Pathway analysis in bronchial asthma
| Project/Area Number |
18591097
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | University of Tsukuba |
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Principal Investigator |
NOGUCHI Emiko University of Tsukuba, Graduate School of Comprehensive Human Sciences, Assistant Professor (40344882)
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| Co-Investigator(Kenkyū-buntansha) |
UCHIDA Kazuhiko University of Tsukuba, Graduate School of Comprehensive Human Sciences, Assistant Professor (90211078)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | asthma / microarray / proteome |
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| Research Abstract |
Asthma is the most common chronic disorder in childhood, and asthma exacerbation is an important cause of childhood morbidity and hospitalization. Allergic responses are known to be biased toward T-helper type 2 in asthmatics; however, the pathogenesis of asthma is not simple, and our understanding of the disease mechanism remains incomplete. The aim of the present study was to identify gene/protein expression signatures that reflect acute exacerbation of asthma.
Blood was taken twice from pediatric asthmatic patients, once during asthma exacerbation and once during a stable period. Blood was also taken from healthy children as a control. Total RNA was extracted from peripheral blood mononuclear cells and subjected to microarray analysis with a Takara IntelliGene HS Human Expression CHIP and an Illumina Sentrix Human-6 Expression BeadChip. Paired t-test was performed to identify genes showing altered expression during asthma exacerbation. Quantitative real-time reverse transcription-pol
… More
ymerase chain reaction was performed with 48 asthmatic children to verify the microarray results. Pathway analysis was performed with IPA version 5.0 software. Expression of 26/3 genes was significantly up/down-regulated during asthma exacerbation by microarray analysis. Many of the differentially expressed genes were involved in defense responses against pathogens and interferon induction. In pathway analysis, tumor necrosis factor and interferon gamma were centered with the highest connections in the network. Tumor necrosis factor is associated with asthma exacerbation as a potent pro-inflammatory cytokine. Interferon gamma is involved in numerous immune interactions during viral infection and contributes to antiviral mechanisms. Expression levels of IFN-related genes were higher in rhinovirus viremia positive-group than in negative-group (P<0.05).
Protein expression profiles of plasma during asthma exacerbation were analyzed by 2-DE, and 49 spots were differentially expressed during asthma exacerbation. Thirty-eight of the spots were successfully identified by MALD-TOF MS. Proteins up-or down-regulated during asthma exacerbation were involved in responses to stress and pathogens, in the complement and coagulation cascades, and in acute phase responses. Among the differentially expressed proteins, up-regulation of alpha-1-antitrypsin and complement component C7 was confirmed by nephelometry and ELISA. Our present results suggest that IFN-related genes/protease inhibitors and complement components may be involved in asthma exacerbation. Less
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Report
(3 results)
Research Products
(71 results)
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[Journal Article] Eenetic polymorphism regulating ORM1-like 3 (Saccharomyces cerevisiae)expression is associated with childhood atopic asthma in a Japanese population2008
Author(s)
Hirota T, Harada M, Sakashita M, Doi S, Miyatake A, Fujita K, Enomoto T, Ebisawa M, Yoshihara S, Noguchi E, Satio H, Nakamura Y, Tamari M
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Journal Title
J Allergy Clin Immunol 121
Pages: 769-770
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] Filaggrin null mutations are associated with atopic dermatitis and elevated levels of IgE in the Japanese population, family and case-control study2008
Author(s)
Enomoto H, Hirata K, Otsuka K, Kawai T, Takahashi T, Hirota T, Suzuki Y, Tamari M, Otsuka F, Fujieda S, Arinami T, Noguchi E
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Journal Title
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Genetic polymorphism regulating ORM1-like 3(Saccharomyces cerevisiae) expression is associated with childhood atopic asthma in a Japanese population2008
Author(s)
Hirota T, Harada M, Sakashita M, Doi S, Miyatake A, Fujita K, Enomoto T, Ebisawa M, Yoshiaa S, Noguchi E, Saito H, Nakamura Y, Tamari M
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Journal Title
J Allergy Clin Immunol 121
Pages: 769-770
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Journal Article] Genetic polymorphism regulating ORM1-like 3(Saccharomyces cerevisiae)expression is associated with childhood atopic asthma in a Japanese population2008
Author(s)
Hirota T, Harada M, Sakashita M, Doi S, Miyatake A, Fujita K, Enomoto T, Ebisawa M, Yoshihara S, Noguchi E, et. al.
