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The role of Notch signaling in the regulation of hematopoietic stem cells in the niche

Research Project

Project/Area Number 18591044
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionThe University of Tokyo

Principal Investigator

KUMANO Keiki  The University of Tokyo, The University of TokyoHospital, Assistant Professor (90396721)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsNotch / Hematopoietic stem ce / niche / 血液内科 / 幹細胞
Research Abstract

1) Estimation of hematopoietic stem cells (HSCs) in the reduced Notch signaling
We evaluated the HSCs of the Notch1+/-Notch2+/- mice or Notch1-/-Notch2-/- mice induced by the Mx1-cre transgene. Using FACS analysis, there was no difference in the number of CD34(-)KSL cells in the steady state or proliferative state induced by the 5-FU administration compared to the control mice.
In addition, we could not find the change of the number of HSCs by in vivo administration of g-secretase inhibitor (GSI), which is the inhibitor of Notch signaling. Quantification of HSCs was performed by the FACS analysis and transplantation.
2) The role of Notch signaling in the in vitro expansion of HSCs.
Inhibition of Notch signaling by GSI could impair the expansion of KSL cells, which include the HSCs, in the in vitro culture with FGF.
These results indicate that the dependency of HSCs to the Notch signaling differ between in vitro and in vivo.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (17 results)

All 2008 2006

All Journal Article (10 results) (of which Peer Reviewed: 4 results) Presentation (6 results) Book (1 results)

  • [Journal Article] Both Notch1 and Notch2 contribute to the regulation of melanocyte homeostasis.2008

    • Author(s)
      Kumano K, Masuda S, Sata M, et. al.
    • Journal Title

      Pigment Cell and Melanoma Research 21

      Pages: 70-78

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Both Notch1 and Notch2 contribute to the regulation of melanocyte homeostasis2008

    • Author(s)
      Kumano K, Masuda S, Sata M, et. al.
    • Journal Title

      Pigment Cell and Melanoma Research 21

      Pages: 70-78

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Both Notch1 and Notch2 contribute to the regulation of melanocyte homeostasis.2008

    • Author(s)
      Kumano K, Masuda S, Sata M eta al.
    • Journal Title

      Pigment Cell Melanoma Research 21

      Pages: 70-78

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] AML1/Runx1 rescues Notch1-null mutation-induced deficiency of para-aortic splanchnopleural hematopoiesis.2006

    • Author(s)
      Nakagawa M, Ichikawa M, Kumano K, et. al.
    • Journal Title

      Blood. 108

      Pages: 3329-3334

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Highly efficient ex vivo expansion of human hematopoietic stem cells using Delta1-Fc chimeric protein.2006

    • Author(s)
      Suzuki T, Yokoyama Y, Kumano K, et. al.
    • Journal Title

      Stem Cells. 24

      Pages: 2456-2465

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] AML1/Runx1 rescues Notch 1-null mutation-induced deficiency of para-aortic splanchnopleural hematopoiesis2006

    • Author(s)
      Nakagawa M, Ichikawa M, Kumano K, et. al.
    • Journal Title

      Blood 108

      Pages: 3329-3334

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Highly efficient ex vivo expansion of human hematopoietic stem cells using Deltal-Fc chimeric protein2006

    • Author(s)
      Suzuki T, Yokoyama Y, Kumano K, et. al.
    • Journal Title

      Stem Cells 24

      Pages: 2456-2465

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Highly efficient ex vivo expansion of human hematopoietic stem cells using Delta1-Fc chimeric protein2006

    • Author(s)
      Suzuki, T.
    • Journal Title

      Stem Cells 24

      Pages: 2456-2456

    • Related Report
      2006 Annual Research Report
  • [Journal Article] AML1/Runx1 rescues Notch1-null mutation-induced deficiency of para-aortic splanchnopleural hematopoiesis2006

    • Author(s)
      Nakagawa, M.
    • Journal Title

      Blood 198

      Pages: 3239-3239

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Notchシグナルと造血器腫瘍2006

    • Author(s)
      熊野 恵城
    • Journal Title

      血液・腫瘍科 52

      Pages: 639-639

    • Related Report
      2006 Annual Research Report
  • [Presentation] Notch1およびNotch2による色素幹細胞の制御2006

    • Author(s)
      熊野恵城、増田茂夫, ら
    • Organizer
      第4回幹細胞シンポジウム
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Notch1およびNotch2による色素幹細胞の制御2006

    • Author(s)
      熊野恵城、増田茂夫, ら
    • Organizer
      第27回 日本炎症・再生医学会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Both Notch1 and Notch2 contribute to the regulation of melanocyte stem cells.2006

    • Author(s)
      Kumano K, Masuda S, Sata M, et. al.
    • Organizer
      4th International Society for Stem Cell Research: 2006 Annual Meeting
    • Place of Presentation
      Toronto, Canada
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Both Notch1 and Notch2 contribute to the regulation of melanocyte stem cells2006

    • Author(s)
      Kumano K, Masuda S, Sata M, et. al.
    • Organizer
      4th stem cell symposium
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Kumano K, Masuda S, Sata M, et. al., Both Notch1 and Notch2 contribute to the regulation of melanocyte stem cells2006

    • Author(s)
      Kumano K, Masuda S, Sata M, et. al.
    • Organizer
      27th Annual meeting of Japanese Society of inflammation and regenerative medicine
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Kumano K, Masuda S, Sata M, et. al., Both Notch1 and Notch2 contribute to the regulation of melanocyte stem cells2006

    • Author(s)
      Kumano K, Masuda S, Sata M, et. al.
    • Organizer
      4th International Society for Stem Cell Research : 2006 Annual Meeting
    • Place of Presentation
      Toronto, Canada
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Annual Review 血液20082008

    • Author(s)
      熊野恵城、黒川峰夫
    • Total Pages
      9
    • Publisher
      中外医学社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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