Anti-atherosclerotic factors induced by Smad signaling in vascular cells
| Project/Area Number |
18590976
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Chiba University |
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Principal Investigator |
YOKOTE Koutaro Chiba University, Graduate School of Medicine, Associate professor (20312944)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Atherosclerosis / TGF-beta / macrophage / Smad3 / MCP-1 / inflammation / Smad / 細胞内シグナル伝達 / プラーク / 骨髄移植 |
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| Research Abstract |
Aim: To investigate the molecular basis of TGF-beta signaling involved in its anti-atherosclerotic effect.
Methods and Results: Double knockout mice for ApoE and Smad3, a major intracellular signaling molecule for TGF-beta, were generated, and subjected to in vivo and in vitro analyses. The double knockout mice developed significantly larger atheromatous lesions in the aorta compared with the control apoE knockout mice. The aortic lesions of double knockout mice were rich in macrophages, scarce in collagen deposition, and highly expressed monocyte chemoattractant protein-1 (MCP-1). Inhibitory effect of TGF-beta on MCP-1 gene expression was lost in the peritoneal macrophages derived from Smad3 knockout mice. The Smad3 knockout macrophages were also resistant to growth inhibitory effect of TGF beta.
Conclusion: Our results suggest that endogenous Smad3 in macrophage has inhibitory effects on progression of atherosclerosis via modulation of inflammatory gene expression and cell growth.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Influence of C-peptide on Early Glomerular Changes in Diabetic Mice2006
Author(s)
Maezawa, Y., Yokote, K., Sonezaki, K., Fujimoto, M., Kobayashi, K., Kawamura, H., Tokuyama, T., Takemoto, M., Ueda S, Kuwaki, T., Mori, S., Wahren, J., Saito, Y.
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Journal Title
Diabetes Metab. Res. Rev 22
Pages: 313-322
Description
「研究成果報告書概要(欧文)」より
Related Report
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