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Journal Title
J Allergy Clin Immunol 121
Pages: 769-770
Related Report
Peer Reviewed
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[Journal Article] Alpha-1-antitrypsin and complement component C7 are involved in asthma exacerbation.2008
Author(s)
Nishioka T, Uchida K, Meno K, Ishii T, Aoki T, Imada Y, Makino Y, Hirata K, Matsumoto Y, Arinami T, Noguchi E
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Journal Title
Proteomics Clin Appl 2
Pages: 46-54
Related Report
Peer Reviewed
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[Journal Article] Predicition of in vitro response to interferon-alpha in renal cell carcinoma celllines2007
Author(s)
Shimazui T, Ami Y, Yoshikawa K, uchida K, Kojima T, Oikawa T, Nakamura K, Honda N, Hinotsu S, Miyazaki J, Kunita N, Akaza H
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Journal Title
Cancer Sci 98
Pages: 529-534
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] Single nucleotide polymorphism-based genome-wide linkage analysis in Japanese atopic dermatitis Families.2007
Author(s)
Enomoto H, Noguchi E, Iijima S, Takahashi T, Hayakawa K, Ito M, Kano T, Aoki T, Suzuki Y, Koga M, Tamari M, Shiohara T, Otsuka F, Arinami T
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Journal Title
BMC Dermatol 7
Pages: 5-5
NAID
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] Prediction of in vitro response to interferon-alpha in renal cell carcinoma cell lines2007
Author(s)
Shimazui T, Ami Y, Yoshikawa K, Uchida K, Kojima T, Oikawa T, Nakamura K, Honda N, Hinotsu S, Miyazaki J, Kunita N, Akaza H
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Journal Title
Cancer Sci 98
Pages: 529-534
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Single nucleotide polymorphism-based genome-wide linkage analysis in Japanese atopic dermatitis families2007
Author(s)
Enomoto H, Noguchi E, Iijima S, Takahashi T, Hayakawa K, Ito M, Kano T, Aoki T, Suzuki Y, Koga M, Tamari M, Shiohara T, Otsuka F, Arinami T
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Journal Title
BMC Dermatol 7
Pages: 5-5
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Single nucleotide polymorphism-based genome-wide linkage analysis in Japanese atopic dermatitis families2007
Author(s)
Enomoto H, Noguchi E, Iijima S, Takahashi T, Hayakawa K, Ito M, Kano T, Aoki T, Suzuki Y, Koga M, Noguchi E
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Journal Title
NAID
Related Report
Peer Reviewed
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[Journal Article] Monoallelic and unequal allelic expression of the HTR2A gene in human brain and peripheral lymphocytes2006
Author(s)
Fukuda Y, Koga M, Arai M, Noguchi E, Ohtsuki T, Horiuchi Y, Ishiguro H, Niizato K, Iritani S, Itokawa M, Arinami T
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Journal Title
Biol Psychiatry 60
Pages: 1331-1335
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Monoallelic and unequal allelic expression of the HTR2A gene in human brain and peripheral lymphocytes.2006
Author(s)
Fukuda Y, Koga M, Arai M, Noguchi E, Ohtsuki T, Horiuchi Y, Ishiguro H, Niizato K, Iritani S, Itokawa M, Arinami T
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Journal Title
Biol Psychiatry 60
Pages: 1331-1335
Related Report
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[Presentation] アレルギー疾患と遺伝2006
Author(s)
野口恵美子
Organizer
第43回日本小児アレルギー学会
Place of Presentation
千葉
Year and Date
2006-11-25
Description
「研究成果報告書概要(和文)」より
Related Report
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[Presentation] 花粉症と遺伝.2006
Author(s)
野口恵美子
Organizer
臨床研セミナー
Place of Presentation
東京
Year and Date
2006-04-28
Description
「研究成果報告書概要(和文)」より
Related Report
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