial Property Number
      2008-087534
    • Filing Date
      2008-03-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi