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Development of new targeted therapy ford thrombosis or cancer based on a molecudar mechanism of iniegrin activation signaling.

Research Project

Project/Area Number 18591057
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionOsaka University

Principal Investigator

TADOKORO Seiji  Osaka University, Hospital, Resident (80403062)

Co-Investigator(Kenkyū-buntansha) TOMIYAMA Yoshiaki  Osaka Univetsty, Hospital, Associate Professor (80252667)
KANAKURA Yuzuru  Osaka Univetsty, Graduate School of Medione, Professor (20177489)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsintegrin / signal transduction / platelet / cancer / targeted therapy / 活性化シグナル / 抗血小板薬 / 抗腫瘍薬
Research Abstract

【Development of new targeted therapy for thrombosis or cancer】
We have reported that talin is required for αIIβ33 activation downstream of a number of physiologically relevant signaling pathways. In this project we chose talin as a new potential target for antiplatelet therapy. Palmitoylated peptides based on the sequence of human 33 were synthesized to block talin-β3 interaction. This peptide inhibited platelet aggregation induced protease-activated receptor 1- activating peptide (PAR1-AP) or PAR4-AP. It also brought about sequence specific dose-dependent inhibition of PAC-1 binding without affecting granular secretion. These inhibitory effects were observed in both washed platelets and platelet rich plasma. These peptides might be an effective tool not only developing drug but also investigating short signaling motifs in molecular signaling pathways.
Molecular mechanism of integrin activation signaling】
Integrin activation is regulated by many different biochemical signaling pathways th … More rough the integrin cytoplasmic tail. The binding of the cytoskeletal proteins to integrin cytoplasmic tails leads to the conformational rearrangements of integrin extracellular domains that modulate their affinity. We proposed a new role for a-actinin in inside-out integrin activation evaluating by the time-dependent changes of activated aIIbβ3. α-Actinin was associated with αIIbβ3 in resting human platelets. Inside-out signaling from PAR dephosphorylated α-actinin and activated αIIbβ3 by changing α-actinin localization. When αIIbβ3 activation signal dwindled, α-actinin came back to αIIbβ3 with rephosphorylated. Overexpressed α-actinin increased its affinity with αIIbβ3 and inhibited PAR induced initial αIIbβ3 activation in human megakaryoblastic CMK cells- Furthermore even as α-actinin dissociated from integrin, the selectively blockade of talin-β3 interaction brought about the inhibition of αIIbβ3 activation. These observations suggest that α-actinin might play a role in setting integrins default low-affinity ligand-binding state in resting platelets and regulate αIIbβ3 activation. Less

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (30 results)

All 2007 2006

All Journal Article (12 results) (of which Peer Reviewed: 2 results) Presentation (18 results)

  • [Journal Article] Adiponectin acts as an endogenous antithrombotic factor.2006

    • Author(s)
      Kato H, Tadokoro S, et. al.
    • Journal Title

      Arterioscler Thromb Vasc Biol 26

      Pages: 224-230

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Critical role of ADP interaction with P2Y_<12> receptor in the maintenance of αIIbβ3 activation: association with Rap1B activation.2006

    • Author(s)
      Kamae T, Tadokoro S, et. al.
    • Journal Title

      J Thromb Haemost 4

      Pages: 1379-1387

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Adiponectin acts as an endogenous antithrombotic factor2006

    • Author(s)
      Kato, H, Tadokoro, S, et. al.
    • Journal Title

      Arterioscler Thromb Vase Biol 26

      Pages: 224-230

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Critical role of ADP interaction with P2Y_<12>, receptor in the maintenance of aIIbβ3 activation : association with Rap1B activation2006

    • Author(s)
      Kamae, T, Tadokoro, S, et. al.
    • Journal Title

      J Thromb Haemost 4

      Pages: 1379-1387

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Adiponectin acts as an endogenous antithrombotic factor.2006

    • Author(s)
      Hisashi Kato et al.
    • Journal Title

      Arteriosclerosis, Thrombosis, and Vascular Biology 26・1

      Pages: 224-230

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Critical role of ADP interaction with P2Y12 receptor in the maintenance of αIIbβ3 activation : association with Rap1B activation.2006

    • Author(s)
      Tsuyoshi Kamae et al.
    • Journal Title

      J Thromb Haemost 4・6

      Pages: 1379-1387

    • Related Report
      2006 Annual Research Report
  • [Journal Article] P2Y12-Independent Transient activation and P2Y12-Dependent Prolonged Activation of Platelet Integrin αIIbβ3.2006

    • Author(s)
      Masamichi Shiraga et al.
    • Journal Title

      Blood 108・11

    • Related Report
      2006 Annual Research Report
  • [Journal Article] インテグリンαIIbβ3活性化におけるP2Y12の重要性-巨核球系CMKを用いた解析-2006

    • Author(s)
      白鹿 正通ら
    • Journal Title

      臨床血液 47・9

      Pages: 337-337

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Semaphorin 3AはPI3 kinaseを介して血小板機能を抑制する2006

    • Author(s)
      秋山 正夫ら
    • Journal Title

      臨床血液 47・9

      Pages: 338-338

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 本邦における血小板無力症の遺伝子異常2006

    • Author(s)
      釜江 剛ら
    • Journal Title

      臨床血液 47・9

      Pages: 338-338

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 培養巨核球におけるインテグリンαIIbβ3活性化は一過性である。2006

    • Author(s)
      白鹿 正通ら
    • Journal Title

      日本血栓止血学会誌 17・5

      Pages: 580-580

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Semaphorin 3AはPI3 kinaseを介して血小板機能を抑制する。2006

