| Budget Amount *help |
¥2,740,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Research Abstract |
Immunoreactivity for several chemokines and for their related receptors has been demonstrated in resident cells of the central nervous system, and the up-regulation of some of them is associated with pathological changes found in herpes simplex encephalitis (HSE). We investigated the possibility of the involvement of IP-10, MCP-1, IL-8, RANTES, MIP-1a, sTNF-R1, GM-CSF, and 8-hydroxy-2'-deoxyguanosine (8OHdG) in HES pathogenesis. We studied 6 patients with HSE, 9 with non-herpetic limbic encephalitis (NHLE), 5 with aseptic meningitis (AM), 5 with acute disseminated encephalomyelitis (ADEM), and 13 with non-inflammatory neurological disease. The chemokine concentration were measured by ELISA in cerebrospinal fluid (CSF). Six chemokines levels except for GM-CSF and 8-OHdG were detectable in CSF samples from HSE, NHLE, AM, and ADEM. Comparison with HSE, NHLE, and AM, IL-8 concentration tended to increase in NHLE, and RANTES, MIP-1a concentration tended to increase in AM. However, statistical difference was not seen between these groups. IP-10 concentration was significantly increased in ADEM, whereas MCP-1 level was decreased in ADEM. These findings of increased IP-10 and decreased MCP-1 were thought to be features of demyelinated neurological disease. In the present study, anti-glutamate receptorantibodies (G1uR Ab) in CSF were measured. GluR Ab were positive in 5 cases (3 with NHLE, 1 with HSE, 1 with ADEM), and were negative in 4 cases (3 with NHLE, 1 with HSE). Compared with these groups, all chemokine levels tended to elevated in the GluR Ab positive group. Various and specific chemokines were present in the CSF in response to inflammatory reaction or production of auto-immune antibodies.
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