    • Author(s)
      秋山 正夫ら
    • Journal Title

      日本血栓止血学会誌 17・5

      Pages: 587-587

    • Related Report
      2006 Annual Research Report
  • [Presentation] Talinとα-acthninによる血小板インテグリンαIIbβ3活性化調節機構の解析2007

    • Author(s)
      田所 誠司, ら
    • Organizer
      第69回日本血液学会総会・第49回日本臨床血液学会総会 合同開催
    • Place of Presentation
      横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] α-Actininによる血小板インテグリンαIIbβ3活性化制御機構の解析2007

    • Author(s)
      田所 誠司, ら
    • Organizer
      第30回日本血栓止血学会学術集会
    • Place of Presentation
      三重
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Semaphorin 3A antagonizes agonist-induced PI3 kinase pathway in platelets.2007

    • Author(s)
      Akiyama M, Tadokoro S, et. al.
    • Organizer
      XXI Congress of the International Society on Thrombosis and Haemostasis
    • Place of Presentation
      Geneva, Switzerland
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Semaphorin 3A inhibits platelet function via PI3 kinase-dependent pathways2007

    • Author(s)
      Akiyama M, Tadokoro S, et. al.
    • Organizer
      The 5th Aso International Meeting
    • Place of Presentation
      Aso, Japan
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] (Oral) Dynamic regulation of platelet integrin aIIbβ3 activation by talin and a-actinin2007

    • Author(s)
      Tadokoro, S, et. al.
    • Organizer
      The Japanese Society of Hematology 69th Annual meeting
    • Place of Presentation
      Yokohama, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] (Oral) A role for a-actinin in platelet integrin aIIbβ3 activation2007

    • Author(s)
      Tadokoro, S, et. al.
    • Organizer
      The Japanese Society on Thrombosis and Haemostasis, 30th Annual meeting
    • Place of Presentation
      Mie, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] (Oral) Semaphorin 3A antagonizes agonist-induced PI3 kinase pathway in platelets2007

    • Author(s)
      Akiyama, M, Tadokoro, S, et. al.
    • Organizer
      XXI Congress of the International Society on Thrombosis and Haemostasis
    • Place of Presentation
      Geneva, Switzerland
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] (Poster) Semaphorin 3A inhibits platelet function via PI3 kinase-dependent pathways2007

    • Author(s)
      Akiyama, M, Tadokoro, S, et. al.
    • Organizer
      The 5th Aso International Meeting
    • Place of Presentation
      Aso, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Talinとα-actininによる血小板インテグリンαIIbβ3活性化調節機構の解析2007

    • Author(s)
      田所 誠司, ら
    • Organizer
      第69回日本血液学会総会・第49回日本臨床血液学会総会 合同開催
    • Place of Presentation
      横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] Semaphorin 3A inhibits platelet function via PI3 kinase-dependent pathways.2007

    • Author(s)
      Akiyama M, Tadokoro S., et. al.
    • Organizer
      The 5th Aso International Meeting
    • Place of Presentation
      Aso, Japan
    • Related Report
      2007 Annual Research Report
  • [Presentation] P2Y_<12>-independent transient activation and P2Y12-dependnet prolonged activation of platelet integrin αIIbβ3.2006

    • Author(s)
      Shiraga M, Tadokoro S, et. al.
    • Organizer
      The American Society of Hematology 48th Annual meeting
    • Place of Presentation
      Orlando, USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] インテグリンαIIbβ3活性化におけるP2Y_<12>の重要性-巨核球系細胞株CMKを用いた解析-2006

    • Author(s)
      白鹿 正通, 田所 誠司, ら
    • Organizer
      第68回日木血液学会総会・第48回日本臨床血液学会総会 合同開催
    • Place of Presentation
      福岡
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Semaphorin 3AはPI3 kinase系を介して血小板機能を抑制する2006

    • Author(s)
      秋山 正夫, 田所 誠司, ら
    • Organizer
      第68回日本血液学会総会・第48回日本臨床血液学会総会 合同開催
    • Place of Presentation
      福岡
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 本邦における血小板無力症の遺伝子異常2006

    • Author(s)
      釜江 剛, 田所 誠司, ら
    • Organizer
      第68回日本血液学会総会・第48回日本臨床血液学会総会 合同開催
    • Place of Presentation
      福岡
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] (Poster) P2Y_<12>-independent transient activation and P2Y12-dependnet prolonged activation of platelet integrin aIIbβ32006

    • Author(s)
      Shiraga, M, Tadokoro, S, et. al.
    • Organizer
      The American Society of Hematology 48th Annual meeting
    • Place of Presentation
      Orlando, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] (Poster) Roles of P2Y_<12> for integrin activation in megakaryocytic cell line, CMK2006

    • Author(s)
      Shiraga, M, Tadokoro, S, et. al.
    • Organizer
      The Japanese Society of Hematology 68th Annual meeting
    • Place of Presentation
      Fukuoka, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] (Poster) Semaphorin 3A inhibits platelet function via PI3 kinase pathways2006

    • Author(s)
      Akiyama, M, Tadokoro, S, et. al.
    • Organizer
      The Japanese Society of Hematology 68th Annual meeting
    • Place of Presentation
      Fukuoka, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] (Poster) Molecular genetic abnormalities in Glanzmann Thrombasthenia in Japan2006

    • Author(s)
      Kamae, T, Tadokoro, S, et. al.
    • Organizer
      The Japanese Society of Hematology 68th Annual meeting
    • Place of Presentation
      Fukuoka, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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